ID   TOF1_MAGO7              Reviewed;        1214 AA.
AC   A4R4R3; G4NGY5;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-MAY-2007, sequence version 1.
DT   25-MAY-2022, entry version 70.
DE   RecName: Full=Topoisomerase 1-associated factor 1;
GN   Name=TOF1; ORFNames=MGG_03991;
OS   Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast
OS   fungus) (Pyricularia oryzae).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia.
OX   NCBI_TaxID=242507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=70-15 / ATCC MYA-4617 / FGSC 8958;
RX   PubMed=15846337; DOI=10.1038/nature03449;
RA   Dean R.A., Talbot N.J., Ebbole D.J., Farman M.L., Mitchell T.K.,
RA   Orbach M.J., Thon M.R., Kulkarni R., Xu J.-R., Pan H., Read N.D.,
RA   Lee Y.-H., Carbone I., Brown D., Oh Y.Y., Donofrio N., Jeong J.S.,
RA   Soanes D.M., Djonovic S., Kolomiets E., Rehmeyer C., Li W., Harding M.,
RA   Kim S., Lebrun M.-H., Bohnert H., Coughlan S., Butler J., Calvo S.E.,
RA   Ma L.-J., Nicol R., Purcell S., Nusbaum C., Galagan J.E., Birren B.W.;
RT   "The genome sequence of the rice blast fungus Magnaporthe grisea.";
RL   Nature 434:980-986(2005).
CC   -!- FUNCTION: Forms a fork protection complex (FPC) with CSM3 and which is
CC       required for chromosome segregation during meiosis and DNA damage
CC       repair. FPC coordinates leading and lagging strand synthesis and moves
CC       with the replication fork. FPC stabilizes replication forks in a
CC       configuration that is recognized by replication checkpoint sensors (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC       TOF1 and CSM3. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR   EMBL; CM001236; EHA47495.1; -; Genomic_DNA.
DR   RefSeq; XP_003719862.1; XM_003719814.1.
DR   AlphaFoldDB; A4R4R3; -.
DR   SMR; A4R4R3; -.
DR   STRING; 318829.MGG_03991T0; -.
DR   PRIDE; A4R4R3; -.
DR   EnsemblFungi; MGG_03991T0; MGG_03991T0; MGG_03991.
DR   GeneID; 2677242; -.
DR   KEGG; mgr:MGG_03991; -.
DR   VEuPathDB; FungiDB:MGG_03991; -.
DR   eggNOG; KOG1974; Eukaryota.
DR   HOGENOM; CLU_004390_0_0_1; -.
DR   InParanoid; A4R4R3; -.
DR   OMA; WLKLYDE; -.
DR   OrthoDB; 839367at2759; -.
DR   Proteomes; UP000009058; Chromosome 6.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR044998; Timeless.
DR   InterPro; IPR006906; Timeless_N.
DR   PANTHER; PTHR22940; PTHR22940; 1.
DR   Pfam; PF04821; TIMELESS; 1.
PE   3: Inferred from homology;
KW   Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..1214
FT                   /note="Topoisomerase 1-associated factor 1"
FT                   /id="PRO_0000301742"
FT   REGION          316..357
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          555..601
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          815..841
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          894..1017
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1044..1214
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        322..351
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        899..928
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        929..944
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        969..1017
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1068..1094
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1113..1129
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1175..1196
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1214 AA;  137298 MW;  5F5773B876F1E443 CRC64;
     MEASDGSNDV VHPEVRAHIN SLVSALGGFS ADDDGRYKLG DDALEVLRDM KKWIRFYDEK
     TNRMDVARCL SEANVVGGDL LQILATWPEA QSDSKFKARV ALACYEIMTP LTWPIEKDPE
     RTTVNHYRHM PVLQLAQLGY KRDIINFDGA QILHTAIRVA LPSMAVPIGD RTPRDQGIIK
     LVLYFLRNIA MIAPPPGLKY DGDESQISRS ATIDAFSYQD IFLAILTIAS NMGEDFRTED
     VIIMEIIFHL VKRVDIRKLF LDGEALSKAK GEDLVALIKK EKSMLRAQNK RAPTRHNRFG
     TMVWVQRADG RMSAISGQDA LRDSATRERK MDDSKTFRPP RRSRKAEMEP KDLGSPVSLN
     ERANKQLRGF VEEFLDSGFN PLFQHIRKTI DREAPYLLHQ HRAQFFYLTA WFLQAERERI
     KTAKNQGSST TQDDVSPFNL VAGVLNQEMF ITLSRAMDRA YNDKDWPELT TVMRCFSQIL
     LTVQDMADSG DEENEDIAEN ILNRLFYEEA THDAIANIAR NFKDQGFEYL DACTELIHTY
     LRILESYSKQ NVDMQVRSRK RTRKKKKAAK ATGEDGDPVD DTRIDDGDDS ANDEASAERT
     SRERKFDFGR FAMRFVPQGV VDTFVQFTKY YRDLDDSQLK RAHRYFFRVA FKQDMSVMLF
     RVDIIHLLYN MIKGPEALDK SSSMFKDWEE LVKQIIRKCV RKIEDRPALI IEMLFSKINS
     TAHYLEFGFE KQTISGSANP RPGAELEFKK QDLNLEQQIA IAVGVLLDKN QADHLTWVKN
     VLSNAEVERR AWETAQDALP SVESQNVEGD IENEAPAASS IPQDSTPTIV RPDHDGRRGA
     MFKNPHLRLL MRLVGFERLA PSEAETPESA WIIPANLRAD QLQEAVGHIN AAEFNPPVFD
     DDKSAEDQLR RKSAGTTSRR KKAAFDDDEE NDVDNEDDDG LEEFLFEPGG PTARKSDVRP
     KKKLTRRKRS PGQELTDEQQ DEKARLRREK DLEKQRKIKS AARVHSSDEE SDQEADAAFF
     AREKERMAAR NALYSRLARN PGEFAAEKAA GKTTKVPVLL SDESGGEDSV AAAERSKRRK
     RKSESVAGDE DDRDGGSDGG SEDAGTPARD APETKPLPKR RKKATAFVVS SDEEDSGDEA
     PANTRGQSSQ RSAAEDAMET DETPLSSSPH KTKTTDVEMA NDDDHDEEDT PVKAPTQRRP
     RAKAGFVLDS SDEE