BTHD_DROME
ID BTHD_DROME Reviewed; 249 AA.
AC Q9VYB0; Q8MRZ5; Q962X5;
DT 04-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 5.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Selenoprotein BthD;
DE AltName: Full=dSelM;
DE Flags: Precursor;
GN Name=BthD; ORFNames=CG11177;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=11389138; DOI=10.1074/jbc.m100422200;
RA Martin-Romero F.J., Kryukov G.V., Lobanov A.V., Carlson B.A., Lee B.J.,
RA Gladyshev V.N., Hatfield D.L.;
RT "Selenium metabolism in Drosophila: selenoproteins, selenoprotein mRNA
RT expression, fertility, and mortality.";
RL J. Biol. Chem. 276:29798-29804(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11493597; DOI=10.1093/embo-reports/kve151;
RA Castellano S., Morozova N., Morey M., Berry M.J., Serras F., Corominas M.,
RA Guigo R.;
RT "In silico identification of novel selenoproteins in the Drosophila
RT melanogaster genome.";
RL EMBO Rep. 2:697-702(2001).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=14612395; DOI=10.1128/mcb.23.23.8495-8504.2003;
RA Kwon S.Y., Badenhorst P., Martin-Romero F.J., Carlson B.A., Paterson B.M.,
RA Gladyshev V.N., Lee B.J., Hatfield D.L.;
RT "The Drosophila selenoprotein BthD is required for survival and has a role
RT in salivary gland development.";
RL Mol. Cell. Biol. 23:8495-8504(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-147, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
CC -!- FUNCTION: May be involved in a redox-related process. Required for
CC survival and specifically for salivary gland morphogenesis.
CC {ECO:0000269|PubMed:11493597, ECO:0000269|PubMed:14612395}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14612395}. Secreted
CC {ECO:0000269|PubMed:14612395}. Note=Secreted into the salivary gland
CC lumen.
CC -!- TISSUE SPECIFICITY: Expressed in the developing salivary gland at late
CC stages of embryogenesis. Also expressed in brain, neuroblast and wing
CC disk. {ECO:0000269|PubMed:11493597, ECO:0000269|PubMed:14612395}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically, low
CC level of expression in larvae. Expression in embryos is found within
CC syncytial blastoderm, cellular blastoderm and gastrulation stages.
CC {ECO:0000269|PubMed:11389138, ECO:0000269|PubMed:11493597}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM51045.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF396455; AAK72982.1; -; mRNA.
DR EMBL; AE014298; AAF48293.3; -; Genomic_DNA.
DR EMBL; AY119185; AAM51045.1; ALT_SEQ; mRNA.
DR RefSeq; NP_572903.3; NM_132675.3.
DR BioGRID; 58694; 19.
DR STRING; 7227.FBpp0073637; -.
DR iPTMnet; Q9VYB0; -.
DR PaxDb; Q9VYB0; -.
DR PRIDE; Q9VYB0; -.
DR EnsemblMetazoa; FBtr0073806; FBpp0073637; FBgn0030501.
DR GeneID; 32317; -.
DR KEGG; dme:Dmel_CG11177; -.
DR CTD; 32317; -.
DR FlyBase; FBgn0030501; BthD.
DR VEuPathDB; VectorBase:FBgn0030501; -.
DR eggNOG; ENOG502R8DX; Eukaryota.
DR HOGENOM; CLU_1246551_0_0_1; -.
DR InParanoid; Q9VYB0; -.
DR BioGRID-ORCS; 32317; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32317; -.
DR PRO; PR:Q9VYB0; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030501; Expressed in cleaving embryo and 14 other tissues.
DR ExpressionAtlas; Q9VYB0; baseline and differential.
DR Genevisible; Q9VYB0; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IDA:FlyBase.
DR GO; GO:0045454; P:cell redox homeostasis; TAS:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IDA:UniProtKB.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IDA:UniProtKB.
DR GO; GO:0007436; P:larval salivary gland morphogenesis; IMP:FlyBase.
DR GO; GO:0051775; P:response to redox state; NAS:UniProtKB.
DR GO; GO:0007432; P:salivary gland boundary specification; IDA:UniProtKB.
DR InterPro; IPR011893; Selenoprotein_Rdx-typ.
DR TIGRFAMs; TIGR02174; CXXU_selWTH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Phosphoprotein; Redox-active center;
KW Reference proteome; Secreted; Selenocysteine; Signal.
FT SIGNAL 1..?
FT /evidence="ECO:0000255"
FT CHAIN ?..249
FT /note="Selenoprotein BthD"
FT /id="PRO_0000020835"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 122..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 122..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..249
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_STD 37
FT /note="Selenocysteine"
FT MOD_RES 147
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT CROSSLNK 34..37
FT /note="Cysteinyl-selenocysteine (Cys-Sec); redox-active"
FT /evidence="ECO:0000250"
FT CONFLICT 114
FT /note="Q -> R (in Ref. 2; AAF48293)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="T -> I (in Ref. 2; AAF48293)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="S -> L (in Ref. 2; AAF48293)"
FT /evidence="ECO:0000305"
FT CONFLICT 145
FT /note="I -> L (in Ref. 2; AAF48293)"
FT /evidence="ECO:0000305"
FT CONFLICT 161
FT /note="K -> E (in Ref. 2; AAF48293)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 249 AA; 27774 MW; 76B5483CF5A9C095 CRC64;
MPPKRNKKAE APIAERDAGE ELDPNAPVLY VEHCRSURVF RRRAEELHSA LRERGLQQLQ
LQLNALGAPR RGAFELSLSA GGMGKQEQVA LWSGLKRGPP RARKFPTVEE VYDQIVGILG
DQQESKEQTN TQKSSKIDLP GSEAIASPKK SESTEEAQEN KAPTSTSTSR KSKKEQKSEE
EPTQVDSKEA KQSKELVKTK RQPKAQKKQA KASESQEEVA EDKPPSSQKR KRTTRSSTDE
ATAGAKRRR
