TOM2B_ARATH
ID TOM2B_ARATH Reviewed; 122 AA.
AC Q8H960; F4ICP0; Q682B7; Q8L9F1;
DT 16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Tobamovirus multiplication protein 2B;
DE Short=AtTOM2B;
GN Name=TOM2B; Synonyms=TTM1; OrderedLocusNames=At1g32370; ORFNames=F5D14.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=12514139; DOI=10.1093/emboj/cdg034;
RA Tsujimoto Y., Numaga T., Ohshima K., Yano M.A., Ohsawa R., Goto D.B.,
RA Naito S., Ishikawa M.;
RT "Arabidopsis TOBAMOVIRUS MULTIPLICATION (TOM) 2 locus encodes a
RT transmembrane protein that interacts with TOM1.";
RL EMBO J. 22:335-343(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9568044; DOI=10.1006/viro.1998.9078;
RA Ohshima K., Taniyama T., Yamanaka T., Ishikawa M., Naito S.;
RT "Isolation of a mutant of Arabidopsis thaliana carrying two simultaneous
RT mutations affecting tobacco mosaic virus multiplication within a single
RT cell.";
RL Virology 243:472-481(1998).
CC -!- FUNCTION: Necessary for the efficient intracellular multiplication of
CC tobamoviruses. {ECO:0000269|PubMed:12514139,
CC ECO:0000269|PubMed:9568044}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q8H960-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8H960-2; Sequence=VSP_053253;
CC Name=3;
CC IsoId=Q8H960-3; Sequence=VSP_053254;
CC Name=4;
CC IsoId=Q8H960-4; Sequence=VSP_053255;
CC -!- DISRUPTION PHENOTYPE: Reduced efficiency of intracellular
CC multiplication of tobamoviruses (e.g. crucifer strain TMV-Cg).
CC {ECO:0000269|PubMed:12514139, ECO:0000269|PubMed:9568044}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX817937; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB085685; BAC24020.2; -; mRNA.
DR EMBL; AC007767; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE31470.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31471.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31472.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31473.1; -; Genomic_DNA.
DR EMBL; BX817937; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AK175450; BAD43213.1; -; mRNA.
DR EMBL; BT024563; ABD38902.1; -; mRNA.
DR EMBL; AY088467; AAM66003.1; -; mRNA.
DR RefSeq; NP_001031126.1; NM_001036049.2. [Q8H960-3]
DR RefSeq; NP_564397.1; NM_102970.3. [Q8H960-2]
DR RefSeq; NP_849740.1; NM_179409.3. [Q8H960-1]
DR RefSeq; NP_973951.1; NM_202222.3. [Q8H960-4]
DR AlphaFoldDB; Q8H960; -.
DR SMR; Q8H960; -.
DR STRING; 3702.AT1G32370.2; -.
DR PaxDb; Q8H960; -.
DR PRIDE; Q8H960; -.
DR ProteomicsDB; 232436; -. [Q8H960-1]
DR EnsemblPlants; AT1G32370.1; AT1G32370.1; AT1G32370. [Q8H960-1]
DR EnsemblPlants; AT1G32370.2; AT1G32370.2; AT1G32370. [Q8H960-2]
DR EnsemblPlants; AT1G32370.3; AT1G32370.3; AT1G32370. [Q8H960-4]
DR EnsemblPlants; AT1G32370.4; AT1G32370.4; AT1G32370. [Q8H960-3]
DR GeneID; 840129; -.
DR Gramene; AT1G32370.1; AT1G32370.1; AT1G32370. [Q8H960-1]
DR Gramene; AT1G32370.2; AT1G32370.2; AT1G32370. [Q8H960-2]
DR Gramene; AT1G32370.3; AT1G32370.3; AT1G32370. [Q8H960-4]
DR Gramene; AT1G32370.4; AT1G32370.4; AT1G32370. [Q8H960-3]
DR KEGG; ath:AT1G32370; -.
DR Araport; AT1G32370; -.
DR TAIR; locus:2033781; AT1G32370.
DR eggNOG; ENOG502S1XW; Eukaryota.
DR HOGENOM; CLU_138224_0_0_1; -.
DR OMA; MLEQMPR; -.
DR OrthoDB; 1602415at2759; -.
DR PRO; PR:Q8H960; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8H960; baseline and differential.
DR Genevisible; Q8H960; AT.
DR GO; GO:0046786; P:viral replication complex formation and maintenance; IMP:TAIR.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome.
FT CHAIN 1..122
FT /note="Tobamovirus multiplication protein 2B"
FT /id="PRO_0000423675"
FT VAR_SEQ 122
FT /note="V -> VQMTSSDLQR (in isoform 2)"
FT /evidence="ECO:0000303|Ref.7"
FT /id="VSP_053253"
FT VAR_SEQ 122
FT /note="V -> DN (in isoform 3)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_053254"
FT VAR_SEQ 122
FT /note="V -> TNDK (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_053255"
SQ SEQUENCE 122 AA; 12795 MW; 526FAE68B2FDBCB9 CRC64;
MATASENSRG ISGGDRTAKA VVADQISQAV NSTANLLHLM RQSSSSQAQL AKLPKNLLAK
ASLTKATGQA LAQLPQVISS LDAHIESGLH SGVHLNTVTQ LLENMESTQL RALRQSNISP
VV
