ID   TOG3A_AGEAP             Reviewed;          76 AA.
AC   P33034;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Omega-agatoxin-Aa3a;
DE            Short=Omega-AGTX-Aa3a;
DE   AltName: Full=Omega-agatoxin IIIA;
DE            Short=Omega-Aga-IIIA;
DE   AltName: Full=Omega-agatoxin-3A;
OS   Agelenopsis aperta (North American funnel-web spider) (Agelenopsis
OS   gertschi).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Araneomorphae; Entelegynae; Agelenidae; Agelenopsis.
OX   NCBI_TaxID=6908;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Venom;
RX   PubMed=1310319; DOI=10.1016/s0021-9258(18)45925-x;
RA   Venema V.J., Swiderek K.M., Lee T.D., Hathaway G.M., Adams M.E.;
RT   "Antagonism of synaptosomal calcium channels by subtypes of omega-
RT   agatoxins.";
RL   J. Biol. Chem. 267:2610-2615(1992).
CC   -!- FUNCTION: Omega-agatoxin are antagonist of voltage-gated calcium
CC       channels. They block insect neuromuscular transmission presynaptically.
CC       Potent blocker of N- (Cav2.2/CACNA1B) and L-type (Cav1/CACNA1) calcium
CC       channels.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 04 (omega-agtx) family. 03 (type
CC       II/III omega-agtx) subfamily. {ECO:0000305}.
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DR   PIR; A42335; A42335.
DR   PIR; A54252; A54252.
DR   AlphaFoldDB; P33034; -.
DR   ArachnoServer; AS000178; omega-agatoxin-Aa3a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR005853; Omega-agatoxin_II/III_CS.
DR   InterPro; IPR013605; Toxin_34.
DR   Pfam; PF08396; Toxin_34; 1.
DR   PROSITE; PS60023; OMEGA_AGA_II_III; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Presynaptic neurotoxin;
KW   Secreted; Toxin; Voltage-gated calcium channel impairing toxin.
FT   CHAIN           1..76
FT                   /note="Omega-agatoxin-Aa3a"
FT                   /id="PRO_0000087606"
FT   DISULFID        2..19
FT                   /evidence="ECO:0000305"
FT   DISULFID        9..25
FT                   /evidence="ECO:0000305"
FT   DISULFID        16..52
FT                   /evidence="ECO:0000305"
FT   DISULFID        18..40
FT                   /evidence="ECO:0000305"
FT   DISULFID        27..38
FT                   /evidence="ECO:0000305"
FT   DISULFID        59..67
FT                   /evidence="ECO:0000305"
FT   VARIANT         58
FT                   /note="Q -> T"
SQ   SEQUENCE   76 AA;  8518 MW;  6A715CAC9591888B CRC64;
     SCIDIGGDCD GEKDDCQCCR RNGYCSCYSL FGYLKSGCKC VVGTSAEFQG ICRRKARQCY
     NSDPDKCESH NKPKRR