ID   TOLC_SALEN              Reviewed;         491 AA.
AC   Q54001;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Outer membrane protein TolC;
DE   AltName: Full=Multidrug efflux pump subunit TolC;
DE   Flags: Precursor;
GN   Name=tolC; Synonyms=sinA;
OS   Salmonella enteritidis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=149539;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   STRAIN=CDC5;
RX   PubMed=8801424; DOI=10.1111/j.1365-2958.1995.mmi_17040701.x;
RA   Stone B.J., Miller V.L.;
RT   "Salmonella enteritidis has a homologue of tolC that is required for
RT   virulence in BALB/c mice.";
RL   Mol. Microbiol. 17:701-712(1995).
CC   -!- FUNCTION: Outer membrane channel, which is required for the function of
CC       several efflux systems (By similarity). Required for virulence.
CC       {ECO:0000250, ECO:0000269|PubMed:8801424}.
CC   -!- SUBUNIT: Homotrimer. Part of tripartite efflux systems, which are
CC       composed of an inner membrane transporter, a periplasmic membrane
CC       fusion protein, and an outer membrane component, TolC. The complexes
CC       form a large protein conduit and can translocate molecules across both
CC       the inner and outer membranes (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- INDUCTION: Constitutively expressed (at protein level).
CC       {ECO:0000269|PubMed:8801424}.
CC   -!- DISRUPTION PHENOTYPE: Non-invasive for a number of mammalian cell
CC       lines; avirulent by oral infection of BALB/c mice. Increased
CC       sensitivity to acridine orange and novibiocin. Decreased resistance to
CC       bile salts and SDS. {ECO:0000269|PubMed:8801424}.
CC   -!- SIMILARITY: Belongs to the outer membrane factor (OMF) (TC 1.B.17)
CC       family. {ECO:0000305}.
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DR   EMBL; U25178; AAC43973.1; -; Genomic_DNA.
DR   PIR; S70190; S70190.
DR   AlphaFoldDB; Q54001; -.
DR   SMR; Q54001; -.
DR   PRIDE; Q54001; -.
DR   PHI-base; PHI:6553; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR003423; OMP_efflux.
DR   InterPro; IPR010130; T1SS_OMP_TolC.
DR   Pfam; PF02321; OEP; 2.
DR   TIGRFAMs; TIGR01844; type_I_sec_TolC; 1.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell outer membrane; Membrane; Signal;
KW   Transmembrane; Transmembrane beta strand; Transport; Virulence.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000250"
FT   CHAIN           25..491
FT                   /note="Outer membrane protein TolC"
FT                   /id="PRO_0000013353"
FT   REGION          267..287
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          445..491
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        476..491
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   491 AA;  53726 MW;  58F0FB64C0257FA4 CRC64;
     MQMKKLLPIL IGLSLSGFST LSQAENLMQV YQQARLSNPE LRKSAADRDA AFEKINEARS
     PLLPQLGLGA DYTYSNGYRD ANGINSNETS ASLQLTQTLF DMSKWRGLTL QEKAAGIQDV
     TYQTDQQTLI LNTANAYFKV LNAIDVLSYT QAQKEAIYRQ LDQTTQRFNV GLVAITDVQN
     ARAQYDTVLA NEVTVRNNLD NAVEELRQVT GNYYPELASL NVEHFKTDKP KAVNALLKEA
     ENRNLSLLQA RLSQDLAREQ IRQAQDGHLP TLNLTPSTGI SDTSYSGSKT NAAQYDDSNM
     GQNKIGLNFS LPLYQGGMVN SQVKQAQYNF VGASEQLESA HRSVVQTVRS SFNNINASIS
     SINAYKQAVV SAQSSLDAME AGYSVGTRTI VDVLDATTTL YDAKQQLANA RYTYLINQLN
     IKYALGTLNE QDLLALNSTL GKPIPTSPES VAPETPDQDC AADGYNAHSA APAVQPTAAR
     ANSNNGNPFR H