TOF1_PICST
ID TOF1_PICST Reviewed; 1256 AA.
AC A3LR41;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 25-MAY-2022, entry version 54.
DE RecName: Full=Topoisomerase 1-associated factor 1;
GN Name=TOF1; ORFNames=PICST_88189;
OS Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS Y-11545) (Yeast) (Pichia stipitis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX NCBI_TaxID=322104;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX PubMed=17334359; DOI=10.1038/nbt1290;
RA Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA Passoth V., Richardson P.M.;
RT "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT yeast Pichia stipitis.";
RL Nat. Biotechnol. 25:319-326(2007).
CC -!- FUNCTION: Forms a fork protection complex (FPC) with CSM3 and which is
CC required for chromosome segregation during meiosis and DNA damage
CC repair. FPC coordinates leading and lagging strand synthesis and moves
CC with the replication fork. FPC stabilizes replication forks in a
CC configuration that is recognized by replication checkpoint sensors (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC TOF1 and CSM3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR EMBL; CP000497; ABN65319.2; -; Genomic_DNA.
DR RefSeq; XP_001383348.2; XM_001383311.1.
DR AlphaFoldDB; A3LR41; -.
DR SMR; A3LR41; -.
DR STRING; 4924.XP_001383348.2; -.
DR PRIDE; A3LR41; -.
DR EnsemblFungi; ABN65319; ABN65319; PICST_88189.
DR GeneID; 4838311; -.
DR KEGG; pic:PICST_88189; -.
DR eggNOG; KOG1974; Eukaryota.
DR HOGENOM; CLU_008440_0_0_1; -.
DR InParanoid; A3LR41; -.
DR OMA; WLKLYDE; -.
DR OrthoDB; 839367at2759; -.
DR Proteomes; UP000002258; Chromosome 3.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0008156; P:negative regulation of DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR044998; Timeless.
DR InterPro; IPR006906; Timeless_N.
DR PANTHER; PTHR22940; PTHR22940; 1.
DR Pfam; PF04821; TIMELESS; 1.
PE 3: Inferred from homology;
KW Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..1256
FT /note="Topoisomerase 1-associated factor 1"
FT /id="PRO_0000301747"
FT REGION 26..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 695..725
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1052..1121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1176..1256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..58
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1063..1082
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1086..1110
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1180..1195
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1202..1219
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1237..1256
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1256 AA; 143745 MW; 6D29487D04DFC3AE CRC64;
MSVSDIETER TRYYDAYDAD DAELDGFIVS DEENDNLENE NRNERDPDSR NQDDVDEYGQ
PQIDRIRTMT EALAPNESQA SKVLRAHISV LVSALGGPDH TSNISPPPYK LGHDALACLK
DIKRWIKSVD DRQDSYQVAL ACAESGLVIN DLIVILCQWD SKNKKKEDFR NKRTMEKIML
ACLELLVLLT WSVELRPESS DKQKLLYYDV KKAQIKYKRA ILTYNKGQTL KAVIRLVLPI
ISKERVDREP KDNAIMRLVL FFFRNILYVE PPSPSISKKS SKTIVVTDNM PEGVSYDDIS
LSATISAFSK NRVLMLFLTL SSGIGVDFDS RFLGPTLLEC IHLLVRGVDP NDILKLKQMR
IPTEINDPGN SSSPFHNVPP ASSTTGLQLQ DLLAKESKIK NSQTQSMSTR HGRFGSLLSI
RGDHSMSFVV SGQEALINAG QTMQKLDRSK KWKNRSYFKY DSDEYTKSSN TTYMNYGGLV
ILHEFIESFL AGGCFNILIE KLSSVFSSSD SILEKEYETA TFFLTIAWFF QYKREKTVLY
SSGTTNLQPI GEEDDRNDFG SVGAALSQVN FILLVKYCVD SFSISPKRWS SLHVVLICLK
ELLEISNTLF TRSSSSAGDE EQNELDRELA EGIISHLLVT QDFLSILYHL PQTASRHSPE
YLKVCISVVH ILLKTLKNFA EEDVKLFIQT TRRNSKKKAN KESNDQSDAV EQVDESDTED
KRTHARVTRE RKINYERTEV KFFHQDTVST YIEYLSRYED LTHYEIKKCL TYFHRLFVVR
KDFNGLYRLD FMQVLHKLRD YLPSQSNIRG QVDEFIYYFM KKFKASIERF PNPIEILFPR
FEDAESKTYL ATGELYIQTE REIRSANVKR FEPGKPLEFV RHFEDNEKYK ILVSALYEQG
LSNMLVCLVE DLQRIHGIKQ LDEDVDEVLQ LKEDFRQYVL TNSHLRLLLR TVGLIPGYSL
SQECQVPSKL TSSDIDSAIT LIKKWMGLQP ATFEDGKDPS YFLRSTGDTV DRPIDYSDSE
DDIAFEITPK DRPNESHYDM LTELDELERA ISGAENREKG KARKRGNKSS STIKKKSLQS
RSRRPPRFNV DSDDESELRK EVKSSEFVHD SDDESDDEAF FEREERLRQM LNSSGGIVNA
QQLSEFKKVW ASLESTGGIA TASQVVRAVE SVANISDGVD THSHQDDKSQ PSDSENEDSS
EEVSEVQIRK RLRIEDDEND SDNNVSENYV SAEEGEEATP TVKRKKRLVI SDDEDE
