TOLA_PSEAE
ID TOLA_PSEAE Reviewed; 347 AA.
AC P50600;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Tol-Pal system protein TolA {ECO:0000305};
GN Name=tolA; OrderedLocusNames=PA0971;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PAO;
RX PubMed=8955385; DOI=10.1128/jb.178.24.7059-7068.1996;
RA Dennis J.J., Lafontaine E.R., Sokol P.A.;
RT "Identification and characterization of the tolQRA genes of Pseudomonas
RT aeruginosa.";
RL J. Bacteriol. 178:7059-7068(1996).
RN [2]
RP SEQUENCE REVISION TO N-TERMINUS.
RA Duan K., Sokol P.A.;
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Part of the Tol-Pal system, which plays a role in outer
CC membrane invagination during cell division and is important for
CC maintaining outer membrane integrity. {ECO:0000250|UniProtKB:P19934}.
CC -!- SUBUNIT: The Tol-Pal system is composed of five core proteins: the
CC inner membrane proteins TolA, TolQ and TolR, the periplasmic protein
CC TolB and the outer membrane protein Pal. They form a network linking
CC the inner and outer membranes and the peptidoglycan layer.
CC {ECO:0000250|UniProtKB:P19934}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P19934}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P19934}.
CC -!- SIMILARITY: Belongs to the TolA family. {ECO:0000305}.
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DR EMBL; U39558; AAC44660.2; -; Genomic_DNA.
DR EMBL; AE004091; AAG04360.1; -; Genomic_DNA.
DR PIR; E83525; E83525.
DR RefSeq; NP_249662.1; NC_002516.2.
DR RefSeq; WP_003112574.1; NZ_QZGE01000007.1.
DR PDB; 1LR0; X-ray; 1.91 A; A=226-347.
DR PDB; 6S3W; NMR; -; A=226-347.
DR PDBsum; 1LR0; -.
DR PDBsum; 6S3W; -.
DR AlphaFoldDB; P50600; -.
DR BMRB; P50600; -.
DR SMR; P50600; -.
DR STRING; 287.DR97_966; -.
DR PaxDb; P50600; -.
DR PRIDE; P50600; -.
DR EnsemblBacteria; AAG04360; AAG04360; PA0971.
DR GeneID; 882033; -.
DR KEGG; pae:PA0971; -.
DR PATRIC; fig|208964.12.peg.1009; -.
DR PseudoCAP; PA0971; -.
DR HOGENOM; CLU_038804_1_0_6; -.
DR OMA; PYRSFKM; -.
DR PhylomeDB; P50600; -.
DR BioCyc; PAER208964:G1FZ6-992-MON; -.
DR EvolutionaryTrace; P50600; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019534; F:toxin transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0043213; P:bacteriocin transport; IEA:InterPro.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR InterPro; IPR014161; Tol-Pal_TolA.
DR InterPro; IPR006260; TonB/TolA_C.
DR TIGRFAMs; TIGR02794; tolA_full; 1.
DR TIGRFAMs; TIGR01352; tonB_Cterm; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Cell inner membrane;
KW Cell membrane; Membrane; Reference proteome; Repeat; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..347
FT /note="Tol-Pal system protein TolA"
FT /id="PRO_0000072624"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P19934"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..347
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P19934"
FT REGION 112..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 228..265
FT /evidence="ECO:0007829|PDB:1LR0"
FT STRAND 278..284
FT /evidence="ECO:0007829|PDB:1LR0"
FT STRAND 288..297
FT /evidence="ECO:0007829|PDB:1LR0"
FT HELIX 302..315
FT /evidence="ECO:0007829|PDB:1LR0"
FT HELIX 319..323
FT /evidence="ECO:0007829|PDB:1LR0"
FT HELIX 326..332
FT /evidence="ECO:0007829|PDB:1LR0"
FT STRAND 334..340
FT /evidence="ECO:0007829|PDB:1LR0"
FT HELIX 342..347
FT /evidence="ECO:0007829|PDB:1LR0"
SQ SEQUENCE 347 AA; 37935 MW; EEDD4B04AA095945 CRC64;
MKQQFERSPS ESYFWPVVLA VVLHVLIFAM LFVSWAFAPE LPPSKPIVQA TLYQLKSKSQ
ATTQTNQKIA GEAKKTASKQ YEVEQLEQKK LEQQKLEQQK LEQQQVAAAK AAEQKKADEA
RKAEAQKAAE AKKADEAKKA AEAKAAEQKK QADIAKKRAE DEAKKKAAED AKKKAAEDAK
KKAAEEAKKK AAAEAAKKKA AVEAAKKKAA AAAAAARKAA EDKKARALAE LLSDTTERQQ
ALADEVGSEV TGSLDDLIVN LVSQQWRRPP SARNGMSVEV LIEMLPDGTI TNASVSRSSG
DKPFDSSAVA AVRNVGRIPE MQQLPRATFD SLYRQRRIIF KPEDLSL
