TOF1_NEOFI
ID TOF1_NEOFI Reviewed; 1177 AA.
AC A1D9I5;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Topoisomerase 1-associated factor 1;
GN Name=tof1; ORFNames=NFIA_028970;
OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=331117;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC / WB 181;
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
CC -!- FUNCTION: Forms a fork protection complex (FPC) with csm3 and which is
CC required for chromosome segregation during meiosis and DNA damage
CC repair. FPC coordinates leading and lagging strand synthesis and moves
CC with the replication fork. FPC stabilizes replication forks in a
CC configuration that is recognized by replication checkpoint sensors (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC tof1 and csm3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR EMBL; DS027693; EAW20466.1; -; Genomic_DNA.
DR RefSeq; XP_001262363.1; XM_001262362.1.
DR AlphaFoldDB; A1D9I5; -.
DR SMR; A1D9I5; -.
DR STRING; 36630.CADNFIAP00002719; -.
DR PRIDE; A1D9I5; -.
DR EnsemblFungi; EAW20466; EAW20466; NFIA_028970.
DR GeneID; 4589179; -.
DR KEGG; nfi:NFIA_028970; -.
DR VEuPathDB; FungiDB:NFIA_028970; -.
DR eggNOG; KOG1974; Eukaryota.
DR HOGENOM; CLU_004390_0_0_1; -.
DR OMA; WLKLYDE; -.
DR OrthoDB; 839367at2759; -.
DR Proteomes; UP000006702; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR044998; Timeless.
DR InterPro; IPR006906; Timeless_N.
DR PANTHER; PTHR22940; PTHR22940; 1.
DR Pfam; PF04821; TIMELESS; 1.
PE 3: Inferred from homology;
KW Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..1177
FT /note="Topoisomerase 1-associated factor 1"
FT /id="PRO_0000301743"
FT REGION 575..597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 791..815
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 874..1001
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1021..1177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 800..815
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 885..909
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 962..977
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1021..1047
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1057..1078
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1082..1103
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1116..1135
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1138..1155
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1177 AA; 134953 MW; F98C8138F7947ACC CRC64;
MDEEGAPLSE SVQVVDPDVR AHVYSLVTAL GGFNGESADR YVLGDDALAC LRDIKRWLKL
YDEKNNRMDV ARCLGEANLV NGDLLPILTL WSTSGQKSKH MSRIALACLE LLVPLTWPLE
VPSKMTVNHH RHTPYLQQAQ VLYKRGILSH GSDSILRTII RIGLPSMAVP RSERTTRDEG
ILKLMLYFLR NIAVISPNAR LAAEGDEEET SRSATINAFQ NQDAFALLLT MCSNVGEDFS
LQDVVLLEIL FHIVKGVNVE KLFMNDAQRK AKRTDELGEL LQKESSLRRE YAKNAPTRHG
RFGTMIWVKR DDAKVSTVSG QDVLKDSQTT LHKMDQSKKW NKPQIRRRAA EVTANNDFNT
PVNLNSTATK NLRMFVEEFL DSGFNPLFTH VRKAIEREAD RVLDINTRQF FYTVAWFLEA
ERVRRAHQRE KRHLGDKSLN EIEPDSFALV ASVLNQETFV FLNRSMQYSY DNKDVEDLTA
EMRCFTQILL TVQEMAQSPL EEDQEIADNI QNRIFYEETT HDRIIAIVRG YKDQGFGYLD
ACTELAHVFL KMLEHYSKEN VDMHIRSRRR AKRKAKQAKQ ADIEGDDEEQ ASEEEDLMDA
ERISKERKFD FKRFAAKFCN QSCVDTFIAF TKYYKELNVD QLKRAHRYFY RIAFKQEMSV
LLFRLDIINL FYRMIKGPGA LDSNKPIFKE WEELVRQIIR RMVKKIDQRP ALITELLFSK
INSTVFYLEY GHEKQTISAS KRPPAELEVD PREAKTTDEK IKIVVHVMVK DEHTDLVKWV
SDVLNSAADE REAWESQEQH SEGQKAPNPM IPVKSNNESC QKAMFSNAKL RLLMTLVRFE
RLGMEDVPGA SWIVPSSLTS QDLRHTRSMI EQCLTEPVTE SSDRDLSQLI RRKSGNNTRR
DRDDQTADVD FGSDSEGDDN VPDGPLFPPN PRSRASALEQ LKKQRKKRRK QAGEEEEPDE
EDLEGRRRAR LENALARQAK IKSDLYIHAS DEQTDEEADQ EFFRLEEQRR KEQAERIRKA
LLHGVVEEAS ENSRKKGSGR KRQSDQHTAS TADSQSKRQR RQQRTGGLDD DDDLVMTGTD
ARSPDSLGQG SPSLQGANDV EDTPVTSEEN ELDFDDDLAF SRNRNRDKVL SAENDDSDTE
PPAPDTIDED DDDEAAPVAA PPRRRMRAGF VIESDSE
