BTN1_SCHPO
ID BTN1_SCHPO Reviewed; 396 AA.
AC Q9US09;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Protein btn1;
GN Name=btn1 {ECO:0000312|EMBL:CAB63796.1}; ORFNames=SPAC607.09c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-137; GLU-240 AND
RP VAL-278.
RX PubMed=16291725; DOI=10.1242/jcs.02656;
RA Gachet Y., Codlin S., Hyams J.S., Mole S.E.;
RT "btn1, the Schizosaccharomyces pombe homologue of the human Batten disease
RT gene CLN3, regulates vacuole homeostasis.";
RL J. Cell Sci. 118:5525-5536(2005).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Involved in vacuolar transport and vacuole pH homeostasis.
CC Also required for cytokinesis. {ECO:0000269|PubMed:16291725}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC membrane protein. Vacuole membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the battenin family. {ECO:0000255}.
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DR EMBL; CU329670; CAB63796.1; -; Genomic_DNA.
DR PIR; T50229; T50229.
DR RefSeq; NP_593598.1; NM_001019029.2.
DR AlphaFoldDB; Q9US09; -.
DR SMR; Q9US09; -.
DR BioGRID; 280059; 469.
DR STRING; 4896.SPAC607.09c.1; -.
DR SwissPalm; Q9US09; -.
DR PaxDb; Q9US09; -.
DR EnsemblFungi; SPAC607.09c.1; SPAC607.09c.1:pep; SPAC607.09c.
DR GeneID; 2543645; -.
DR KEGG; spo:SPAC607.09c; -.
DR PomBase; SPAC607.09c; btn1.
DR VEuPathDB; FungiDB:SPAC607.09c; -.
DR eggNOG; KOG3880; Eukaryota.
DR HOGENOM; CLU_029663_1_2_1; -.
DR InParanoid; Q9US09; -.
DR OMA; WLCNWQV; -.
DR PhylomeDB; Q9US09; -.
DR PRO; PR:Q9US09; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0032153; C:cell division site; IDA:PomBase.
DR GO; GO:0051286; C:cell tip; IDA:PomBase.
DR GO; GO:0005737; C:cytoplasm; IDA:PomBase.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:PomBase.
DR GO; GO:0030176; C:integral component of endoplasmic reticulum membrane; IC:PomBase.
DR GO; GO:0005773; C:vacuole; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; ISM:PomBase.
DR GO; GO:1903826; P:L-arginine transmembrane transport; ISO:PomBase.
DR GO; GO:0051453; P:regulation of intracellular pH; IBA:GO_Central.
DR InterPro; IPR003492; Battenin_disease_Cln3.
DR InterPro; IPR018460; Battenin_disease_Cln3_subgr.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR10981; PTHR10981; 1.
DR Pfam; PF02487; CLN3; 1.
DR PIRSF; PIRSF015974; CLN3_BTN1; 1.
DR PRINTS; PR01315; BATTENIN.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Endoplasmic reticulum; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..396
FT /note="Protein btn1"
FT /id="PRO_0000065006"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 234..254
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 296..316
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 137
FT /note="G->A: Retarded location in the endoplasmic
FT reticulum; enlarged vacuoles."
FT /evidence="ECO:0000269|PubMed:16291725"
FT MUTAGEN 240
FT /note="E->K: Normal trafficking to vacuolar membrane but
FT slower than wild-type; enlarged vacuoles."
FT /evidence="ECO:0000269|PubMed:16291725"
FT MUTAGEN 278
FT /note="V->F: Normal trafficking to vacuolar membrane but
FT slower than wild-type; enlarged vacuoles."
FT /evidence="ECO:0000269|PubMed:16291725"
SQ SEQUENCE 396 AA; 44014 MW; 3550AA3E46936C2D CRC64;
MIKLRLTKDA KVGCCFLIFG LLNNLLYVII LSAALDLVGA NVSKGVVLLS NIVPSLACKL
SASILHVHKF KFAKRIGFCV FMSILGMQWI AWSSSVPSKM LGVSLAAISS SFGEISFLHL
SSRYHSVSLP CWSSGTGLAG LFGASSYLVM TTWFNFSVRS TLIISSFLPL FLLIMYFFVL
PESESTSPSI NNNYTPIESI DLRAGHVSFN FVNSLKQTFI FMQPYLLSHM FPQFLVYFSE
YTINIGVAPT LLFPPEKAGF SSFRDFYPTY QTVYQIGVFL SRSSISFFTV PYLRTLAITQ
FIILLFTILQ SALYLTSSYH FVLFLIFVEG LIGGTVYVNV YHSLQTTESS QRELAISTVG
SSDSSGIFLA SLVSLFLEPS LCHFQADRGR DWCALT
