TOMB_ECOLI
ID TOMB_ECOLI Reviewed; 124 AA.
AC P0AAR0; P37611; P75708; Q2MBW6;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Hha toxicity modulator TomB;
DE AltName: Full=Toxin overexpression modulator in biofilms;
GN Name=tomB; Synonyms=ybaJ; OrderedLocusNames=b0461, JW0450;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 19-124.
RC STRAIN=K12 / C600 / CR34 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222;
RX PubMed=1956303; DOI=10.1111/j.1365-2958.1991.tb01902.x;
RA Nieto J.M., Carmona M., Bolland S., Jubete Y., de la Cruz F., Juarez A.;
RT "The hha gene modulates haemolysin expression in Escherichia coli.";
RL Mol. Microbiol. 5:1285-1293(1991).
RN [4]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
RN [5]
RP INDUCTION.
RC STRAIN=K12 / JM109 / ATCC 53323;
RX PubMed=14727089; DOI=10.1007/s00253-003-1517-y;
RA Ren D., Bedzyk L.A., Thomas S.M., Ye R.W., Wood T.K.;
RT "Gene expression in Escherichia coli biofilms.";
RL Appl. Microbiol. Biotechnol. 64:515-524(2004).
RN [6]
RP FUNCTION IN BIOFILM FORMATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=K12 / ATCC 25404 / DSM 5698 / NCIMB 11290;
RX PubMed=16317765; DOI=10.1002/bit.20681;
RA Barrios A.F., Zuo R., Ren D., Wood T.K.;
RT "Hha, YbaJ, and OmpA regulate Escherichia coli K12 biofilm formation and
RT conjugation plasmids abolish motility.";
RL Biotechnol. Bioeng. 93:188-200(2006).
RN [7]
RP FUNCTION, INDUCTION, DNA-BINDING, AND GENE NAME.
RC STRAIN=K12 / BW25113;
RX PubMed=18545668; DOI=10.1371/journal.pone.0002394;
RA Garcia-Contreras R., Zhang X.S., Kim Y., Wood T.K.;
RT "Protein translation and cell death: the role of rare tRNAs in biofilm
RT formation and in activating dormant phage killer genes.";
RL PLoS ONE 3:E2394-E2394(2008).
CC -!- FUNCTION: Attenuates Hha toxicity and regulates biofilm formation.
CC Binds to various coding and intergenic regions of genomic DNA.
CC {ECO:0000269|PubMed:16317765, ECO:0000269|PubMed:18545668}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expression is autoregulated (Probable). Induced during
CC biofilm formation. {ECO:0000269|PubMed:14727089,
CC ECO:0000269|PubMed:18545668, ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Deletion of hha and tomB, in the presence of a
CC conjugative plasmid (R1drd19), decreases biofilm formation, cell
CC aggregation and increases motility via flagella and motility gene
CC expression. {ECO:0000269|PubMed:16317765}.
CC -!- MISCELLANEOUS: Hha and TomB may form a type II toxin-antitoxin (TA)
CC system (PubMed:18545668). {ECO:0000305|PubMed:18545668}.
CC -!- SIMILARITY: Belongs to the TomB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=X57977; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U00096; AAC73563.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76240.1; -; Genomic_DNA.
DR EMBL; X57977; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; D64776; D64776.
DR RefSeq; NP_414994.1; NC_000913.3.
DR RefSeq; WP_000344800.1; NZ_STEB01000007.1.
DR AlphaFoldDB; P0AAR0; -.
DR SMR; P0AAR0; -.
DR BioGRID; 4259498; 27.
DR DIP; DIP-48132N; -.
DR STRING; 511145.b0461; -.
DR PaxDb; P0AAR0; -.
DR PRIDE; P0AAR0; -.
DR EnsemblBacteria; AAC73563; AAC73563; b0461.
DR EnsemblBacteria; BAE76240; BAE76240; BAE76240.
DR GeneID; 66671237; -.
DR GeneID; 945106; -.
DR KEGG; ecj:JW0450; -.
DR KEGG; eco:b0461; -.
DR PATRIC; fig|1411691.4.peg.1815; -.
DR EchoBASE; EB2326; -.
DR eggNOG; ENOG502ZC6G; Bacteria.
DR HOGENOM; CLU_164850_0_0_6; -.
DR OMA; CERVEKY; -.
DR PhylomeDB; P0AAR0; -.
DR BioCyc; EcoCyc:EG12429-MON; -.
DR PRO; PR:P0AAR0; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR GO; GO:0044010; P:single-species biofilm formation; IEP:EcoCyc.
DR InterPro; IPR019693; Biofilm_formation_reg_YbaJ.
DR Pfam; PF10757; YbaJ; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Reference proteome.
FT CHAIN 1..124
FT /note="Hha toxicity modulator TomB"
FT /id="PRO_0000168617"
SQ SEQUENCE 124 AA; 14557 MW; 5F410DFFBD29F270 CRC64;
MDEYSPKRHD IAQLKFLCET LYHDCLANLE ESNHGWVNDP TSAINLQLNE LIEHIATFAL
NYKIKYNEDN KLIEQIDEYL DDTFMLFSSY GINMQDLQKW RKSGNRLFRC FVNATKENPA
SLSC
