ID   TOLB_PSEAE              Reviewed;         432 AA.
AC   P50601;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   08-DEC-2000, sequence version 2.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=Tol-Pal system protein TolB {ECO:0000255|HAMAP-Rule:MF_00671};
DE   Flags: Precursor;
GN   Name=tolB {ECO:0000255|HAMAP-Rule:MF_00671}; OrderedLocusNames=PA0972;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PAO;
RX   PubMed=10735848; DOI=10.1128/jb.182.8.2077-2087.2000;
RA   Duan K., Lafontaine E.R., Majumdar S., Sokol P.A.;
RT   "RegA, iron, and growth phase regulate expression of the Pseudomonas
RT   aeruginosa tol-oprL gene cluster.";
RL   J. Bacteriol. 182:2077-2087(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
RC   STRAIN=PAO;
RX   PubMed=8955385; DOI=10.1128/jb.178.24.7059-7068.1996;
RA   Dennis J.J., Lafontaine E.R., Sokol P.A.;
RT   "Identification and characterization of the tolQRA genes of Pseudomonas
RT   aeruginosa.";
RL   J. Bacteriol. 178:7059-7068(1996).
CC   -!- FUNCTION: Part of the Tol-Pal system, which plays a role in outer
CC       membrane invagination during cell division and is important for
CC       maintaining outer membrane integrity. {ECO:0000255|HAMAP-
CC       Rule:MF_00671}.
CC   -!- SUBUNIT: The Tol-Pal system is composed of five core proteins: the
CC       inner membrane proteins TolA, TolQ and TolR, the periplasmic protein
CC       TolB and the outer membrane protein Pal. They form a network linking
CC       the inner and outer membranes and the peptidoglycan layer.
CC       {ECO:0000255|HAMAP-Rule:MF_00671}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000255|HAMAP-Rule:MF_00671,
CC       ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TolB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00671, ECO:0000305}.
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DR   EMBL; AE004091; AAG04361.1; -; Genomic_DNA.
DR   EMBL; U39558; AAC44661.2; -; Genomic_DNA.
DR   PIR; F83525; F83525.
DR   RefSeq; NP_249663.1; NC_002516.2.
DR   RefSeq; WP_003112572.1; NZ_QZGE01000007.1.
DR   PDB; 6S3W; NMR; -; B=22-34.
DR   PDBsum; 6S3W; -.
DR   AlphaFoldDB; P50601; -.
DR   BMRB; P50601; -.
DR   SMR; P50601; -.
DR   STRING; 287.DR97_965; -.
DR   PaxDb; P50601; -.
DR   PRIDE; P50601; -.
DR   EnsemblBacteria; AAG04361; AAG04361; PA0972.
DR   GeneID; 881864; -.
DR   KEGG; pae:PA0972; -.
DR   PATRIC; fig|208964.12.peg.1010; -.
DR   PseudoCAP; PA0972; -.
DR   HOGENOM; CLU_047123_0_0_6; -.
DR   InParanoid; P50601; -.
DR   OMA; VREPSWG; -.
DR   PhylomeDB; P50601; -.
DR   BioCyc; PAER208964:G1FZ6-993-MON; -.
DR   PHI-base; PHI:3137; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR   GO; GO:0017038; P:protein import; IEA:InterPro.
DR   Gene3D; 2.120.10.30; -; 1.
DR   HAMAP; MF_00671; TolB; 1.
DR   InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR   InterPro; IPR011659; PD40.
DR   InterPro; IPR014167; Tol-Pal_TolB.
DR   InterPro; IPR007195; TolB_N.
DR   Pfam; PF07676; PD40; 4.
DR   Pfam; PF04052; TolB_N; 1.
DR   TIGRFAMs; TIGR02800; propeller_TolB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell cycle; Cell division; Periplasm; Reference proteome;
KW   Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00671"
FT   CHAIN           22..432
FT                   /note="Tol-Pal system protein TolB"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00671"
FT                   /id="PRO_0000034672"
FT   STRAND          25..27
FT                   /evidence="ECO:0007829|PDB:6S3W"
SQ   SEQUENCE   432 AA;  47752 MW;  2D2773B82B85B4BC CRC64;
     MSTLIRIALF ALALMAGAAQ AADPLVISSG NDRAIPIAVV PFGFQGGNVL PEDMSNIIGN
     DLRNSGYFEP LPRQNMISQP AQASEVIFRD WKAVGVNYVM VGNIVPAGGR LQVQYALFDV
     GTEQQVLTGS VTGSTDQLRD MSHYIADQSF EKLTGIKGAF STKMLYVTAE RFSVDNTRYT
     LQRSDYDGAR PVTLLQSREP IVSPRFSPDG RRIAYVSFEQ KRPRIFIQYV DTGRREQITN
     FEGLNGAPAF SPDGNRLAFV LSRDGNPEIY VMDLGSRALR RLTNNLAIDT EPFWGKDGST
     LYFTSDRGGK PQIYKMNVNS GAVDRVTFIG NYNANPKLSA DEKTLVMVHR QQGYTNFQIA
     AQDLQRGNLR VLSNTTLDDS PTVAPNGTML IYATRQQDRG VLMLVSINGR VRIPLPTAQG
     DVREPSWSPY LN