ID   TOF1_CRYNB              Reviewed;        1074 AA.
AC   P0CR93; Q55QA1; Q5KFW4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Topoisomerase 1-associated factor 1;
GN   Name=TOF1; OrderedLocusNames=CNBF4360;
OS   Cryptococcus neoformans var. neoformans serotype D (strain B-3501A)
OS   (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=283643;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B-3501A;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Involved in chromosome segregation during meiosis and DNA
CC       damage repair. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AAEY01000032; EAL20110.1; -; Genomic_DNA.
DR   RefSeq; XP_774757.1; XM_769664.1.
DR   AlphaFoldDB; P0CR93; -.
DR   SMR; P0CR93; -.
DR   EnsemblFungi; EAL20110; EAL20110; CNBF4360.
DR   GeneID; 4936988; -.
DR   KEGG; cnb:CNBF4360; -.
DR   VEuPathDB; FungiDB:CNBF4360; -.
DR   HOGENOM; CLU_004294_1_0_1; -.
DR   Proteomes; UP000001435; Chromosome 6.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR044998; Timeless.
DR   InterPro; IPR006906; Timeless_N.
DR   PANTHER; PTHR22940; PTHR22940; 1.
DR   Pfam; PF04821; TIMELESS; 1.
PE   3: Inferred from homology;
KW   Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW   Nucleus.
FT   CHAIN           1..1074
FT                   /note="Topoisomerase 1-associated factor 1"
FT                   /id="PRO_0000410308"
FT   REGION          572..595
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          936..1074
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        579..595
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        960..980
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1011..1034
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1074 AA;  122782 MW;  CF4047A40F036E41 CRC64;
     MELPDSPPPL DAPSPPHFLD APQDRWNVFY PAVQTLVNAL GGYEEVESPP DSGIFETVYR
     PGDSVLGVLK DLKKLWRKDD EDDERTVARC MYRAELMKEL VAIVVECAER GEWGRKVALV
     ACDLIAALTW PIDVAQELKE IEDEGPVVTD YASLLRAQLE YKALFLKTTK PLKSILSLMV
     PCLAKPRKDE KDSRIISLGL HVVRNLLAIK DAVAEGTATG EKEEFAHLQS DLITQLDSLT
     YLQLFLTLAS CADKTDLNPF NVIVLDILHL IFRGIKPSEL VQDQKRVPID SLAKLLEKEK
     KQKALNSRVG STRHSRFGTT ITVKTAEQRV VLHRQTAIIE NPGKILDMTK RKKAVVAKRM
     DDLTVFVNLS SDAMVILQSF SKAFLEICYN TFIESILRDI RMERTKIRPS DNIRVFYLSS
     FFIEYLLLLR HKLLQKGGSR RLEELPLGLV AQIAEMDSVK WLFARLRICW DDKPKAWTEL
     QACIECFTQI LLLIDDMSTS TNEEDVEVAE ILQHQLYYNY DILDSALAVV REYKNQSIAY
     LDSIIHFAYV LLRMLEKYSK TKAFMFIRKR KNTHKKRKER QAASQANADR EQRKIPEEYG
     DEGEEAFAPD EDAPSYAEHA FTFQSFEKRF AQEAVVNTLL TYLERFLEFD GPEPMKRVVG
     LMHRQVIKAH AEGLYFKVST LIIFRRILDK QHVLPAAPSS RDLITLITYI LRKFFKHVEK
     EPFTLVEALS SKSRGKWKTV GGGSDDDDDE MAGQRGRIKE KMGPVELQFI KKHKFSWSQQ
     MSIAFAIIWG DGHGYLIKWI VEVLEQVLAA KQEIVLTTDG GINGDEDEED EDGNARVRRF
     GRPSDEAISK FTQFDLQPEE SEQITAVTSN PHFRLMLKLL SFDLPPPPME LDFEEDVSSE
     ELALAREKSD SAWFLPANVL PSDIEASIGA LKQYMEEPPT LDDDPKKLLR RKARATRRRR
     RSPSVESYDS ETGEVRPGHQ HKKNPRQKRA KKAVETQNYK SAAFIEDSDD EDPEATRRFF
     ENEERLRREM DELAAQGGHP MMERGVKRKR GKKNGKGAIS DTPPPSQRGN DRET