TOM70_SCHPO
ID TOM70_SCHPO Reviewed; 625 AA.
AC O14217;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Probable mitochondrial import receptor subunit tom70;
DE AltName: Full=Translocase of outer membrane 40 kDa subunit;
GN Name=tom70; ORFNames=SPAC6B12.12;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-128 AND SER-129, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
RN [3]
RP DISRUPTION PHENOTYPE.
RX PubMed=33138913; DOI=10.7554/elife.61245;
RA Fukuda T., Ebi Y., Saigusa T., Furukawa K., Yamashita S.I., Inoue K.,
RA Kobayashi D., Yoshida Y., Kanki T.;
RT "Atg43 tethers isolation membranes to mitochondria to promote starvation-
RT induced mitophagy in fission yeast.";
RL Elife 9:61245-61245(2020).
CC -!- FUNCTION: Component of the TOM (translocase of outer membrane) receptor
CC complex responsible for the recognition and translocation of
CC cytosolically synthesized mitochondrial preproteins. Together with
CC tom20 and tom22 functions as the transit peptide receptor at the
CC surface of the mitochondrion outer membrane and facilitates the
CC movement of preproteins into the tom40 translocation pore.
CC {ECO:0000250|UniProtKB:P07213}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000250|UniProtKB:P07213}; Single-pass membrane protein
CC {ECO:0000250|UniProtKB:P07213}.
CC -!- DISRUPTION PHENOTYPE: Abnormal mitophagy during nitrogen starvation.
CC {ECO:0000269|PubMed:33138913}.
CC -!- SIMILARITY: Belongs to the Tom70 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB11072.1; -; Genomic_DNA.
DR PIR; T39019; T39019.
DR RefSeq; NP_593767.1; NM_001019197.2.
DR AlphaFoldDB; O14217; -.
DR SMR; O14217; -.
DR BioGRID; 279728; 488.
DR STRING; 4896.SPAC6B12.12.1; -.
DR iPTMnet; O14217; -.
DR MaxQB; O14217; -.
DR PaxDb; O14217; -.
DR PRIDE; O14217; -.
DR EnsemblFungi; SPAC6B12.12.1; SPAC6B12.12.1:pep; SPAC6B12.12.
DR GeneID; 2543304; -.
DR KEGG; spo:SPAC6B12.12; -.
DR PomBase; SPAC6B12.12; tom70.
DR VEuPathDB; FungiDB:SPAC6B12.12; -.
DR eggNOG; KOG0547; Eukaryota.
DR HOGENOM; CLU_017516_0_1_1; -.
DR InParanoid; O14217; -.
DR OMA; HEATKDM; -.
DR PhylomeDB; O14217; -.
DR PRO; PR:O14217; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0031307; C:integral component of mitochondrial outer membrane; IBA:GO_Central.
DR GO; GO:0005742; C:mitochondrial outer membrane translocase complex; ISO:PomBase.
DR GO; GO:0005739; C:mitochondrion; HDA:PomBase.
DR GO; GO:0030943; F:mitochondrion targeting sequence binding; ISO:PomBase.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; ISO:PomBase.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF13176; TPR_7; 1.
DR SMART; SM00028; TPR; 8.
DR SUPFAM; SSF48452; SSF48452; 3.
DR PROSITE; PS50005; TPR; 7.
DR PROSITE; PS50293; TPR_REGION; 3.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein;
KW Reference proteome; Repeat; TPR repeat; Transmembrane; Transmembrane helix.
FT CHAIN 1..625
FT /note="Probable mitochondrial import receptor subunit
FT tom70"
FT /id="PRO_0000106339"
FT TOPO_DOM 1..31
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..625
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 151..183
FT /note="TPR 1"
FT REPEAT 184..217
FT /note="TPR 2"
FT REPEAT 219..251
FT /note="TPR 3"
FT REPEAT 347..380
FT /note="TPR 4"
FT REPEAT 415..448
FT /note="TPR 5"
FT REPEAT 450..482
FT /note="TPR 6"
FT REPEAT 483..516
FT /note="TPR 7"
FT REPEAT 524..557
FT /note="TPR 8"
FT REPEAT 558..591
FT /note="TPR 9"
FT REGION 56..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 128
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 129
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
SQ SEQUENCE 625 AA; 69517 MW; FDA9956FE85D1241 CRC64;
MSGISNAENG KAIVSSGVLN SVSAFVRRHR TFVYTIGAVA VFATVGGVYH VQQKKASHKR
SKKLKAHQDK AESKVNEGKN EAAKVVKEED LKSSETGKDV ETAAAAAAAA KKKKKNKKKV
KAKKVGDSSV DKIATEESVK SMTKEERAKL AAELKTLGNK AYGQKEYANA IDYYTQAITC
SHDPIFFSNR AACYAAIGDF EQVIKDTSEA LSLDSSYVKA LNRRSAAYEQ LGKLDEALMD
STVSCIFDGF ANESMTATVE RLLKKVAEKK SSALLKERPP KLPAASFIQT YLDSFHAQPK
PLFDNKFDGD AALAEAYEYL EKGEYQLSYD KAKESCLGSF SSPSVNARTH NLVGTFKFVS
GDSKGSMENF NAAIKLDRKF IQPYIRLSAA YLDENDNEKM WKVLNDAESV DKTDSDLYYH
RAQVRFVSGE FAEAISDYQK SIALDDSFIY SHIQLGVAQY KTHAIAESMK TFEDCKKRFP
NSSEVYNYFG EILLDQQKFD DAVKNFDHAI ELEKREHLTI MSAMPLINKA LAVFQWKKDI
SQAENLCRQA LSADPECDIA IASMAQFLLQ QGKAREALEY FEKSAQLART ESEMVNAFSY
AEATRTQIAL TEKYPQLVGR LSNPM
