TOM7_YEAST
ID TOM7_YEAST Reviewed; 60 AA.
AC P53507; D6W1A9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Mitochondrial import receptor subunit TOM7;
DE AltName: Full=Translocase of outer membrane 7 kDa subunit;
GN Name=TOM7; Synonyms=MOM7; OrderedLocusNames=YNL070W; ORFNames=N2378;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-12.
RX PubMed=8641278; DOI=10.1002/j.1460-2075.1996.tb00566.x;
RA Hoenlinger A., Boemer U., Alconada A., Eckerskorn C., Lottspeich F.,
RA Dietmeier K., Pfanner N.;
RT "Tom7 modulates the dynamics of the mitochondrial outer membrane
RT translocase and plays a pathway-related role in protein import.";
RL EMBO J. 15:2125-2137(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8701611;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<391::aid-yea921>3.0.co;2-n;
RA Poehlmann R., Philippsen P.;
RT "Sequencing a cosmid clone of Saccharomyces cerevisiae chromosome XIV
RT reveals 12 new open reading frames (ORFs) and an ancient duplication of six
RT ORFs.";
RL Yeast 12:391-402(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP IDENTIFICATION IN THE TOM COMPLEX.
RX PubMed=9774667; DOI=10.1128/mcb.18.11.6515;
RA Dekker P.J.T., Ryan M.T., Brix J., Mueller H., Hoenlinger A., Pfanner N.;
RT "Preprotein translocase of the outer mitochondrial membrane: molecular
RT dissection and assembly of the general import pore complex.";
RL Mol. Cell. Biol. 18:6515-6524(1998).
RN [7]
RP FUNCTION.
RX PubMed=11276259; DOI=10.1038/86253;
RA Model K., Meisinger C., Prinz T., Wiedemann N., Truscott K.N., Pfanner N.,
RA Ryan M.T.;
RT "Multistep assembly of the protein import channel of the mitochondrial
RT outer membrane.";
RL Nat. Struct. Biol. 8:361-370(2001).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the TOM (translocase of outer membrane) receptor
CC complex responsible for the recognition and translocation of
CC cytosolically synthesized mitochondrial preproteins. TOM7 is involved
CC in assembly and stability of the TOM complex.
CC {ECO:0000269|PubMed:11276259}.
CC -!- SUBUNIT: Forms part of the preprotein translocase complex of the outer
CC mitochondrial membrane (TOM complex) which consists of at least 7
CC different proteins (TOM5, TOM6, TOM7, TOM20, TOM22, TOM40 and TOM70).
CC {ECO:0000269|PubMed:9774667}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass
CC membrane protein.
CC -!- MISCELLANEOUS: Present with 3250 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the Tom7 family. {ECO:0000305}.
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DR EMBL; Z71346; CAA95944.1; -; Genomic_DNA.
DR EMBL; AY558519; AAS56845.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10475.1; -; Genomic_DNA.
DR PIR; S63002; S63002.
DR RefSeq; NP_014329.1; NM_001182908.1.
DR PDB; 6JNF; EM; 3.81 A; B/G=1-60.
DR PDB; 6UCU; EM; 3.06 A; E/M=1-60.
DR PDB; 6UCV; EM; 4.10 A; E/M/e/m=1-60.
DR PDBsum; 6JNF; -.
DR PDBsum; 6UCU; -.
DR PDBsum; 6UCV; -.
DR AlphaFoldDB; P53507; -.
DR SMR; P53507; -.
DR BioGRID; 35753; 175.
DR ComplexPortal; CPX-473; TOM40 mitochondrial outer membrane translocase core complex.
DR ComplexPortal; CPX-474; TOM40 mitochondrial outer membrane translocase holocomplex.
DR DIP; DIP-4364N; -.
DR IntAct; P53507; 5.
DR MINT; P53507; -.
DR STRING; 4932.YNL070W; -.
DR TCDB; 3.A.8.1.1; the mitochondrial protein translocase (mpt) family.
DR MaxQB; P53507; -.
DR PaxDb; P53507; -.
DR PRIDE; P53507; -.
DR EnsemblFungi; YNL070W_mRNA; YNL070W; YNL070W.
DR GeneID; 855654; -.
DR KEGG; sce:YNL070W; -.
DR SGD; S000005014; TOM7.
DR VEuPathDB; FungiDB:YNL070W; -.
DR eggNOG; KOG4449; Eukaryota.
DR HOGENOM; CLU_173610_1_1_1; -.
DR InParanoid; P53507; -.
DR OMA; FILYLGW; -.
DR BioCyc; YEAST:G3O-33100-MON; -.
DR PRO; PR:P53507; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53507; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005741; C:mitochondrial outer membrane; IDA:ComplexPortal.
DR GO; GO:0005742; C:mitochondrial outer membrane translocase complex; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IMP:SGD.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IDA:ComplexPortal.
DR InterPro; IPR012621; Tom7.
DR Pfam; PF08038; Tom7; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Protein transport; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8641278"
FT CHAIN 2..60
FT /note="Mitochondrial import receptor subunit TOM7"
FT /id="PRO_0000046767"
FT TOPO_DOM 2..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 20..41
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 42..60
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000250"
FT HELIX 12..42
FT /evidence="ECO:0007829|PDB:6UCU"
FT HELIX 50..54
FT /evidence="ECO:0007829|PDB:6UCU"
SQ SEQUENCE 60 AA; 6870 MW; 0EFADB326E8926E3 CRC64;
MSFLPSFILS DESKERISKI LTLTHNVAHY GWIPFVLYLG WAHTSNRPNF LNLLSPLPSV
