ID   TOM20_SOLTU             Reviewed;         204 AA.
AC   P92792;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Mitochondrial import receptor subunit TOM20;
DE   AltName: Full=Translocase of outer membrane 20 kDa subunit;
GN   Name=TOM20;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 19-47 AND 55-63,
RP   CHARACTERIZATION, AND SUBCELLULAR LOCATION.
RC   STRAIN=cv. Desiree; TISSUE=Leaf;
RX   PubMed=8696363; DOI=10.1046/j.1365-313x.1996.9060829.x;
RA   Heins L., Schmitz U.K.;
RT   "A receptor for protein import into potato mitochondria.";
RL   Plant J. 9:829-839(1996).
RN   [2]
RP   PROTEIN SEQUENCE OF 55-63.
RC   TISSUE=Tuber;
RX   PubMed=9642296; DOI=10.1074/jbc.273.27.17251;
RA   Jaensch L., Kruft V., Schmitz U.K., Braun H.-P.;
RT   "Unique composition of the preprotein translocase of the outer
RT   mitochondrial membrane from plants.";
RL   J. Biol. Chem. 273:17251-17257(1998).
CC   -!- FUNCTION: Central component of the receptor complex responsible for the
CC       recognition and translocation of cytosolically synthesized
CC       mitochondrial preproteins. Together with TOM22 functions as the transit
CC       peptide receptor at the surface of the mitochondrion outer membrane and
CC       facilitates the movement of preproteins into the translocation pore.
CC   -!- SUBUNIT: Forms part of the preprotein translocase complex of the outer
CC       mitochondrial membrane (TOM complex).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC       {ECO:0000269|PubMed:8696363}; Single-pass membrane protein
CC       {ECO:0000269|PubMed:8696363}.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- MISCELLANEOUS: In mammals and fungi, the transmembrane domain is
CC       located at the N-terminus while it is located at the C-terminus in
CC       plants. The overall orientation of the protein in the membrane is
CC       therefore inverted.
CC   -!- SIMILARITY: Belongs to the Tom20 family. {ECO:0000305}.
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DR   EMBL; X92491; CAA63223.1; -; mRNA.
DR   PIR; T07679; T07679.
DR   RefSeq; NP_001275135.1; NM_001288206.1.
DR   AlphaFoldDB; P92792; -.
DR   SMR; P92792; -.
DR   GeneID; 102603648; -.
DR   KEGG; sot:102603648; -.
DR   InParanoid; P92792; -.
DR   OrthoDB; 1560087at2759; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P92792; baseline.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IEA:InterPro.
DR   GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IEA:InterPro.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR010547; TOM20_imprt_rcpt.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   PANTHER; PTHR32409; PTHR32409; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Membrane; Mitochondrion;
KW   Mitochondrion outer membrane; Protein transport; Reference proteome;
KW   TPR repeat; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..204
FT                   /note="Mitochondrial import receptor subunit TOM20"
FT                   /id="PRO_0000051547"
FT   TOPO_DOM        1..169
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        191..204
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   REPEAT          86..119
FT                   /note="TPR"
FT   REGION          144..164
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        148..163
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   204 AA;  22814 MW;  F61B5AADD5F5AB81 CRC64;
     MEMQSEFDRL LFFEHARKSA ETTYAQNPLD ADNLTRWGGA LLELSQFQPV AESKQMISDA
     TSKLEEALTV NPEKHDALWC LGNAHTSHVF LTPDMDEAKV YFEKATQCFQ QAFDADPSND
     LYRKSLEVTA KAPELHMEIH RHGPMQQTMA AEPSTSTSTK SSKKTKSSDL KYDIFGWVIL
     AVGIVAWVGF AKSNMPPPPP PPPQ