ID   TOG1A_AGEAP             Reviewed;         112 AA.
AC   P15969;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Omega-agatoxin-1A;
DE   AltName: Full=Omega-agatoxin IA;
DE            Short=Omega-Aga-IA;
DE   Contains:
DE     RecName: Full=Omega-agatoxin-1A major chain;
DE   Contains:
DE     RecName: Full=Omega-agatoxin-1A minor chain;
DE   Flags: Precursor;
OS   Agelenopsis aperta (North American funnel-web spider) (Agelenopsis
OS   gertschi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Agelenopsis.
OX   NCBI_TaxID=6908;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, SUBCELLULAR LOCATION,
RP   AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=1383207; DOI=10.1016/s0021-9258(19)36742-0;
RA   Santos A.D., Imperial J.S., Chaudhary T., Beavis R.C., Chait B.T.,
RA   Hunsperger J.P., Olivera B.M., Adams M.E., Hillyard D.R.;
RT   "Heterodimeric structure of the spider toxin omega-agatoxin IA revealed by
RT   precursor analysis and mass spectrometry.";
RL   J. Biol. Chem. 267:20701-20705(1992).
RN   [2]
RP   PROTEIN SEQUENCE OF 37-102, SUBCELLULAR LOCATION, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=2295621; DOI=10.1016/s0021-9258(19)40129-4;
RA   Adams M.E., Bindokas V.P., Hasegawa L., Venema V.J.;
RT   "Omega-agatoxins: novel calcium channel antagonists of two subtypes from
RT   funnel web spider (Agelenopsis aperta) venom.";
RL   J. Biol. Chem. 265:861-867(1990).
RN   [3]
RP   FUNCTION.
RX   PubMed=1685511; DOI=10.1152/jn.1991.66.2.590;
RA   Bindokas V.P., Venema V.J., Adams M.E.;
RT   "Differential antagonism of transmitter release by subtypes of omega-
RT   agatoxins.";
RL   J. Neurophysiol. 66:590-601(1991).
CC   -!- FUNCTION: Omega-agatoxins are antagonists of voltage-gated calcium
CC       channels. They block insect neuromuscular transmission presynaptically.
CC       This toxin is a blocker of L-type calcium channels (Cav/CACNA1).
CC       {ECO:0000269|PubMed:1685511}.
CC   -!- SUBUNIT: Heterodimer of two subunits, a major chain and a minor chain,
CC       linked by a disulfide bond.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1383207,
CC       ECO:0000269|PubMed:2295621}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:1383207, ECO:0000305|PubMed:2295621}.
CC   -!- PTM: Proteolytically processed to yield the major and the minor chains.
CC   -!- MASS SPECTROMETRY: Mass=7791.4; Method=Plasma desorption; Note=The
CC       measured ranges are 37-102, 110-112.;
CC       Evidence={ECO:0000269|PubMed:1383207};
CC   -!- SIMILARITY: Belongs to the neurotoxin 04 (omega-agtx) family. 01 (type
CC       I omega-agtx) subfamily. {ECO:0000305}.
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DR   EMBL; M95540; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; A45069; A45069.
DR   AlphaFoldDB; P15969; -.
DR   ArachnoServer; AS000175; omega-agatoxin-Aa1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR013605; Toxin_34.
DR   Pfam; PF08396; Toxin_34; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Neurotoxin; Presynaptic neurotoxin; Secreted;
KW   Signal; Toxin; Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..36
FT                   /evidence="ECO:0000269|PubMed:2295621"
FT                   /id="PRO_0000035486"
FT   CHAIN           37..102
FT                   /note="Omega-agatoxin-1A major chain"
FT                   /evidence="ECO:0000269|PubMed:2295621"
FT                   /id="PRO_0000035487"
FT   PROPEP          103..109
FT                   /note="Glu-rich"
FT                   /id="PRO_0000035488"
FT   CHAIN           110..112
FT                   /note="Omega-agatoxin-1A minor chain"
FT                   /id="PRO_0000035489"
FT   CONFLICT        101
FT                   /note="D -> N (in Ref. 2; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   112 AA;  12808 MW;  ACC0ED776E49DF23 CRC64;
     MMKFVVFLAC LFVAAHSFAV EGEEEYFEAE VPELERAKAL PPGSVCDGNE SDCKCYGKWH
     KCRCPWKWHF TGEGPCTCEK GMKHTCITKL HCPNKAEWGL DWRSEESERS PC