TOM1_ASHGO
ID TOM1_ASHGO Reviewed; 3258 AA.
AC Q756G2;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Probable E3 ubiquitin-protein ligase TOM1;
DE EC=2.3.2.26;
DE AltName: Full=HECT-type E3 ubiquitin transferase TOM1;
GN Name=TOM1; OrderedLocusNames=AER304C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 2812.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Probable ubiquitin ligase protein, which may be involved in
CC mRNA export. E3 ubiquitin ligase proteins mediate ubiquitination and
CC subsequent proteasomal degradation of target proteins. Participates in
CC mRNA export from the nucleus by regulating the transport of hnRNP
CC proteins. {ECO:0000250|UniProtKB:Q03280}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q03280}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000305}.
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DR EMBL; AE016818; AAS52985.2; -; Genomic_DNA.
DR RefSeq; NP_985161.2; NM_210515.2.
DR SMR; Q756G2; -.
DR STRING; 33169.AAS52985; -.
DR PRIDE; Q756G2; -.
DR EnsemblFungi; AAS52985; AAS52985; AGOS_AER304C.
DR GeneID; 4621374; -.
DR KEGG; ago:AGOS_AER304C; -.
DR eggNOG; KOG0939; Eukaryota.
DR HOGENOM; CLU_000215_0_1_1; -.
DR InParanoid; Q756G2; -.
DR OMA; HGLLFQI; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000591; Chromosome V.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF14377; UBM; 2.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR SUPFAM; SSF56204; SSF56204; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW mRNA transport; Nucleus; Reference proteome; Transferase; Transport;
KW Ubl conjugation pathway.
FT CHAIN 1..3258
FT /note="Probable E3 ubiquitin-protein ligase TOM1"
FT /id="PRO_0000120345"
FT DOMAIN 2922..3258
FT /note="HECT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
FT REGION 1582..1603
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1945..1981
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2036..2074
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2254..2298
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2411..2436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1582..1596
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1956..1981
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2254..2285
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3225
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3258 AA; 372401 MW; 0751CA476D9646A2 CRC64;
MVVTLTKLER LQKAEKSRTF KPLIDELINC EDAVFVGKLE AIREWDRPKD DLFLWIPVLD
RMDDMLGKVV AKYKYHTTDC KRHPVKLIEM AKPDEDWVAT LLFFTCRLLN NTSNRSLYSS
LDMMSDLLNC PNFRVKLGAM KVIATIGERH VVARHRIENS SVLASQHLKK RSLSLALALP
SSTTDDNSDH FSLVDLFFDK RKYPSKWSQL QYTYYITKKQ AGQQSTQKPS QVSSMKRFVL
NNEELRFLSL QQIFDRAMND LPSEFWFDFS LQASIAKAFS DDSFENIQLR SLIIQTKFAA
IAFANAIYIP PQVSSKLFEM DPYAFNNLTD FISLSETKIP KDLRTDALFA LECISLKHVW
CSDIVRNLGG NMSHGLLFQI LRYIAKVLRE GDEDAVDEEY NVRFFYLISN LADVKALQES
LISAGLISSL LEIVSTQNSK YKRTLASAAH LLEDVISDAD ATAEFINNNG FNILIQTVTY
EVNFAVQNPS SAEPPKYSVV YYSISFRQLG FIRSLLKLVL KLLKTDSGDR IRNLIDSPIL
