TOM20_YEAST
ID TOM20_YEAST Reviewed; 183 AA.
AC P35180; D6VUL4;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 187.
DE RecName: Full=Mitochondrial import receptor subunit TOM20;
DE AltName: Full=Mitochondrial 20 kDa outer membrane protein;
DE AltName: Full=Protein MAS20;
DE AltName: Full=Translocase of outer membrane 20 kDa subunit;
GN Name=TOM20; Synonyms=MAS20, MOM19; OrderedLocusNames=YGR082W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8223428; DOI=10.1002/j.1460-2075.1993.tb06095.x;
RA Ramage L., Junne T., Hahne K., Lithgow T., Schatz G.;
RT "Functional cooperation of mitochondrial protein import receptors in
RT yeast.";
RL EMBO J. 12:4115-4123(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8132642; DOI=10.1016/s0021-9258(17)37074-6;
RA Moczko M., Ehmann B., Gaertner F., Hoenlinger A., Schaefer E., Pfanner N.;
RT "Deletion of the receptor MOM19 strongly impairs import of cleavable
RT preproteins into Saccharomyces cerevisiae mitochondria.";
RL J. Biol. Chem. 269:9045-9051(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP IDENTIFICATION IN THE TOM COMPLEX.
RX PubMed=9774667; DOI=10.1128/mcb.18.11.6515;
RA Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.;
RT "Preprotein translocase of the outer mitochondrial membrane: molecular
RT dissection and assembly of the general import pore complex.";
RL Mol. Cell. Biol. 18:6515-6524(1998).
RN [7]
RP INTERACTION WITH TOM22.
RX PubMed=10519552; DOI=10.1038/46802;
RA van Wilpe S., Ryan M.T., Hill K., Maarse A.C., Meisinger C., Brix J.,
RA Dekker P.J.T., Moczko M., Wagner R., Meijer M., Guiard B., Hoenlinger A.,
RA Pfanner N.;
RT "Tom22 is a multifunctional organizer of the mitochondrial preprotein
RT translocase.";
RL Nature 401:485-489(1999).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-172, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Central component of the TOM (translocase of outer membrane)
CC receptor complex responsible for the recognition and translocation of
CC cytosolically synthesized mitochondrial preproteins. Together with
CC TOM22 functions as the transit peptide receptor at the surface of the
CC mitochondrion outer membrane and facilitates the movement of
CC preproteins into the TOM40 translocation pore.
CC -!- SUBUNIT: Forms part of the preprotein translocase complex of the outer
CC mitochondrial membrane (TOM complex) which consists of at least 7
CC different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70).
CC In the complex, interacts with TOM22. {ECO:0000269|PubMed:10519552,
CC ECO:0000269|PubMed:9774667}.
CC -!- INTERACTION:
CC P35180; P49334: TOM22; NbExp=4; IntAct=EBI-12522, EBI-12527;
CC P35180; P23644: TOM40; NbExp=3; IntAct=EBI-12522, EBI-12539;
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000305};
CC Single-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 5680 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the Tom20 family. {ECO:0000305}.
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DR EMBL; X76139; CAA53742.1; -; Genomic_DNA.
DR EMBL; X75319; CAA53068.1; -; mRNA.
DR EMBL; Z72867; CAA97084.1; -; Genomic_DNA.
DR EMBL; AY557775; AAS56101.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08175.1; -; Genomic_DNA.
DR PIR; S39568; S39568.
DR RefSeq; NP_011596.1; NM_001181211.1.
DR AlphaFoldDB; P35180; -.
DR SMR; P35180; -.
DR BioGRID; 33324; 102.
DR ComplexPortal; CPX-474; TOM40 mitochondrial outer membrane translocase holocomplex.
DR DIP; DIP-2539N; -.
DR IntAct; P35180; 14.
DR MINT; P35180; -.
DR STRING; 4932.YGR082W; -.
DR TCDB; 3.A.8.1.1; the mitochondrial protein translocase (mpt) family.
DR iPTMnet; P35180; -.
DR MaxQB; P35180; -.
DR PaxDb; P35180; -.
DR PRIDE; P35180; -.
DR EnsemblFungi; YGR082W_mRNA; YGR082W; YGR082W.
DR GeneID; 852973; -.
DR KEGG; sce:YGR082W; -.
DR SGD; S000003314; TOM20.
DR VEuPathDB; FungiDB:YGR082W; -.
DR eggNOG; KOG4056; Eukaryota.
DR GeneTree; ENSGT00390000011698; -.
DR HOGENOM; CLU_090411_0_0_1; -.
DR InParanoid; P35180; -.
DR OMA; VGYCFYF; -.
DR BioCyc; YEAST:G3O-30794-MON; -.
DR PRO; PR:P35180; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P35180; protein.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:ComplexPortal.
DR GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0030943; F:mitochondrion targeting sequence binding; IDA:SGD.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IMP:SGD.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IDA:ComplexPortal.
DR GO; GO:0016031; P:tRNA import into mitochondrion; IMP:SGD.
DR Gene3D; 1.20.960.10; -; 1.
DR InterPro; IPR002056; MAS20.
DR InterPro; IPR023392; Tom20_dom_sf.
DR PANTHER; PTHR12430; PTHR12430; 1.
DR Pfam; PF02064; MAS20; 1.
DR PIRSF; PIRSF037707; MAS20_rcpt; 1.
DR PRINTS; PR00351; OM20RECEPTOR.
DR SUPFAM; SSF47157; SSF47157; 1.
DR TIGRFAMs; TIGR00985; 3a0801s04tom; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..183
FT /note="Mitochondrial import receptor subunit TOM20"
FT /id="PRO_0000051550"
FT TOPO_DOM 1..8
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..183
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 172
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
SQ SEQUENCE 183 AA; 20317 MW; 9F76FE6010278410 CRC64;
MSQSNPILRG LAITTAIAAL SATGYAIYFD YQRRNSPQFR KVLRQRAKEQ AKMEEQAKTH
AKEVKLQKVT EFLSMELAKD PIPSDPSERE ATFTTNVENG ERLSMQQGKE LEAASKFYKA
LTVYPQPADL LGIYQRSIPE AIYEYIILMI AILPPANVAS FVKGVVGSKA ESDAVAEAND
IDD
