ID   TOF1_CRYNJ              Reviewed;        1074 AA.
AC   P0CR92; Q55QA1; Q5KFW4;
DT   28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Topoisomerase 1-associated factor 1;
GN   Name=TOF1; OrderedLocusNames=CNF00340;
OS   Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS   MYA-565) (Filobasidiella neoformans).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=214684;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JEC21 / ATCC MYA-565;
RX   PubMed=15653466; DOI=10.1126/science.1103773;
RA   Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA   Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA   Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA   Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA   Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA   Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA   Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA   Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA   Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA   Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT   "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT   neoformans.";
RL   Science 307:1321-1324(2005).
CC   -!- FUNCTION: Involved in chromosome segregation during meiosis and DNA
CC       damage repair. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAW44173.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AE017346; AAW44173.1; ALT_SEQ; Genomic_DNA.
DR   RefSeq; XP_571480.1; XM_571480.1.
DR   AlphaFoldDB; P0CR92; -.
DR   SMR; P0CR92; -.
DR   STRING; 5207.AAW44173; -.
DR   PaxDb; P0CR92; -.
DR   EnsemblFungi; AAW44173; AAW44173; CNF00340.
DR   VEuPathDB; FungiDB:CNF00340; -.
DR   eggNOG; KOG1974; Eukaryota.
DR   InParanoid; P0CR92; -.
DR   OrthoDB; 839367at2759; -.
DR   Proteomes; UP000002149; Chromosome 6.
DR   GO; GO:0031298; C:replication fork protection complex; IBA:GO_Central.
DR   GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR   GO; GO:0000076; P:DNA replication checkpoint signaling; IBA:GO_Central.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0043111; P:replication fork arrest; IBA:GO_Central.
DR   GO; GO:0048478; P:replication fork protection; IBA:GO_Central.
DR   InterPro; IPR044998; Timeless.
DR   InterPro; IPR006906; Timeless_N.
DR   PANTHER; PTHR22940; PTHR22940; 1.
DR   Pfam; PF04821; TIMELESS; 1.
PE   3: Inferred from homology;
KW   Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..1074
FT                   /note="Topoisomerase 1-associated factor 1"
FT                   /id="PRO_0000301737"
FT   REGION          572..595
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          936..1074
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        579..595
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        960..980
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1011..1034
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1074 AA;  122827 MW;  4BD986B764BDC664 CRC64;
     MELPDSPPPL DAPSPPHFLD APQDRWNVFY PAVQTLVNAL GGYEEVESPP DSGIFETVYR
     PGDSVLGVLK DLKKLWRKDD EDDERTVARC MYRAELMKEL VAIVVECAER GEWGRKVALV
     ACDLIAALTW PIDVAQELKE IEDEGPVVTD YASLLRAQLE YKALFLKTTK PLKSILSLMV
     PCLAKPRKDE KDSRIISLGL HVVRNLLAIK DAVAEGTATG EKEEFAHLQS DLITQLDSLT
     YLQLFLTLAS CADKTDLNPF NVIVLDILHL IFRGIKPSEL VQDQKRVPID SLARLLEKEK
     KQKALNSRVG STRHSRFGTT ITVKTAEQRV VLHRQTAIIE NPGKILDMTK RKKAVVAKRM
     DDLTVFVNLS SDAMVILQSF SKSFLEICYN TFIESILRDI RMERTKIRPS DNIRVFYLSS
     FFIEYLLLLR HKLLEKGGSR RLEELPLGLV AQIAEMDSVK WLFARLRICW DDKPKAWTEL
     QACIECFTQI LLLIDDMSTS TNEEDVEVAE ILQHQLYYNY DILDSALAVV REYKNQSIAY
     LDSIIHFAYV LLRMLEKYSK TKAFMFIRKR KNTHKKRKER QAASQANADR EQRKIPEEYG
     DEGEEAFAPD EDAPSYAEHA FTFQSFEKRF AQEAVVNTLL TYLERFLEFD GPEPMKRVVG
     LMHRQVIKAH AEGLYFKVST LIIFRRILDK QHVLPAAPSS RDLITLITYI LRKFFKHVEK
     EPFTLVEALS SKSRGKWKTV GGGSDDDDDE MAGQRGRIKE KMGPVELQFI KKHKFSWSQQ
     MSIAFAIIWG DGHGYLIKWI VEVLEQVLAA KQEIVLTTDG GINGDEDEED EDGNARVRRF
     GRPSDEAISK FTQFDLQPEE SEQITAVTSN PHFRLMLKLL SFDLPPPPME LDFEEDVSSE
     ELALAREKSD SAWFLPANVL PSDIEASIGA LKQYMEEPPT LDDDPKKLLR RKARATRRRR
     RSPSVESYDS ETGEVRPGHQ HKKNPRQKRA KKAVETQNYK SAAFIEDSDD EDPEATRRFF
     ENEERLRREM DELAAQGGHP MMERGVKRKR GKKNGKGAIS DTPPPSQRGN DRET