TONSL_CITUN
ID TONSL_CITUN Reviewed; 1307 AA.
AC A0A2H5Q1B8;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2018, sequence version 1.
DT 03-AUG-2022, entry version 14.
DE RecName: Full=Protein TONSOKU {ECO:0000303|PubMed:35298257};
DE Short=CuTSK {ECO:0000303|PubMed:35298257};
GN Name=TSK {ECO:0000303|PubMed:35298257}; ORFNames=CUMW_187040;
OS Citrus unshiu (Satsuma mandarin) (Citrus nobilis var. unshiu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=55188;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Miyagawa wase;
RX PubMed=29259619; DOI=10.3389/fgene.2017.00180;
RA Shimizu T., Tanizawa Y., Mochizuki T., Nagasaki H., Yoshioka T., Toyoda A.,
RA Fujiyama A., Kaminuma E., Nakamura Y.;
RT "Draft sequencing of the heterozygous diploid genome of Satsuma (Citrus
RT unshiu Marc.) using a hybrid assembly approach.";
RL Front. Genet. 8:180-180(2017).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (3.17 ANGSTROMS) OF 1-490 IN COMPLEX WITH HISTONE
RP H3.1, FUNCTION, AND MUTAGENESIS OF THR-70; GLN-72; ASP-166; GLU-177 AND
RP ASP-234.
RX PubMed=35298257; DOI=10.1126/science.abm5320;
RA Davarinejad H., Huang Y.C., Mermaz B., LeBlanc C., Poulet A., Thomson G.,
RA Joly V., Munoz M., Arvanitis-Vigneault A., Valsakumar D., Villarino G.,
RA Ross A., Rotstein B.H., Alarcon E.I., Brunzelle J.S., Voigt P., Dong J.,
RA Couture J.F., Jacob Y.;
RT "The histone H3.1 variant regulates TONSOKU-mediated DNA repair during
RT replication.";
RL Science 375:1281-1286(2022).
CC -!- FUNCTION: Histone reader involved in homologous recombination-mediated
CC repair of double-strand breaks (DSBs) at stalled or collapsed
CC replication forks (By similarity). Specifically recognizes and binds
CC histone H3.1 lacking methylation at 'Lys-27' (H3K27me1)
CC (PubMed:35298257). {ECO:0000250|UniProtKB:Q6Q4D0,
CC ECO:0000269|PubMed:35298257}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q6Q4D0}. Chromosome
CC {ECO:0000250|UniProtKB:Q6Q4D0}.
CC -!- SIMILARITY: Belongs to the Tonsoku family. {ECO:0000305}.
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DR EMBL; BDQV01000183; GAY58446.1; -; Genomic_DNA.
DR PDB; 7T7T; X-ray; 3.17 A; A/B=1-490.
DR PDBsum; 7T7T; -.
DR Proteomes; UP000236630; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0042393; F:histone binding; IDA:UniProtKB.
DR GO; GO:0009933; P:meristem structural organization; IEA:InterPro.
DR GO; GO:0040029; P:regulation of gene expression, epigenetic; IEA:InterPro.
DR GO; GO:0072423; P:response to DNA damage checkpoint signaling; IEA:InterPro.
DR Gene3D; 1.25.40.10; -; 3.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR044227; TONSOKU.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR47684; PTHR47684; 1.
DR Pfam; PF13181; TPR_8; 1.
DR SMART; SM00028; TPR; 6.
DR SUPFAM; SSF48452; SSF48452; 3.
DR PROSITE; PS50005; TPR; 6.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chromatin regulator; Chromosome; DNA damage; DNA repair;
KW Leucine-rich repeat; Nucleus; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..1307
FT /note="Protein TONSOKU"
FT /id="PRO_0000455986"
FT REPEAT 28..61
FT /note="TPR 7"
FT /evidence="ECO:0000255"
FT REPEAT 68..101
FT /note="TPR 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 109..149
FT /note="TPR 9"
FT /evidence="ECO:0000255"
FT REPEAT 165..198
FT /note="TPR 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 208..241
FT /note="TPR 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 248..281
FT /note="TPR 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 283..308
FT /note="TPR 13"
FT /evidence="ECO:0000255"
FT REPEAT 302..325
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 379..412
FT /note="TPR 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 419..452
FT /note="TPR 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00339"
FT REPEAT 564..587
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 739..767
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 856..879
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 907..940
FT /note="TPR 16"
FT /evidence="ECO:0000255"
FT REPEAT 995..1019
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 1079..1102
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 1245..1269
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT MUTAGEN 70
FT /note="T->R: Abolished interaction with histone H3.1."
