TOF1_YARLI
ID TOF1_YARLI Reviewed; 1054 AA.
AC Q6CFJ2;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 71.
DE RecName: Full=Topoisomerase 1-associated factor 1;
GN Name=TOF1; OrderedLocusNames=YALI0B06512g;
OS Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Dipodascaceae; Yarrowia.
OX NCBI_TaxID=284591;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CLIB 122 / E 150;
RX PubMed=15229592; DOI=10.1038/nature02579;
RA Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA Weissenbach J., Wincker P., Souciet J.-L.;
RT "Genome evolution in yeasts.";
RL Nature 430:35-44(2004).
CC -!- FUNCTION: Forms a fork protection complex (FPC) with CSM3 and which is
CC required for chromosome segregation during meiosis and DNA damage
CC repair. FPC coordinates leading and lagging strand synthesis and moves
CC with the replication fork. FPC stabilizes replication forks in a
CC configuration that is recognized by replication checkpoint sensors (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the fork protection complex (FPC) consisting of
CC TOF1 and CSM3. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the timeless family. {ECO:0000305}.
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DR EMBL; CR382128; CAG82801.1; -; Genomic_DNA.
DR RefSeq; XP_500570.1; XM_500570.1.
DR AlphaFoldDB; Q6CFJ2; -.
DR SMR; Q6CFJ2; -.
DR STRING; 4952.CAG82801; -.
DR PRIDE; Q6CFJ2; -.
DR EnsemblFungi; CAG82801; CAG82801; YALI0_B06512g.
DR GeneID; 2907602; -.
DR KEGG; yli:YALI0B06512g; -.
DR VEuPathDB; FungiDB:YALI0_B06512g; -.
DR HOGENOM; CLU_004390_0_0_1; -.
DR InParanoid; Q6CFJ2; -.
DR OMA; MHRIAIK; -.
DR Proteomes; UP000001300; Chromosome B.
DR GO; GO:0031298; C:replication fork protection complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0000076; P:DNA replication checkpoint signaling; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0043111; P:replication fork arrest; IBA:GO_Central.
DR GO; GO:0048478; P:replication fork protection; IBA:GO_Central.
DR InterPro; IPR044998; Timeless.
DR InterPro; IPR006906; Timeless_N.
DR PANTHER; PTHR22940; PTHR22940; 1.
DR Pfam; PF04821; TIMELESS; 1.
PE 3: Inferred from homology;
KW Cell cycle; DNA damage; DNA repair; DNA replication inhibitor; Meiosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..1054
FT /note="Topoisomerase 1-associated factor 1"
FT /id="PRO_0000301749"
FT REGION 522..543
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 823..846
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 865..953
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 968..1054
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 823..837
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 888..902
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 924..953
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 968..1027
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1054 AA; 120710 MW; 1A239A67C38E6A19 CRC64;
MEEVDRLVTA SITSIVSALG GCDLASEDAS YVLGEEALAC LKDVKQWLRA FDEKLGRSDV
AKCIASTTLV VSDIPSILST WNTQSEAGQA SKKMDRVALA CLEILVPLLW PIEINDETPD
NVVASADLLR QAQIRYKRAL LGHPSKSILK AVVRLCIPSV AKSKADRDQR DIGIMKLVVF
FIRNMLAIDP MEANGKTDDI NRSAILEAFE NQGVLDLMLT LGSCTGYDIV GVDLPLLDSL
YQMVKGLDVV EIFETSPNEP DNQLKNLLDT ERRNKPVGSS RHSRFASTMT VLHNGSKISV
TGPTASIEKS LEKFDKSKQP GRRLTKPTTG VWEVPVFVGH SAKRYIRVFS ELFNDSAFNP
LMQTVRKALE QEDDIGPAFK HYLVVLKWFL GVEMHRKTPD FGLIASVVNQ EAFIIIMRSI
RQGVQAKNWT MVKTAMDSFK TTLVVVNLMS DEEVASNIKA RLFYEEEYLT MLADLTRRWA
LPLTFLQSAV DMTHTLIKTL ESFTKANTTL YVRRRRLRAQ KKADDTGNVE DLESLPGEEH
ESAIESRERK FNFSSFESRY FHEDTFTTYR MVLSSFQQLD NCYLGWCLKF LDRAFNKRKC
RVMLFRLDYL QLFRTMVKEL NSSNPWRKPF EHFFKKYMRQ MIPMMKERPC LMVEVIFAKI
PGTLHYLESG EEKPVRELKE RTSKFVYEFT EGDDIPEERK VCILVASLLD EEKKQLVEWF
IDELDSLLRA RTGDTQVLNP PKATDELNVP KSIDEDDKFR LLMELVGFTL SRIPKSSVGC
KTLCSLPGEV SRADIERATG WLKQWYSTPV DFEPFNKIKR VRRDDKERRG AGGDKEGSQD
AEEDDELPLF LASDSEDDIE NINDEMFAPV PKEKQQKTKQ KLKRKGTSKS TREREKQRDY
TAKTTIDIPM SYKSADFIGA SDDDSDDERD RKFFEKENEL REELRKAEPT INLEEKVDKA
FKRQYAYVSD GEEEPLSETE SHKQYREKVD EDRDDLSHMA ERENRQYDFG DEGHDSDNLE
IIDDDSKSPV AAEGTTSSQP IRKRRIVDDS DDDE
