TOM5_YEAST
ID TOM5_YEAST Reviewed; 50 AA.
AC P80967; D6W4D0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Mitochondrial import receptor subunit TOM5;
DE AltName: Full=Translocase of outer membrane 5 kDa subunit;
GN Name=TOM5; Synonyms=MOM8A; OrderedLocusNames=YPR133W-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION, FUNCTION, AND PROTEIN SEQUENCE OF 1-21.
RX PubMed=9217162; DOI=10.1038/40663;
RA Dietmeier K., Hoenlinger A., Boemer U., Dekker P.J.T., Eckerskorn C.,
RA Lottspeich F., Kuebrich M., Pfanner N.;
RT "Tom5 functionally links mitochondrial preprotein receptors to the general
RT import pore.";
RL Nature 388:195-200(1997).
RN [5]
RP IDENTIFICATION IN THE TOM COMPLEX.
RX PubMed=9774667; DOI=10.1128/mcb.18.11.6515;
RA Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.;
RT "Preprotein translocase of the outer mitochondrial membrane: molecular
RT dissection and assembly of the general import pore complex.";
RL Mol. Cell. Biol. 18:6515-6524(1998).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the TOM (translocase of outer membrane) receptor
CC complex responsible for the recognition and translocation of
CC cytosolically synthesized mitochondrial preproteins. TOM5 is involved
CC in insertion of preproteins into the TOM40 translocation pore.
CC {ECO:0000269|PubMed:9217162}.
CC -!- SUBUNIT: Forms part of the TOM (translocase of outer membrane) complex
CC that consists of at least 7 different proteins (TOM5, TOM6, TOM7,
CC TOM20, TOM22, TOM40 and TOM70). {ECO:0000269|PubMed:9774667}.
CC -!- INTERACTION:
CC P80967; P25574: EMC1; NbExp=2; IntAct=EBI-12501, EBI-21744;
CC P80967; P47133: EMC2; NbExp=3; IntAct=EBI-12501, EBI-25568;
CC P80967; P40540: EMC5; NbExp=2; IntAct=EBI-12501, EBI-24977;
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane {ECO:0000305};
CC Single-pass membrane protein {ECO:0000305}. Note=Outer membrane-
CC anchored. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the Tom5 family. {ECO:0000305}.
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DR EMBL; U40829; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AY558369; AAS56695.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11546.1; -; Genomic_DNA.
DR PIR; S77712; S77712.
DR RefSeq; NP_015459.1; NM_001184307.1.
DR PDB; 6JNF; EM; 3.81 A; D/I=1-50.
DR PDB; 6UCU; EM; 3.06 A; C/K=1-50.
DR PDB; 6UCV; EM; 4.10 A; C/K/c/k=1-50.
DR PDB; 7E4I; EM; 3.05 A; E=1-50.
DR PDBsum; 6JNF; -.
DR PDBsum; 6UCU; -.
DR PDBsum; 6UCV; -.
DR PDBsum; 7E4I; -.
DR AlphaFoldDB; P80967; -.
DR SMR; P80967; -.
DR BioGRID; 36300; 53.
DR ComplexPortal; CPX-473; TOM40 mitochondrial outer membrane translocase core complex.
DR ComplexPortal; CPX-474; TOM40 mitochondrial outer membrane translocase holocomplex.
DR DIP; DIP-7358N; -.
DR IntAct; P80967; 10.
DR STRING; 4932.YPR133W-A; -.
DR TCDB; 3.A.8.1.1; the mitochondrial protein translocase (mpt) family.
DR MaxQB; P80967; -.
DR PaxDb; P80967; -.
DR PRIDE; P80967; -.
DR EnsemblFungi; YPR133W-A_mRNA; YPR133W-A; YPR133W-A.
DR GeneID; 856252; -.
DR KEGG; sce:YPR133W-A; -.
DR SGD; S000006433; TOM5.
DR VEuPathDB; FungiDB:YPR133W-A; -.
DR eggNOG; ENOG502SD3F; Eukaryota.
DR HOGENOM; CLU_209440_1_1_1; -.
DR InParanoid; P80967; -.
DR OMA; SPIVWHF; -.
DR BioCyc; YEAST:G3O-34325-MON; -.
DR PRO; PR:P80967; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; IDA:SGD.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:ComplexPortal.
DR GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0070096; P:mitochondrial outer membrane translocase complex assembly; IDA:SGD.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IDA:ComplexPortal.
DR GO; GO:0006626; P:protein targeting to mitochondrion; IMP:SGD.
DR GO; GO:0071806; P:protein transmembrane transport; IEA:GOC.
DR InterPro; IPR019603; Tom5.
DR Pfam; PF10642; Tom5; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Protein transport; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..50
FT /note="Mitochondrial import receptor subunit TOM5"
FT /id="PRO_0000218264"
FT TOPO_DOM 1..26
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..45
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 14..48
FT /evidence="ECO:0007829|PDB:7E4I"
SQ SEQUENCE 50 AA; 5985 MW; E1419ED5FEC45E14 CRC64;
MFGLPQQEVS EEEKRAHQEQ TEKTLKQAAY VAAFLWVSPM IWHLVKKQWK
