TOF1_COCIM
ID TOF1_COCIM Reviewed; 1171 AA.
AC Q1DM35; A0A0D6K9N4; J3K6H1;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Topoisomerase 1-associated factor 1;
GN Name=TOF1; ORFNames=CIMG_08628;
OS Coccidioides immitis (strain RS) (Valley fever fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Onygenales; Onygenaceae; Coccidioides.
OX NCBI_TaxID=246410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RS;
RX PubMed=19717792; DOI=10.1101/gr.087551.108;
RA Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA Henn M.R., Birren B.W., Taylor J.W.;
RT "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT and their relatives.";
RL Genome Res. 19:1722-1731(2009).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=RS;
RX PubMed=20516208; DOI=10.1101/gr.103911.109;
RA Neafsey D.E., Barker B.M., Sharpton T.J., Stajich J.E., Park D.J.,
RA Whiston E., Hung C.-Y., McMahan C., White J., Sykes S., Heiman D.,
RA Young S., Zeng Q., Abouelleil A., Aftuck L., Bessette D., Brown A.,
RA FitzGerald M., Lui A., Macdonald J.P., Priest M., Orbach M.J.,
RA Galgiani J.N., Kirkland T.N., Cole G.T., Birren B.W., Henn M.R.,
RA Taylor J.W., Rounsley S.D.;
RT "Population genomic sequencing of Coccidioides fungi reveals recent
RT hybridization and transposon control.";
RL Genome Res. 20:938-946(2010).
CC -!- FUNCTION: Forms a fork protection complex (FPC) with CSM3 and which is
CC required for chromosome segregation during meiosis and DNA damage
CC repair. FPC coordinates leading and lagging strand synthesis and moves
CC with the replication fork. FPC stabilizes replication forks in a
CC configuration that is recognized by replication checkpoint sensors (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC TOF1 and CSM3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAS29882.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; GG704913; EAS29882.2; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001241465.2; XM_001241464.2.
DR AlphaFoldDB; Q1DM35; -.
DR SMR; Q1DM35; -.
DR STRING; 246410.Q1DM35; -.
DR PRIDE; Q1DM35; -.
DR EnsemblFungi; EAS29882; EAS29882; CIMG_08628.
DR GeneID; 4560065; -.
DR KEGG; cim:CIMG_08628; -.
DR InParanoid; Q1DM35; -.
DR OrthoDB; 839367at2759; -.
DR Proteomes; UP000001261; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR044998; Timeless.
DR InterPro; IPR006906; Timeless_N.
DR PANTHER; PTHR22940; PTHR22940; 1.
DR Pfam; PF04821; TIMELESS; 1.
PE 3: Inferred from homology;
KW Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..1171
FT /note="Topoisomerase 1-associated factor 1"
FT /id="PRO_0000301736"
FT REGION 568..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 880..1171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 880..908
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 949..991
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1004..1018
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1045..1074
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1086..1105
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1171 AA; 133840 MW; 3E6A7E20D7BAA9B0 CRC64;
MEIEPPQAVE VVDPEVRSYV YSLVTALGGT AGNETGRYVL GDDALGCLRD LKKWLRFYDE
KLNRMDVARC LAECKLLNGD LVPIISLYGD AEQSDKHKAR IMLACLELLV PLTWPVEVHG
QMTFNHHRHT PYLQYSQVEY KRGILGFAPS PILRALIRVG LPSMAIPRSE RSTRDEGIIR
LMLYFFRNIA VISSPPNLPI DSDDDKATRS ATINAFQQQD VFALLLTMCS NMGNDFTFQD
VIILEILFNL VKGVDVDQLF KLNERAGTIK TDDLQDILQK EDELNREHSK NAPTRHGRFG
TMIWVKRDDE KLSTVSGQDV LKGDRATFLK MDKTKKWNKP KFKREVVDPS SNNFNLKVRL
TSSATKHLRT FVEEFLDSGF NPLFTHLRKA IEREADRVTE STSRQFWYAV SWFLHAERAR
REHQKETRQR SKGTSREIEP DSFSLVASVL NQETFVGLNR FMQHSIDFKD WQDLTAGMKC
LTQILLTVQE MAISPLEEDQ EIAENIQSRI FYEETTHDRV LSILRNYKDQ GFWYLDACTE
LAHVFLRMLE QYSKQNVDMQ VRSRRRARRK QAQAAVPNQN ETEAGGEHDS DTEDIAEAHQ
TVTERSFDFK RFSVRFCTQK SVDTFVALTK YYRELDSEQL KRAHRFFYRV AFKQDMSVLL
FRVDIISLLF KMIKGPEGLD PSKPPFKDWE ELIRQIFKKL VRKLGERPEL VVELLFSKIN
ATVFYLEYGH EKQTMSESRP ATELELKPGS ASTLDEKIRI VVSALIQEDK KPLVKWLSQV
LGSAASERLS WEMEAEARAT SSPQEQSDRS KAPSIAVLPE DDGCRTAMFR NARLRLLMRL
AGLERLDEDV LGASWMIPSS VPSSNLKEYH ELIEKHCESP AEDIDGVDPR DLMRRKRTAA
DTDSSRHEFT ENVNFGSDSE GEDDGVLFPP NLPERSKALK TLKQRRRRRR RSDDAEESGP
DEAVLEARRT AREKNALERQ RKIKSDLYVH ASDDESDEEA DIEFFAKEEM RRQAQARRVA
EALETGMPEN NTTKKKSKAS AGRKRKVKPV LELDDDDSEA SPPKRRRSDE VIESDSDGEL
MVGIGSTSPR RSQTPPTSAD NIFGSEKSPS PMFPWSVGVD QMMAKLQGKD EASADNGESN
EDEGEDVLSG TGRTRRRRTM GGFVIGSDSD S
