TOM1_NEUCR
ID TOM1_NEUCR Reviewed; 4076 AA.
AC Q9P4Z1;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 4.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=E3 ubiquitin-protein ligase TOM1-like;
DE EC=2.3.2.26;
DE AltName: Full=HECT-type E3 ubiquitin transferase TOM1-like;
GN ORFNames=B11B22.010, NCU08501;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Probable ubiquitin ligase protein, which may be involved in
CC mRNA export. E3 ubiquitin ligase proteins mediate ubiquitination and
CC subsequent proteasomal degradation of target proteins. Participates in
CC mRNA export from the nucleus by regulating the transport of hnRNP
CC proteins. {ECO:0000250|UniProtKB:Q03280}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB92704.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL356834; CAB92704.2; ALT_SEQ; Genomic_DNA.
DR EMBL; CM002237; EAA34194.3; -; Genomic_DNA.
DR PIR; T49799; T49799.
DR RefSeq; XP_963430.3; XM_958337.3.
DR SMR; Q9P4Z1; -.
DR STRING; 5141.EFNCRP00000008460; -.
DR PRIDE; Q9P4Z1; -.
DR EnsemblFungi; EAA34194; EAA34194; NCU08501.
DR GeneID; 3879570; -.
DR KEGG; ncr:NCU08501; -.
DR VEuPathDB; FungiDB:NCU08501; -.
DR HOGENOM; CLU_000215_0_1_1; -.
DR InParanoid; Q9P4Z1; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000001805; Chromosome 6, Linkage Group II.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0016567; P:protein ubiquitination; IBA:GO_Central.
DR CDD; cd00078; HECTc; 1.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; SSF56204; 1.
DR PROSITE; PS50237; HECT; 1.
PE 3: Inferred from homology;
KW Coiled coil; mRNA transport; Nucleus; Reference proteome; Transferase;
KW Transport; Ubl conjugation pathway.
FT CHAIN 1..4076
FT /note="E3 ubiquitin-protein ligase TOM1-like"
FT /id="PRO_0000120346"
FT DOMAIN 3740..4076
FT /note="HECT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
FT REGION 225..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 288..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 748..819
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 921..970
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1083..1103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1571..1646
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1988..2041
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2067..2110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2275..2295
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2356..2551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2581..2634
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2782..2817
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2858..2955
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3037..3066
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3105..3132
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3216..3241
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3353..3444
