ID   TOF1_KLULA              Reviewed;        1162 AA.
AC   Q6CUU2;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Topoisomerase 1-associated factor 1;
GN   Name=TOF1; OrderedLocusNames=KLLA0C02255g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Forms a fork protection complex (FPC) with CSM3 and which is
CC       required for chromosome segregation during meiosis and DNA damage
CC       repair. FPC coordinates leading and lagging strand synthesis and moves
CC       with the replication fork. FPC stabilizes replication forks in a
CC       configuration that is recognized by replication checkpoint sensors (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC       TOF1 and CSM3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR   EMBL; CR382123; CAH01148.1; -; Genomic_DNA.
DR   RefSeq; XP_452297.1; XM_452297.1.
DR   AlphaFoldDB; Q6CUU2; -.
DR   SMR; Q6CUU2; -.
DR   STRING; 28985.XP_452297.1; -.
DR   EnsemblFungi; CAH01148; CAH01148; KLLA0_C02255g.
DR   GeneID; 2892434; -.
DR   KEGG; kla:KLLA0_C02255g; -.
DR   eggNOG; KOG1974; Eukaryota.
DR   HOGENOM; CLU_008440_0_0_1; -.
DR   InParanoid; Q6CUU2; -.
DR   OMA; WLKLYDE; -.
DR   Proteomes; UP000000598; Chromosome C.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0031298; C:replication fork protection complex; IEA:EnsemblFungi.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0000076; P:DNA replication checkpoint signaling; IEA:EnsemblFungi.
DR   GO; GO:0043570; P:maintenance of DNA repeat elements; IEA:EnsemblFungi.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0007064; P:mitotic sister chromatid cohesion; IEA:EnsemblFungi.
DR   GO; GO:0043111; P:replication fork arrest; IEA:EnsemblFungi.
DR   GO; GO:0048478; P:replication fork protection; IEA:EnsemblFungi.
DR   InterPro; IPR044998; Timeless.
DR   InterPro; IPR006906; Timeless_N.
DR   PANTHER; PTHR22940; PTHR22940; 1.
DR   Pfam; PF04821; TIMELESS; 1.
PE   3: Inferred from homology;
KW   Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..1162
FT                   /note="Topoisomerase 1-associated factor 1"
FT                   /id="PRO_0000301740"
FT   REGION          977..1017
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1127..1162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1136..1162
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1162 AA;  133245 MW;  CAE919C2B95A19EA CRC64;
     MSEPDDVDTV KERLAEDRQN IHIQNEYQDI SYTIIKARIG MLATAIGGPD HSVDSVNPPY
     KVGDDCLACI KDLIRWFKLV DDNQKRWDVE MATAEFKILQ NDLIPILLDW EAKNSAATRK
     SKKTGEDISI FFPNKSYHDR IALGALQLMV LMTWPLIITD QSSYNQVNYY FELKKHQLLY
     KHAILTTENG KVLKAAIRLA LNVMSVDVDH RTARDDSLIR MVLNFLKNVV AIEPGEVTIS
     SVKRLKRPLT MAEMLPTNIT VDDISINSVI TAFDKNKVFG FLLTVASSLS DAVDPNFVSW
     PLLEVMFFLT KDINPIRLFK NQRKSYKLGT DHEITDSNMT TSGKHLSELL AKEHEKKLNV
     IKNTSSRHSR FGGLLSIQTP QNTRLTIASN SVNMRDDAAL QELDSRKKWN KTITMRLDVI
     EGLSSSFFST EGNSIYMSSD NITSIKEFLA DFVDSSFNLL LQNATDSFTS EIQDQLPLHK
     IEYMLFISWF VKFQRSRCIY EIDATPDYVS GALLDECYIL FTKYLRESYE QKNWPVVHAG
     MLLFTEYLEF LLSLDQSWEA DVQAVISKIL SENMLQLLAS LPKSATSHSS QYVKACINLT
     HVVLKTIDKF DENSSLTVES KRKRKVNLNH TAIEKYAKDN DLDYESAFDI LEEQFKQVTI
     NFDKVFRGYL TEPTISTYIR YLQSYKELED KDLIRVLKFF QRVFVKAKEE LFLFRIDFMI
     LCREILSQQG LPTTSELRTH FTKFNEYYLK QLKNKLKKMP SLYINILFPM LHDSQISYYM
     RHGKMKLTEG TPDIVLPSTF INIPDEGSLP KKVLLDMQVG ILVSSLIDDG YEDLIEALLL
     NLQSSFDGLK GKVAEGINSL EEERVKKVAF NATNSEIKRA LYQQPEFRRL LILAGFAIPE
     ANQPACYFIN NKTPTEVQLV IETIQKHRSL PFESEIGKPA SYYLSSHYQE YEDPEEYGSD
     LNNGSYFEDL EIMDKRTEGR ELSKGKAQSK LVQRSKGKRK AIAHHPEENE SDFEANASSS
     TKLPVVSKEY IMDSDDEDPD FGAIFFENET YLRQLLDKHN GSLTETQFSL FAQFCEERVK
     NNGQLRNDYS ALFNETPKSP IFSDVDAETI IIGSASLPSL ANEPIETQVV SSEEYSSHPL
     EEEVTSEQRK RKKPRIESDT EY