ID   TOM1A_MISBR             Reviewed;          39 AA.
AC   P83588;
DT   27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 52.
DE   RecName: Full=Omega-actinopoditoxin-Mb1a;
DE            Short=Omega-AOTX-Mb1a;
DE   AltName: Full=Omega-missulenatoxin-Mb1a;
DE            Short=Omega-MSTX-Mb1a;
OS   Missulena bradleyi (Eastern mouse spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Actinopodidae; Missulena.
OX   NCBI_TaxID=230234 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom {ECO:0000305};
RA   Chong Y., Khalife A.A., Hains P.G., Broady K.R., Nicholson G.M.;
RL   Submitted (MAY-2003) to UniProtKB.
CC   -!- FUNCTION: Potent inhibitor of insect, but not mammalian, voltage-gated
CC       calcium channels (Cav). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1, ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000269|Ref.1,
CC       ECO:0000305}.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- PTM: Contains 3 disulfide bonds. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=4167; Method=Electrospray;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- SIMILARITY: Belongs to the neurotoxin 08 (Shiva) family. 02
CC       (omega/kappa toxin) subfamily.
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DR   AlphaFoldDB; P83588; -.
DR   SMR; P83588; -.
DR   ArachnoServer; AS000184; omega-actinopoditoxin-Mb1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019855; F:calcium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   InterPro; IPR009415; Omega-atracotox.
DR   InterPro; IPR018071; Omega-atracotox_CS.
DR   Pfam; PF06357; Omega-toxin; 1.
DR   PROSITE; PS60016; OMEGA_ACTX_1; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated calcium channel impairing toxin.
FT   PEPTIDE         1..39
FT                   /note="Omega-actinopoditoxin-Mb1a"
FT                   /id="PRO_0000044995"
FT   SITE            10
FT                   /note="Critical for insecticidal activity"
FT                   /evidence="ECO:0000250"
FT   SITE            29
FT                   /note="Critical for insecticidal activity"
FT                   /evidence="ECO:0000250"
FT   SITE            37
FT                   /note="Critical for insecticidal activity"
FT                   /evidence="ECO:0000250"
FT   DISULFID        4..19
FT                   /evidence="ECO:0000250"
FT   DISULFID        11..30
FT                   /evidence="ECO:0000250"
FT   DISULFID        18..38
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   39 AA;  4173 MW;  871520FC066A41B6 CRC64;
     SPVCTPSGQP CQPNTQPCCN NAEEEQTINC NGNTVYRCA