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 2851..2929
FT /evidence="ECO:0000255"
FT COILED 3341..3375
FT /evidence="ECO:0000255"
FT COMPBIAS 237..251
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 748..765
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 939..954
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 955..970
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1590..1643
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1998..2017
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2018..2041
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2082..2096
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2403..2451
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2468..2551
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2581..2596
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2597..2624
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2858..2912
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3353..3372
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3375..3416
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3417..3438
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4043
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4076 AA; 453670 MW; 0ED7E2C532706D5D CRC64;
MGKITKTMQQ KHRDTLSPWL KEFVDTASSA PLPLILQRLD EFPRRWMFPR GDLYHWIPLL
NRFDNILESI CATYELSKGP QTRDFGRDVL LNNGGPSLEY RDEPWTVERL AEAGYKEDGD
CQLLVAILKF TRMLLEHCGN RSIYASSHHI DRLLNSPYLE VQAAALEVGL ELAQRYNASV
KRMPSPPRSV NPTLLANHYN IDLEKVQLLA RPFVRTPIVK SLEASSAAPA VSAGSTAKAK
DKEKEKEKAT GPKNVASMYA NDLKALASSR PDEEDLWKSW GDLKMSYYPD TTNGEPSARD
SKDAQPVEPR TTSSPAAPTP LRRSSTMNVS QSSRTQRVGS SEEGSPLKPS GAATDDRDGP
KVVEIPRSVI ESKRIYDLYD MCPSDMPATM KFEFTSRLRT CKALLGTHRE RQLALAVRLL
AITNLAYILP EAVFVDKVLK YDKDEPRTFQ LVYQLAELIA PSPDGNPSEV PKWLQSITLA
LLEAISHQQE KHSDVLAALN VNVNHGILLY VIRIAVAEMK EDAPVDDQDE LDADNWRGNL
FGLTLQIAMA TRIGQEMMTA GLMDCLVEIL NLRTAVASRH YSMVLAFLDS LTYAYQTAFS
QLNSAGGLDA ITNLIVHTVG ESKTLTEAGL GTKPELHASL VDYSIPFYHQ QTLKWLLKFI
HHVMANTYSF DGTNTERLLR NLVDNSSLLG SLCTIARQNR FFGTVVWSNA TTLLSDFINN
DPTSFAAISE SGWIQAFLES VTNRPVSIPA EQPSLEPSQS RANDGSEGND ADDDSEDDGS
AHDAAPDSDG QAATQPHPPT QEMLEAPRDF PPAHGILPSS ESMNIIPTVL NSISLNNRGM
KMVLSSGAIQ SYMEIFESAT HVQCMAHDPE LASTIGSSLD ELSRHHPALR PAIANAIIDM
IARVTHLVKT MDATKACGAR LEAPESSASP VAEPAQATEV KGKGKEKATD DTDVEMAEAS
SSSSGNNKPA QAPSIPYIQV LSTFLQPIIS NSHLKGALIS AGVIEILLDL AESPSLPHDF
GETPACRMLV AAISQLIESA QIVGLPSLLF RMENTVKVLQ PRINPTTTEP FFAPFLTLNS
SVSPVQDEEP ASEKRTPDVS SGTETVKALL NIQTMIKILY HCFPFSNRSQ MVSMPAVNVY
DYYIRLIQSL GPLLRGVLKE EAAVNGAVPH HWTLKNKPYQ TNSLGSRTDV QDLLTDSAAQ
DSSANGKKLT PEEQSTAEYK NFQTLRVLLH SFMPSIFPFF QTIGKALLPR RNNNDPYIRS
RHLAIAEASA ETLIQQLQQS KAELTVRDIH NWIIMIHTFG EMVVDTNHRT ASGGAFLILP
VITAFKELGG FEALNVMLKR LADMVSTGAT EGQEATKAKL SMIGMKKVLD IYCFIISGKN
LSDSMAQIAL APKPTERTTR EFSHQLVVEL RMSILPVVRE IWASNIIEKS TGTVVSKIID
