TOM2A_ARATH
ID TOM2A_ARATH Reviewed; 280 AA.
AC Q9C5W7; Q56Z79; Q9LQL8;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Tobamovirus multiplication protein 2A;
DE Short=AtTOM2A;
GN Name=TOM2A; OrderedLocusNames=At1g32400; ORFNames=F5D14.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, INTERACTION
RP WITH TOM1, SUBUNIT, AND TOPOLOGY.
RX PubMed=12514139; DOI=10.1093/emboj/cdg034;
RA Tsujimoto Y., Numaga T., Ohshima K., Yano M.A., Ohsawa R., Goto D.B.,
RA Naito S., Ishikawa M.;
RT "Arabidopsis TOBAMOVIRUS MULTIPLICATION (TOM) 2 locus encodes a
RT transmembrane protein that interacts with TOM1.";
RL EMBO J. 22:335-343(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 186-280.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9568044; DOI=10.1006/viro.1998.9078;
RA Ohshima K., Taniyama T., Yamanaka T., Ishikawa M., Naito S.;
RT "Isolation of a mutant of Arabidopsis thaliana carrying two simultaneous
RT mutations affecting tobacco mosaic virus multiplication within a single
RT cell.";
RL Virology 243:472-481(1998).
RN [8]
RP SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=12514140; DOI=10.1093/emboj/cdg033;
RA Hagiwara Y., Komoda K., Yamanaka T., Tamai A., Meshi T., Funada R.,
RA Tsuchiya T., Naito S., Ishikawa M.;
RT "Subcellular localization of host and viral proteins associated with
RT tobamovirus RNA replication.";
RL EMBO J. 22:344-353(2003).
RN [9]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=18474569; DOI=10.1099/vir.0.2008/000539-0;
RA Fujisaki K., Kobayashi S., Tsujimoto Y., Naito S., Ishikawa M.;
RT "Analysis of tobamovirus multiplication in Arabidopsis thaliana mutants
RT defective in TOM2A homologues.";
RL J. Gen. Virol. 89:1519-1524(2008).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=19245862; DOI=10.1016/j.jprot.2009.02.004;
RA Jones A.M.E., MacLean D., Studholme D.J., Serna-Sanz A., Andreasson E.,
RA Rathjen J.P., Peck S.C.;
RT "Phosphoproteomic analysis of nuclei-enriched fractions from Arabidopsis
RT thaliana.";
RL J. Proteomics 72:439-451(2009).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196 AND SER-233, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Necessary for the efficient intracellular multiplication of
CC tobamoviruses, being a component of the replication complex.
CC {ECO:0000269|PubMed:12514139, ECO:0000269|PubMed:18474569,
CC ECO:0000269|PubMed:9568044}.
CC -!- SUBUNIT: Homodimer. Constituent of tobamovirus replication complex.
CC Interacts with TOM1. {ECO:0000269|PubMed:12514139}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:12514140};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in rosette leaves.
CC {ECO:0000269|PubMed:18474569}.
CC -!- DISRUPTION PHENOTYPE: Reduced efficiency of intracellular
CC multiplication of tobamoviruses (e.g. crucifer strain TMV-Cg),
CC characterized by a reduced amplification of TMV-related RNAs.
CC {ECO:0000269|PubMed:12514139, ECO:0000269|PubMed:18474569,
CC ECO:0000269|PubMed:9568044}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF81337.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB085684; BAC24019.1; -; mRNA.
DR EMBL; AC007767; AAF81337.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31476.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31477.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31478.1; -; Genomic_DNA.
DR EMBL; AF334723; AAG50101.1; -; mRNA.
DR EMBL; AF385687; AAK60280.1; -; mRNA.
DR EMBL; AY088560; AAM66092.1; -; mRNA.
DR EMBL; AK221090; BAD94952.1; -; mRNA.
DR PIR; A86449; A86449.
DR RefSeq; NP_001031127.1; NM_001036050.2.
DR RefSeq; NP_564399.1; NM_102974.5.
DR RefSeq; NP_973952.1; NM_202223.3.
DR AlphaFoldDB; Q9C5W7; -.
DR BioGRID; 25367; 3.
DR IntAct; Q9C5W7; 1.
DR STRING; 3702.AT1G32400.3; -.
DR iPTMnet; Q9C5W7; -.
DR SwissPalm; Q9C5W7; -.
DR PaxDb; Q9C5W7; -.
DR PRIDE; Q9C5W7; -.
DR ProMEX; Q9C5W7; -.
DR ProteomicsDB; 234322; -.
DR EnsemblPlants; AT1G32400.1; AT1G32400.1; AT1G32400.
DR EnsemblPlants; AT1G32400.2; AT1G32400.2; AT1G32400.
DR EnsemblPlants; AT1G32400.3; AT1G32400.3; AT1G32400.
DR GeneID; 840133; -.
DR Gramene; AT1G32400.1; AT1G32400.1; AT1G32400.
DR Gramene; AT1G32400.2; AT1G32400.2; AT1G32400.
DR Gramene; AT1G32400.3; AT1G32400.3; AT1G32400.
DR KEGG; ath:AT1G32400; -.
DR Araport; AT1G32400; -.
DR TAIR; locus:2033718; AT1G32400.
DR eggNOG; ENOG502QRDH; Eukaryota.
DR HOGENOM; CLU_055730_0_0_1; -.
DR InParanoid; Q9C5W7; -.
DR OMA; NICCLSC; -.
DR OrthoDB; 1180379at2759; -.
DR PhylomeDB; Q9C5W7; -.
DR PRO; PR:Q9C5W7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C5W7; baseline and differential.
DR Genevisible; Q9C5W7; AT.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:UniProtKB.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0043621; F:protein self-association; IDA:UniProtKB.
DR GO; GO:0046786; P:viral replication complex formation and maintenance; IMP:TAIR.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Vacuole.
FT CHAIN 1..280
FT /note="Tobamovirus multiplication protein 2A"
FT /id="PRO_0000423674"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..78
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..113
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..162
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 184..280
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 258..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 196
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT MOD_RES 233
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
SQ SEQUENCE 280 AA; 31556 MW; 227C8FE0658A8C0C CRC64;
MACRGCLECL LKLLNFLLAV AGLGMIGYGI YLFVEYKRVT DNSVTFDLTN GDQSYVSFGR
PILMAVSLSS NIFDNLPKAW FIYLFIGIGV ALFVISCCGC VGTCSRSVCC LSCYSLLLIL
LILVELGFAA FIFFDNSWRD ELPSDRTGNF DTIYNFLREN WKIVRWVALG AVVFEALLFL
LALMVRAANT PAEYDSDDEY LAPRQQIRQP FINRQAAPVT GVPVAPTLDQ RPSRSDPWSA
RMREKYGLDT SEFTYNPSES HRFQQMPAQP NEEKGRCTIM