FT /evidence="ECO:0000269|PubMed:35298257"
FT MUTAGEN 72
FT /note="Q->N: Slightly reduced interaction with histone
FT H3.1."
FT /evidence="ECO:0000269|PubMed:35298257"
FT MUTAGEN 72
FT /note="Q->S: Strongly reduced interaction with histone
FT H3.1."
FT /evidence="ECO:0000269|PubMed:35298257"
FT MUTAGEN 166
FT /note="D->R: Abolished interaction with histone H3.1."
FT /evidence="ECO:0000269|PubMed:35298257"
FT MUTAGEN 177
FT /note="E->R: Abolished interaction with histone H3.1."
FT /evidence="ECO:0000269|PubMed:35298257"
FT MUTAGEN 234
FT /note="D->R: Abolished interaction with histone H3.1."
FT /evidence="ECO:0000269|PubMed:35298257"
SQ SEQUENCE 1307 AA; 146976 MW; 7819285371730707 CRC64;
MGRDEMQMSE AKRAYRSAKE EGNRQEEARW ANVIGDILKN RGEYVEALKW FRIDYDVSVK
YLPEKHLLPT CQSLGEVYLR LEHFKDALIY QKKHLELAKD ASDLVEQQRA CTQLGRTYYE
MFLRSDDDHY SIRNAKKYFK SAMKLAQTLK ENPATSRSSF LKEYIDAHNN IGMLQMELDN
LEEAKKLLIR GLEICNEEEV SEDDDGRSRL HHNLGNVYME LRMWDKSREH IEQDIIICKK
IEHRQGEAKG YINLGELHYR VQKYDEAILC YQKALNLAQS MEDEDALASQ IDQNIETVKK
AIEVMDELKK EEQNLKKLTR NMIIAKGTSQ ERKSLLQQNA SLDCLIEKSS MIFAWLKHCE
YAKRKKRIAS ELCDKGKLSD SFLVIGESYQ KLRKFNKAIK WYTKSWEMYK SIGNLEGQAL
AKVNMGNVLD SNGDWAGALD AFQEGYRIAV EANLPSVQLS ALENMHYSHM IRFDNIEEAR
RLQHEIDKLK ESKSEDLEAY DVAKDCCSET DTEGNDHLPD VRSSACFSAE MSKSDSGRSK
TLAGLEEVED DEPLISFLRS SERLPKLKRA YVEKQNIPTE PRELTPKTLS KSTNSQQTGG
GRKRIRVVLS DDEGDIDNEE GLKGRLQKCP VEGVATFDAS RWFILLVWQD VPAADFKCTN
SSENPINVEE STCSHKFTSS NPTNQYGNAI RCTGKVFIAS DVNDDQCVTF RIDDDLIQLE
VHSCICDDKL DIESLKVELA CLYYLQLPKE KISKGLLPII QHMKYGGRSL ESFDAFKDQL
GKDIIEVSID GWVQKRLMKL YIECCKELSE APNMKLLKKL YISEVEDEVV VSECELQDIS
VTPLLNALQT HKTVAMLDLS HNLLGNGTME KLQQFFISSC QNYVDLTLDL HCNRFGPTTL
FQICECPVLF TRLGVLNLSG NRLTDACGSY LSTILKNCKV LYSLNIENCS ITSRTIQKVA
DALGAESTLA QLCIGYNSPV TGNAITNLLV KLDTLKSFSE LNLNGLKLSK PVVDRLCQLA
KTSCLTHLML GCTNLGSDGS LQLVESLFSR AQESVKLDLS YCGLESTCIH KFTASVSLVH
GILELNLGGN PIMKEGANAL ASLLMNPQCC LKVLVLSKCQ LGLAGVLQLI KALSENDTLE
ELNLADNASK ELTLQQNLSS VNSENLQPAL KTSDGVSKEV DTDQQGLCAM NTDCNDLEVA
DSEDDKIRVE SAASGFDNSC TSSCQKNSSF ECQFIQELSS AIGMAKPLQL LDLSNNGFST
QAVKTLYSAW SSRSGAGPTW KHIKEQIIHF SVEGNKCCRV KPCCRKN
