TOF2_YEAST
ID TOF2_YEAST Reviewed; 771 AA.
AC Q02208; D6VX76;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Topoisomerase 1-associated factor 2;
GN Name=TOF2; OrderedLocusNames=YKR010C; ORFNames=YK109;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=1441752; DOI=10.1002/yea.320080908;
RA Duesterhoeft A., Philippsen P.;
RT "DNA sequencing and analysis of a 24.7 kb segment encompassing centromere
RT CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open
RT reading frames.";
RL Yeast 8:749-759(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP IDENTIFICATION, AND INTERACTION WITH HPR1.
RX PubMed=10028183;
RX DOI=10.1002/(sici)1097-0061(19990115)15:1<35::aid-yea340>3.0.co;2-r;
RA Park H., Sternglanz R.;
RT "Identification and characterization of the genes for two topoisomerase I-
RT interacting proteins from Saccharomyces cerevisiae.";
RL Yeast 15:35-41(1999).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-397 AND THR-405, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBUNIT: Interacts with HPR1. {ECO:0000269|PubMed:10028183}.
CC -!- INTERACTION:
CC Q02208; Q00684: CDC14; NbExp=8; IntAct=EBI-27048, EBI-4192;
CC Q02208; P25651: CSM1; NbExp=4; IntAct=EBI-27048, EBI-22001;
CC Q02208; P47035: NET1; NbExp=6; IntAct=EBI-27048, EBI-25953;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 339 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: To yeast YJL076w. {ECO:0000305}.
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DR EMBL; X65124; CAA46242.1; -; Genomic_DNA.
DR EMBL; Z28235; CAA82080.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09166.1; -; Genomic_DNA.
DR PIR; S25814; S25814.
DR RefSeq; NP_012935.3; NM_001179800.3.
DR PDB; 5HOI; X-ray; 3.30 A; D/E/F=497-517.
DR PDB; 5V3N; X-ray; 1.30 A; B=384-398.
DR PDBsum; 5HOI; -.
DR PDBsum; 5V3N; -.
DR AlphaFoldDB; Q02208; -.
DR SMR; Q02208; -.
DR BioGRID; 34143; 155.
DR DIP; DIP-3803N; -.
DR IntAct; Q02208; 18.
DR MINT; Q02208; -.
DR STRING; 4932.YKR010C; -.
DR iPTMnet; Q02208; -.
DR MaxQB; Q02208; -.
DR PaxDb; Q02208; -.
DR PRIDE; Q02208; -.
DR EnsemblFungi; YKR010C_mRNA; YKR010C; YKR010C.
DR GeneID; 853880; -.
DR KEGG; sce:YKR010C; -.
DR SGD; S000001718; TOF2.
DR VEuPathDB; FungiDB:YKR010C; -.
DR eggNOG; ENOG502QW4V; Eukaryota.
DR GeneTree; ENSGT00940000176631; -.
DR HOGENOM; CLU_362533_0_0_1; -.
DR InParanoid; Q02208; -.
DR OMA; DIGENCR; -.
DR BioCyc; YEAST:G3O-31987-MON; -.
DR PRO; PR:Q02208; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; Q02208; protein.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0019211; F:phosphatase activator activity; IDA:SGD.
DR GO; GO:0000182; F:rDNA binding; IBA:GO_Central.
DR GO; GO:0031030; P:negative regulation of septation initiation signaling; IBA:GO_Central.
DR GO; GO:0007000; P:nucleolus organization; IMP:SGD.
DR GO; GO:0034503; P:protein localization to nucleolar rDNA repeats; IMP:SGD.
DR GO; GO:0070550; P:rDNA chromatin condensation; IMP:SGD.
DR GO; GO:0000183; P:rDNA heterochromatin assembly; IMP:SGD.
DR InterPro; IPR018844; Dnt1-like_N.
DR InterPro; IPR043185; Dnt1/Tof2/Net1.
DR PANTHER; PTHR28196; PTHR28196; 2.
DR Pfam; PF10407; Cytokin_check_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..771
FT /note="Topoisomerase 1-associated factor 2"
FT /id="PRO_0000072621"
FT REGION 48..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 346..367
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 633..771
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..295
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 296..316
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 346..363
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 637..677
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 686..708
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 725..739
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 749..765
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 397
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 405
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT HELIX 503..512
FT /evidence="ECO:0007829|PDB:5HOI"
SQ SEQUENCE 771 AA; 86366 MW; DDA5E96B833575A3 CRC64;
MIKMWRLQIV LVPPSAQDII TFLEARLNTP QSVSPMVQYN EDIITHNNSI NNCSDPSPTS
PSSQNSIQSN RSSDFINYLP NCKKFLHFTD GDNTLLQLSN EILTKFDRLY PNFKESIEIV
SLQDRHGCDL DSEFIIKDVF ENDGVVLVIL KDELDWSRNQ HISLLQLARQ RRRQDNKPST
KSIVTEKRKK ISKEDLSSIS NKDTMHLIAK SSLKNNFINK SRVSTPLMNE ILPLASKYDA
LNKEKCPMPL TSTVVASNVH KDVKDHARAK EGVVTQGSDN NKENIPSSTQ QQKNDGAKRA
ESKDLDLLRN SSEDADYEPA DENSPQISFD SIDTDFQLST TSHTNSDMHI QYSNPSSGAH
SPRKSSLEIK VQNKKGDDLP LNDKDIGENC RRIEAFSDEE DFNETDNDRA DSFINNSKKA
SMGFRDINSD LDSVSFNSDI ENAVQSTQST KNVVSPPFFP EKELNNRLHQ SQGKEALFRL
VEKEFPDKSL GAASSTSHAK DVKIQETIRK LNRFKPTGET KVQKRNSITE PYYGKFGIMK
KDKPKSITSK GVSLETKHFD DPNTIISGEK FAKFGKIKVK RKTDDVGSKV IEFKRKRNMG
NRSLKDIFAN AGKPPNAAST IKVVKLMRDP VDNSKDKVEA TSNSTAQEQE QVSPKLPVMN
STPGKRKNGQ AIPSSLERTP QLKKVKVTRS HSSPSSSSSM SLESSLDSSS SDDSDDDSDS
RNVQVKKINF KTSHGPAGNS NGKPMLDVDD NEINTKKYQT PKYVESDEDD Q
