TOL4_ARATH
ID TOL4_ARATH Reviewed; 446 AA.
AC Q6NQK0; O49283;
DT 05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=TOM1-like protein 4 {ECO:0000305};
GN Name=TOL4 {ECO:0000303|PubMed:24316203};
GN Synonyms=TOM1C {ECO:0000303|PubMed:22639582};
GN OrderedLocusNames=At1g76970 {ECO:0000312|Araport:AT1G76970};
GN ORFNames=F22K20.7 {ECO:0000312|EMBL:AEE35914.1},
GN F22K20_7 {ECO:0000312|EMBL:AAC00635.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND REVIEW.
RX PubMed=16488176; DOI=10.1016/j.tplants.2006.01.008;
RA Winter V., Hauser M.-T.;
RT "Exploring the ESCRTing machinery in eukaryotes.";
RL Trends Plant Sci. 11:115-123(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-291, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [8]
RP GENE FAMILY, NOMENCLATURE, AND SUBCELLULAR LOCATION.
RX PubMed=22639582; DOI=10.3389/fpls.2011.00020;
RA Richardson L.G., Howard A.S., Khuu N., Gidda S.K., McCartney A.,
RA Morphy B.J., Mullen R.T.;
RT "Protein-protein interaction network and subcellular localization of the
RT Arabidopsis thaliana ESCRT machinery.";
RL Front. Plant Sci. 2:20-20(2011).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24316203; DOI=10.1016/j.cub.2013.10.036;
RA Korbei B., Moulinier-Anzola J., De-Araujo L., Lucyshyn D., Retzer K.,
RA Khan M.A., Luschnig C.;
RT "Arabidopsis TOL proteins act as gatekeepers for vacuolar sorting of PIN2
RT plasma membrane protein.";
RL Curr. Biol. 23:2500-2505(2013).
RN [10]
RP TISSUE SPECIFICITY.
RX PubMed=24699223; DOI=10.4161/psb.28667;
RA Moulinier-Anzola J., De-Araujo L., Korbei B.;
RT "Expression of Arabidopsis TOL genes.";
RL Plant Signal. Behav. 9:E28667-E28667(2014).
CC -!- FUNCTION: Might contribute to the loading of the ESCRT machinery.
CC {ECO:0000305|PubMed:16488176}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22639582}. Membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=Additional isoforms seem to exist. {ECO:0000305};
CC Name=1;
CC IsoId=Q6NQK0-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:24699223}.
CC -!- SIMILARITY: Belongs to the TOM1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC00635.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC002291; AAC00635.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35914.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58921.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58923.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58925.1; -; Genomic_DNA.
DR EMBL; BT010453; AAQ62873.1; -; mRNA.
DR EMBL; AK221351; BAD94203.1; -; mRNA.
DR PIR; F96798; F96798.
DR RefSeq; NP_001321321.1; NM_001334752.1. [Q6NQK0-1]
DR RefSeq; NP_001321323.1; NM_001334755.1. [Q6NQK0-1]
DR RefSeq; NP_001321325.1; NM_001334751.1. [Q6NQK0-1]
DR RefSeq; NP_177823.2; NM_106348.4. [Q6NQK0-1]
DR AlphaFoldDB; Q6NQK0; -.
DR SMR; Q6NQK0; -.
DR STRING; 3702.AT1G76970.1; -.
DR iPTMnet; Q6NQK0; -.
DR PaxDb; Q6NQK0; -.
DR PRIDE; Q6NQK0; -.
DR ProteomicsDB; 234453; -. [Q6NQK0-1]
DR EnsemblPlants; AT1G76970.1; AT1G76970.1; AT1G76970. [Q6NQK0-1]
DR EnsemblPlants; AT1G76970.2; AT1G76970.2; AT1G76970. [Q6NQK0-1]
DR EnsemblPlants; AT1G76970.3; AT1G76970.3; AT1G76970. [Q6NQK0-1]
DR EnsemblPlants; AT1G76970.6; AT1G76970.6; AT1G76970. [Q6NQK0-1]
DR GeneID; 844033; -.
DR Gramene; AT1G76970.1; AT1G76970.1; AT1G76970. [Q6NQK0-1]
DR Gramene; AT1G76970.2; AT1G76970.2; AT1G76970. [Q6NQK0-1]
DR Gramene; AT1G76970.3; AT1G76970.3; AT1G76970. [Q6NQK0-1]
DR Gramene; AT1G76970.6; AT1G76970.6; AT1G76970. [Q6NQK0-1]
DR KEGG; ath:AT1G76970; -.
DR Araport; AT1G76970; -.
DR TAIR; locus:2025297; AT1G76970.
DR eggNOG; KOG1087; Eukaryota.
DR HOGENOM; CLU_026748_2_1_1; -.
DR InParanoid; Q6NQK0; -.
DR OMA; ANINHDV; -.
DR OrthoDB; 508632at2759; -.
DR PhylomeDB; Q6NQK0; -.
DR PRO; PR:Q6NQK0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q6NQK0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
DR GO; GO:0043130; F:ubiquitin binding; IEA:InterPro.
DR GO; GO:0043328; P:protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; IEA:InterPro.
DR Gene3D; 1.20.58.160; -; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR008942; ENTH_VHS.
DR InterPro; IPR004152; GAT_dom.
DR InterPro; IPR038425; GAT_sf.
DR InterPro; IPR044836; TOL_plant.
DR InterPro; IPR014645; TOM1.
DR InterPro; IPR002014; VHS_dom.
DR PANTHER; PTHR45898; PTHR45898; 1.
DR Pfam; PF03127; GAT; 1.
DR Pfam; PF00790; VHS; 1.
DR PIRSF; PIRSF036948; TOM1; 1.
DR SMART; SM00288; VHS; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
DR PROSITE; PS50909; GAT; 1.
DR PROSITE; PS50179; VHS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..446
FT /note="TOM1-like protein 4"
FT /id="PRO_0000440679"
FT DOMAIN 12..141
FT /note="VHS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00218"
FT DOMAIN 172..260
FT /note="GAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00373"
FT REGION 260..423
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 350..411
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 291
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19245862,
FT ECO:0007744|PubMed:19376835"
FT MOD_RES 366
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9LPL6"
SQ SEQUENCE 446 AA; 49255 MW; 4C335FAB67F551A8 CRC64;
MANDAAACAE RATNDMLIGP DWAINIELCD LINMDPSQAK EAVKVLKKRL GSKNSKVQIL
ALYALETLSK NCGENVYQLI IDRGLLNDMV KIVKKKPELN VREKILTLLD TWQEAFGGRG
GRYPQYYNAY NDLRSAGIEF PPRTESSLSF FTPPQTQPDE DAAIQASLQG DDASSLSLEE
IQSAEGSVDV LMDMLGAHDP GNPESLKEEV IVDLVEQCRT YQRRVMTLVN TTTDEELLCQ
GLALNDNLQH VLQRHDDIAN VGSVPSNGRN TRAPPPVQIV DINHDDEDDE SDDEFARLAH
RSSTPTRRPV HGSDSGMVDI LSGDVYKPQG NSSSQGVKKP PPPPPHTSSS SSSPVFDDAS
PQQSKSSEVI RNLPPPPSRH NQRQQFFEHH HSSSGSDSSY EGQTRNLSLT SSEPQKEEKP
EDLLFKDLVE FAKTRSSKAN NNNRSL
