TOF1_NEUCR
ID TOF1_NEUCR Reviewed; 1235 AA.
AC Q7S2A9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2003, sequence version 1.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Topoisomerase 1-associated factor 1;
GN Name=tof-1; ORFNames=B11E5.220, NCU09552;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Forms a fork protection complex (FPC) with csm-3 and which is
CC required for chromosome segregation during meiosis and DNA damage
CC repair. FPC coordinates leading and lagging strand synthesis and moves
CC with the replication fork. FPC stabilizes replication forks in a
CC configuration that is recognized by replication checkpoint sensors (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC tof-1 and csm-3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR EMBL; BX842620; CAE76157.1; -; Genomic_DNA.
DR EMBL; CM002240; EAA29535.1; -; Genomic_DNA.
DR RefSeq; XP_958771.1; XM_953678.2.
DR AlphaFoldDB; Q7S2A9; -.
DR SMR; Q7S2A9; -.
DR STRING; 5141.EFNCRP00000007616; -.
DR PRIDE; Q7S2A9; -.
DR EnsemblFungi; EAA29535; EAA29535; NCU09552.
DR GeneID; 3874918; -.
DR KEGG; ncr:NCU09552; -.
DR VEuPathDB; FungiDB:NCU09552; -.
DR HOGENOM; CLU_004390_0_0_1; -.
DR InParanoid; Q7S2A9; -.
DR OMA; WLKLYDE; -.
DR Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR GO; GO:0031298; C:replication fork protection complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0000076; P:DNA replication checkpoint signaling; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0043111; P:replication fork arrest; IBA:GO_Central.
DR GO; GO:0048478; P:replication fork protection; IBA:GO_Central.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR044998; Timeless.
DR InterPro; IPR006906; Timeless_N.
DR PANTHER; PTHR22940; PTHR22940; 1.
DR Pfam; PF04821; TIMELESS; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 3: Inferred from homology;
KW Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..1235
FT /note="Topoisomerase 1-associated factor 1"
FT /id="PRO_0000301744"
FT REGION 327..357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 584..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 812..841
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 897..1235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 908..933
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 934..948
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 987..1043
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1080..1099
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1100..1117
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1126..1146
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1157..1171
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1235 AA; 140517 MW; 433E3344D9F4974E CRC64;
MEAADINDDV VHPEVRAHIT NLVSALGGYS ADDDGSYKLG DEALDVLRDL KKWIRFYDEK
TNRMDVARCL AEANLVGGDL LQILTLWPQS ETDSKYKARI ALACFEVMVP LTWPIEKERA
EMTINHHRHM PVLQLAQLGY KRAIINFDAI PILNTAVRVA LPSMAMPIGE RTPRDQAIIK
LILFFLRNVA MIAPPQGVKC EGDETQVSRS ATIDAFSYQD IFLTLLTLAS NMGEDFRTED
VVIMEIIFHL VKRVDPSSLF VSEKQLNKAK GQELASEMRK EAAMLKSYNK TTTTRHSRFG
TMIWVKRADG KMVTVSGQEA LLDAKTRERK MDNSKTFRPP RRARKPEMEP KDLGPPVTLD
ERARQQLRSF VQDFLDSGFN PLFLHVRQSI DREALHALNQ HKSQFFYLVA WFLEAERMRR
KAKRDESKST SAAGEEVNSF NLVAAVLQQE MFASMNRALD RSYSDKDWQL LTSVMRCYTQ
IFLTVQEMSE SPNEEDQEIA ENTLSRLFYE ETTHDLIANI ARTYKDQGFE YLDAATELVH
TFLRILEGYS KQNVDLQVRS RKRARRKKKA AKAAAAVAAA RAAGEEAEDV GVPEDNDADD
SGDDEQHAER VTQERKFEFG KFAIRFAPQG VVDTFVAFTK FYRDLNDAQL KRAHRYFYRV
AFKLELSIML FRLDIINLFY NMVQGPEPLD KSSPMFKEWE ELSKQIIRKC VKKLQERPAL
FTELLFSKIG STTHFLEHGY EKPVTTTTPR PGAELEFKRA TERDEQIGIA VSVLIDKQQV
EHLQWLKDQL TSAMSERQAW ENVDKAMAAT TEGAADGEAA DERSNKSAPP HITIRPDTEA
RRTAMFKNPH LRLLMRLVGM ERLTPTLDET PDSTWILPGS HTAEAIQDTI DLINKAEFSP
PTFEDGGSAE DQLRRKSAAA SRRTRAAYDD DEEEIRGFLG DDDDEDFLFA PGGPTARKPD
ARPQKKRQRK RRREAGSGDE EDEGVSDEVL AARAKKRREK ELEKIRKIKS EMYVHASDDE
TDDERDREFF ERERKRQETK DSKFDSMLGA LGLSVLSQVN GGEKSAWEAV LDDEPESDES
ENEGRKNAKR RKKQVASGSE EEQEEEEEEE EEEDSDEELP TKQAKSKTSK RKAAVPSKRP
ARRPGTAKKR AVVELSDNDE DEDEEEDAMD VDSANERTTR NEAPLPSSPG EGLGRRIDKM
AMDDGDEDED DQPVVAARQR PKARGGFIID SSDEE