LAFNKILENR PVFGYTLVSH ALDVVQTIIN TEPTIYQVLV ESGTIPYILQ NFDQFLGPTS
DLLCMLPEVI SAICLNTDGL KQVKEKNLLK YLFQVIKTPE FAKILSWEDQ AVNYGVALDE
LARHYPELKP LIEDYFAATV KELPSLTSYT HSYLYESTAG NGEFYLSENE TIIDNEDGAD
ELAFWEVQES SPIIDCFSGV FYSMASENIS WANLTEKIAF QDFLSVAIPE KPTFDYINSQ
TLLNFTDVLK MFDDERRSYA LPELLTILDS KFADLEDFLS YDFTKSYVLN EPKDRVVATL
NKLNVLNVIL YIMTDIYINI TTLFPVRVIQ MMEFFEKNGF NLITNLRKMF QRCVLEEMYI
RSRLPPAVAE ETISPGISFV PPILIHKDVP LKTEAKQEKT SAKFKNTLET RHLFQKVQSW
TSMLFRCFLR LTHARKMNVE RYDRALEIRI FDKVVNEIVE MLNLDYLDSH LSYFLVVLDF
NTHIFTCPKA SLTISDGIVQ TVPTFLFYQA GGFAIYHELI KKLSTRLLGF DGIEAIEKVE
YVKDQDDILT VGVLMNSIAF MNRCIQLETM ENIRSIAEYY PYDDIYYNLT RALIVPVKIL
SLGAISEIFS QGEVFDTDRR RIPYSVFKQI LSLLKNIYNS TFELEEGTDV YELRWDLMPI
SHRKVEMLKS CGISHDVAVG YLEEEKDELP IHVKPDVFSD SEWQRYQEER SSGSWRTNIE
LLPPQYNGSS TRETLSKMRT EFYQNGFESG ILKILQHYPK LINAISHMFF EMYGELGFSH
TTMLEDLQDM MNTIPIENTN KLAPVIHLFG IFLNDKNIYD QAKKEIFKFV SYLSMNLHPE
NVNYSWFSKA LYVYEIIFAK SETPEASPLP ENVTVSFSSI PIIYRISQKD KDIIFNLLIR
ANEVTDFYSA LAISRILILY TREERYAQEV TQSGILSKLL KVIGANQKFD KINYLESSYL
LLVRRCFETK EIVTSLIDYE LTRAFTTRAI GDHKEKPRDL PALVNEKASI VMRDPEVFVN
RISETARFED FNSSKELASL SVRRHMDEKD EEMTSKPLSS EGSNSPITGI VHLLLSQLMA
AHKRDWVSET PLTPEEENQK NKKKKDEVKV SKNPVCAYMI FLLKVLTELV SSYKQSKFEF
LTYNKKNVYN ETPKPRATAL NFFLYQLLDT NFQDMDKHEG KRRAMISGLA RDTIIGFVSS
VQDSETKKVD PKIVDSDMTY IRKFTIEAIS KAIKEAGVSA KTIDANAGKL FGWFHLISSL
LVVDKGYIFS VLDSNKSSND KYQLCKLLIE MNTPGTITDC MASLDLNYPL TKKLFNSAVE
PLNALNEVRN NFADLFKLEN NEDEDVEDVE SDKDDVPDMF KNSALGMYDI EDVEDDHDDD
ENESLIGDDE DIAFVHDEDG LEVVFSEDED ANEDTTDASN SIGSDSEGNS DFGGSGPNVT
LEVYTDSEDA IEDVNTAAIR ITSGNSAEHS YYSEGEDSAE IEIYEEEYDS EIDIDMDDDS
ELGSSNWESG LSDLSDSEAY SDEERTNNTG DGFVRWYSDD GVEFEDDTDE EGRGLFTGIQ
HVFPTEEQLF RVHASGAARS TGRHHHRHGA APFTTSTITL GASQRRPHSI LSNPLGPSGL
EEVENDIVSH YLGNVETSDR IGLSSIPRLP RVLLFDGELF DDKSSSGILL KSTTARWNDI
YEMFYDSKVY SNNVVTTIIS RIFEPSAELY LKEQEENAVK ESSRINEPTR RQDERKRKLH
EIDSDEEHIE EEEEHDEVVE PIEAPGINSP QARAPQEPVY VTIDGEAVNI AGTDMDAEFL
NALPDDIRAE VFAQHIREYR TQFQGSEGSS RELDAGFLNT IPETLRQEIL AQEVPLERNA
RPSILGLRNR EGEEFSEVED ESPRFNEQRT ESSKTKTDRV HFAPLLDRSG IAAIMKAVFI
PQPYLSREIY HELFYRLCSS KQNRSDIMNM LLLILMDGIN DQHSLERVYN LLSNRAASSN
SGTSKTPQRQ LPPDCTPLIV TNQCIEILQS MVDADSKLKY FFITEHENLL INKSPLKNKK
DIFSKNMKWP INCILALLEK KVITDEAVLM DLLTRILQVC SKPISSIVKS SKDGKKKKFE
VPDIEKKYLA SIVSIIKLDS CNTKVFQQTL NLMTNLFAIK DAHETFTTEL CNLAKETIEV
LVTDLDALAK EVPAVDSGTE VSSEIIQKFT VPSSDQSKLL KVLTAIDYIY VNRKKEEEQV
VDQLLPLYNK MELGHIWVSL SNCLTRFEEK PRMSTSATIL LPLIESLMVV CKHSKVRETK
DALLKYEAKK CDFARTPVEN LFFAFTDLHK KLLNEMIRSN PKLMSGPFSL LVKNPKILDF
DNKRYYFTAQ LRAITHDRPK LSISVRREHV FLDSYRSLFF KSNEDIKISK LEISFKGEAG
VDAGGITREW YQVLSRQMFN PDYALFIPVA SDKTTFRPNR TSGINPEHLS FFKFIGMIIG
KAISDQCFLD CHFSREVYKN ILGKPVALKD MESLDLDYYK SLIWILENDI TDIIEETFSV
ETDDYGEHKV IELIENGAHV AVTEQNKHDY VKKIVEYKLQ TSVKDQMENF LQGFYAIIPK
DLISIFDEQE LELLVSGLPD IDVDDWKNNT IYVNYTPTCK QINYFWRAVR SFDKEERAKL
LQFVTGTSKV PLNGFKELSG VNGISKFSIH RDYGSIDRLP SSHTCFNQLD LPAYDSYETL
RGSLLLAINE GHEGFGIA