IIKTIAAGDL ESNAQSRSDK ESLPHLFKNR ESVPFKWISR KDAETLATEQ GVDVDLALEA
LYRANGKESD TKEYIKYQKA HLVRNRNPVP AEDAFKEVPS PNLSSSAGMS LSNLLNTPTF
PVSDLLGAEP MALDPVPNQP LGEASGETAL GHATESSEDG SDEGQPGTSR ETNVGASTTA
PQQLPVLPSQ QPATESQSNT PRITREDLVE ERAKLYDTLI DRSLEVISSH PEAVFEVADL
IQNTILKTDN EDRRVEVGEI LANALMSFAS DDADELKENG SSIAAYAHLL ALLLQQTAFM
RTTVDMLRDY VGDYLGFLKL PPASSNDSLP PWIPYILLIF EIMLFKDAQP PDIKWKQPVK
EDDPIEESVI EVKEPNFLAE HRSSLLTTLL DFLPRIGKEE SLAVSVLRIL VVLTRDHAVA
KIVGEKKNLQ RLFVMAKQLC GAGSTRLKQT HISEHIRQIL RHIIEDEETL RQIFETDIRH
LMTSRQRPSA APGLEPQAYL RTQAHLALRS PKTFVEVSTE LLKLNRAVSH LGDGTLRSTP
FLVLKERPAD ASVSPKESSV EPAVQATEDL TISDVKPSTE VTDKDMHDAP KNPAQDLKRP
ILEHPDGVVH FLLTELLNYK DVDERENVPA PPAAASKPES DATAANEATP SPSGDEQNSE
SKEKEKKLAK SPFKAEDHPI FIYRCFLLDC LAELLQSYNR AKVEFINFKR SAPVQANAPV
KPRSSVLNYI LNDLLCFSPA SGVPESIAMK KKAATAVPAR AVLVALVSKT GEKPQNRTHE
PFEYDDEPDL LFIRRFVLDT ILRAYKDASV SGEPADIRYG KMNALAELMA QMIGEGDKDS
RPNNPRGTDP SIGRSQAQLK RLMYEKGYLT SLTASIADID LTFPHNRAPL KPILAVIKTL
SKTAVSMSQL GLIPASGTAG TDQAEDEFLS DGSSVSEDLT DDREETPDLY RNSTLGMLEP
GRDDEFSDED EDDDEDMYDD EQYDDELDYG DDMSQDNEDN PSDEEDDLGE MGEMGGMPGQ
PGVVEVLMGE NDDEEDNDDM DDMDEDDEMD EDDEQELSDE DEDEEVGSED MDDLEDDIHI
VDEEGNAIDD DGASWDDGTD EDEEDEEELD YEAEAQNMQE AQLHNRRTFP EIMRAAMENA
GDDLDAEPIR DFDGHYIDDD EDGEEDDDED EGEDDMDDDM YFDGDGLHDD LLAPNMPSGL
GWDIAIEPNH RHRPSRSPWP NSPFVVGRHR DAIDFQNFFR RPAHLSRHLP PPADVMSGTN
PLLLPQSRRE VPRHAHSQLV RLGITPNMIG GLGMGMGVEP LAFISDLVQH LPDVRLSAGG
GPLALHFTAD GPGGIIRELN AIPIPPPHSR ESRPTEARRD TYQEPHQAVQ FSPESTHERW
QQEVKMIFGF GYQDKAQKLA PLILSKLTPA AIQAEKEEKA RKAEADRKAE EERKKRQEEE
RKKREAKEAE EKAAREKKEA EERERLERER AEAAAQAAAQ AAADQEANAV SQEAHPMEGV
ETQGPGENAE QQAEDERPRV YYTLRNQQID ITELGIDAEY LEALPEEFRD EVIAQAISTR
RSQAREQVSQ EGENTEVFQE FLEALPEELR NEILHQEQHE QRRRERQNAA GGQDLGPADM
DPASILLTFP PGLRQQVLLD QGEDIMEHLG PELAAEARTL VARHRQLHAQ QGGQAASRSR
DAQRPTEAGA GTVQKIQKRT VVQMLDKQGI ATLLRLMFVS QQGSIRSSLF SIFANLCENR
QNRLDVISSL LQILQDGCAN MDAVERSFAQ ISHKAKQLKE KDAKTPHPLK RSLTGGTNNN
GQFPASSEVS PLLIVQQCLD LLVELSKLNP HIPSVFLTEH ETVASTLKRS LSRKGKGKDV
NGKAQKFAIN SLLTLLDRSL VMESSAVMQV LADLLNKVTI PLQAIERRRK EAEEQAKKKK
EAEEKAATER EAANAPEEQA STSTEQTPAQ QEATQQPSES TPAAASGQQP AQQDQENKEL
EAPKEKADEK DVQSDEKKIR QLTPPTIPEH NLKLVINIFV ARECSSKTFQ NTISTIKNLS
NIPGAKKVFG DELVRQARVL SENILSDLDN LLPHILKAES GTQIQGVALA KFSPGASEQN
KLLRVLTALD HLFDSKSKKQ DKPAEGENTK EDLLGSLYWN PTFGKMWDKL SACLSAIRQR
DNMLNVATIL LPLIESLMVV CKNTTLSDAS AVSNANSQKE MLLTSPPPED RIAGLFFTFT
EEHRRILNEL VRHNPKLMSG TFSLLVKNPK VLEFDNKRNY FNRSVHSKYQ QTRHSFPPLQ
LQVRREHVFH DSFRSLYYKK ADELKFGKLN IRFQGEEGVD AGGVTREWFQ VLSRQMFDPN
YVLFVPVSSD RTTFHPNKLS PINDEHLPFF KFIGRIIGKA LYEGRLLECY FSRAVYKRIL
GKPVSVKDME SFDPDYYKSL VWMLENDITD IITETFSVED DVFGEVKVVD LIENGRNIPV
TEENKHEYVR LIVEHKLITS VKDQMKAFLT GFHEIIPEEL IAIFNEQELE LLISGLPDID
IDDWKANTEY HNYSAGAPQI QWFWRAVRSF DKEELAKLLQ FVTGTSKVPL NGFKELEGMN
GVSRFNIHRD YGSKDRLPSS HTCFNQLDLP EYENYETLRS QLLKAITAGS DYFGFA
