TOM6_YEAST
ID TOM6_YEAST Reviewed; 61 AA.
AC P33448; D6W2B1;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Mitochondrial import receptor subunit TOM6;
DE AltName: Full=Mitochondrial import site protein ISP6;
DE AltName: Full=Translocase of outer membrane 6 kDa subunit;
GN Name=TOM6; Synonyms=ISP6; OrderedLocusNames=YOR045W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8344244; DOI=10.1002/j.1460-2075.1993.tb05971.x;
RA Kassenbrock C.K., Cao W., Douglas M.G.;
RT "Genetic and biochemical characterization of ISP6, a small mitochondrial
RT outer membrane protein associated with the protein translocation complex.";
RL EMBO J. 12:3023-3034(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION IN THE TOM COMPLEX.
RX PubMed=9774667; DOI=10.1128/mcb.18.11.6515;
RA Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.;
RT "Preprotein translocase of the outer mitochondrial membrane: molecular
RT dissection and assembly of the general import pore complex.";
RL Mol. Cell. Biol. 18:6515-6524(1998).
RN [6]
RP FUNCTION.
RX PubMed=11276259; DOI=10.1038/86253;
RA Model K., Meisinger C., Prinz T., Wiedemann N., Truscott K.N., Pfanner N.,
RA Ryan M.T.;
RT "Multistep assembly of the protein import channel of the mitochondrial
RT outer membrane.";
RL Nat. Struct. Biol. 8:361-370(2001).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the TOM (translocase of outer membrane) receptor
CC complex responsible for the recognition and translocation of
CC cytosolically synthesized mitochondrial preproteins. TOM6 is involved
CC in assembly and stability of the TOM complex.
CC {ECO:0000269|PubMed:11276259}.
CC -!- SUBUNIT: Forms part of the TOM (translocase of outer membrane) complex
CC that consists of at least 7 different proteins (TOM5, TOM6, TOM7,
CC TOM20, TOM22, TOM40 and TOM70). {ECO:0000269|PubMed:9774667}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass
CC membrane protein.
CC -!- SIMILARITY: Belongs to the Tom6 family. {ECO:0000305}.
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DR EMBL; Z22815; CAA80469.1; -; Genomic_DNA.
DR EMBL; Z74953; CAA99236.1; -; Genomic_DNA.
DR EMBL; AY693051; AAT93070.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10827.1; -; Genomic_DNA.
DR PIR; S35320; S35320.
DR RefSeq; NP_014688.1; NM_001183464.1.
DR PDB; 6JNF; EM; 3.81 A; E/J=1-61.
DR PDB; 6UCU; EM; 3.06 A; D/L=1-61.
DR PDB; 6UCV; EM; 4.10 A; D/L/d/l=1-61.
DR PDB; 7E4I; EM; 3.05 A; F=2-61.
DR PDBsum; 6JNF; -.
DR PDBsum; 6UCU; -.
DR PDBsum; 6UCV; -.
DR PDBsum; 7E4I; -.
DR AlphaFoldDB; P33448; -.
DR SMR; P33448; -.
DR BioGRID; 34446; 162.
DR ComplexPortal; CPX-473; TOM40 mitochondrial outer membrane translocase core complex.
DR ComplexPortal; CPX-474; TOM40 mitochondrial outer membrane translocase holocomplex.
DR DIP; DIP-3835N; -.
DR IntAct; P33448; 3.
DR STRING; 4932.YOR045W; -.
DR TCDB; 3.A.8.1.1; the mitochondrial protein translocase (mpt) family.
DR iPTMnet; P33448; -.
DR MaxQB; P33448; -.
DR PaxDb; P33448; -.
DR PRIDE; P33448; -.
DR EnsemblFungi; YOR045W_mRNA; YOR045W; YOR045W.
DR GeneID; 854210; -.
DR KEGG; sce:YOR045W; -.
DR SGD; S000005571; TOM6.
DR VEuPathDB; FungiDB:YOR045W; -.
DR eggNOG; ENOG502S7PB; Eukaryota.
DR HOGENOM; CLU_207409_0_0_1; -.
DR InParanoid; P33448; -.
DR OMA; LMDMMAP; -.
DR BioCyc; YEAST:G3O-33589-MON; -.
DR PRO; PR:P33448; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; P33448; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:ComplexPortal.
DR GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IMP:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0070096; P:mitochondrial outer membrane translocase complex assembly; IDA:SGD.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IMP:SGD.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IDA:ComplexPortal.
DR InterPro; IPR020266; Tom6.
DR Pfam; PF17112; Tom6; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..61
FT /note="Mitochondrial import receptor subunit TOM6"
FT /id="PRO_0000210820"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..61
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT HELIX 31..50
FT /evidence="ECO:0007829|PDB:7E4I"
FT HELIX 52..57
FT /evidence="ECO:0007829|PDB:7E4I"
SQ SEQUENCE 61 AA; 6408 MW; EE3F295BC0FE7240 CRC64;
MDGMFAMPGA AAGAASPQQP KSRFQAFKES PLYTIALNGA FFVAGVAFIQ SPLMDMLAPQ
L
