ID   TOM3_TOBAC              Reviewed;         294 AA.
AC   Q402F3;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Tobamovirus multiplication protein 3;
DE            Short=NtTOM3;
GN   Name=TOM3;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   BIOTECHNOLOGY.
RC   STRAIN=cv. Samsun;
RX   PubMed=16081069; DOI=10.1016/j.febslet.2005.07.021;
RA   Asano M., Satoh R., Mochizuki A., Tsuda S., Yamanaka T., Nishiguchi M.,
RA   Hirai K., Meshi T., Naito S., Ishikawa M.;
RT   "Tobamovirus-resistant tobacco generated by RNA interference directed
RT   against host genes.";
RL   FEBS Lett. 579:4479-4484(2005).
CC   -!- FUNCTION: Necessary for the efficient intracellular multiplication of
CC       tobamoviruses, probably being a membrane anchor promoting the formation
CC       of the replication complex. {ECO:0000269|PubMed:16081069}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Reduced efficiency of intracellular
CC       multiplication of tobamoviruses (e.g. TMV-L, ToMV, PMMoV, and TMGMV),
CC       characterized by a reduced accumulation of viral coat protein (CP) and
CC       reduced amplification of TMV-related RNAs.
CC       {ECO:0000269|PubMed:16081069}.
CC   -!- BIOTECHNOLOGY: TOM3 inhibition via RNA interference constitutes a
CC       useful method for generating tobamovirus-resistant plants.
CC       {ECO:0000269|PubMed:16081069}.
CC   -!- SIMILARITY: Belongs to the plant tobamovirus multiplication TOM1
CC       protein family. {ECO:0000305}.
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DR   EMBL; AB193040; BAE43837.1; -; mRNA.
DR   RefSeq; NP_001311968.1; NM_001325039.1.
DR   AlphaFoldDB; Q402F3; -.
DR   SMR; Q402F3; -.
DR   GeneID; 107769903; -.
DR   KEGG; nta:107769903; -.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009705; C:plant-type vacuole membrane; ISS:UniProtKB.
DR   GO; GO:0046786; P:viral replication complex formation and maintenance; IMP:UniProtKB.
DR   InterPro; IPR040226; THH1/TOM1/TOM3.
DR   InterPro; IPR009457; THH1/TOM1/TOM3_dom.
DR   PANTHER; PTHR31142; PTHR31142; 1.
DR   Pfam; PF06454; DUF1084; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Vacuole.
FT   CHAIN           1..294
FT                   /note="Tobamovirus multiplication protein 3"
FT                   /id="PRO_0000423673"
FT   TOPO_DOM        1..39
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        40..60
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        61..75
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        76..94
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        95..108
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        130..147
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..172
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        173..193
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        194..220
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        242..256
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        257..277
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        278..294
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        31
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   294 AA;  34023 MW;  9838BB756FD7C798 CRC64;
     MGRAEMVVGP SAAAAAYHLK NEAISWWEEV NRSRAWQDRL FHVLAILYGI VSAVALVQLI
     RIQMRVPEYG WTTQKVFHFL NFLVNGVRSL VFAFRRDVQK LHPEIVQHIL LDMPSLAFFT
     TYALLVLFWA EIYYQARAVS TDGLRPSFFT INGVVYVIQI ILWLIIWWKP VRVLVILSKM
     FFAGVSLFAA LGFLLYGGRL FLMLQRFPVE SKGRRKKLQE VGYVTTICFS CFLIRCVMMC
     FNAFNKAADL DVLDHPILNL IYYLLVEILP SSLVLFILRK LPPKRGITQY HPIR