TONSL_DROME
ID TONSL_DROME Reviewed; 1405 AA.
AC Q9VSA4; Q8MT49;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Tonsoku-like protein;
GN ORFNames=CG7457;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-707; SER-709; SER-893;
RP SER-895; SER-899 AND SER-902, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Histone reader involved in homologous recombination-mediated
CC repair of double-strand breaks (DSBs) at stalled or collapsed
CC replication forks. Specifically recognizes and binds histone H3.1.
CC {ECO:0000250|UniProtKB:Q96HA7}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000250|UniProtKB:Q96HA7}. Chromosome
CC {ECO:0000250|UniProtKB:Q96HA7}.
CC -!- SIMILARITY: Belongs to the Tonsoku family. {ECO:0000305}.
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DR EMBL; AE014296; AAF50523.1; -; Genomic_DNA.
DR EMBL; AY118382; AAM48411.1; -; mRNA.
DR RefSeq; NP_001303376.1; NM_001316447.1.
DR RefSeq; NP_648150.1; NM_139893.4.
DR AlphaFoldDB; Q9VSA4; -.
DR SMR; Q9VSA4; -.
DR BioGRID; 64296; 3.
DR IntAct; Q9VSA4; 6.
DR STRING; 7227.FBpp0076526; -.
DR iPTMnet; Q9VSA4; -.
DR PaxDb; Q9VSA4; -.
DR EnsemblMetazoa; FBtr0076814; FBpp0076526; FBgn0035812.
DR EnsemblMetazoa; FBtr0346912; FBpp0312411; FBgn0035812.
DR GeneID; 38865; -.
DR KEGG; dme:Dmel_CG7457; -.
DR UCSC; CG7457-RA; d. melanogaster.
DR FlyBase; FBgn0035812; CG7457.
DR VEuPathDB; VectorBase:FBgn0035812; -.
DR eggNOG; KOG0504; Eukaryota.
DR HOGENOM; CLU_002128_0_0_1; -.
DR InParanoid; Q9VSA4; -.
DR OMA; AEICEQQ; -.
DR OrthoDB; 56880at2759; -.
DR PhylomeDB; Q9VSA4; -.
DR BioGRID-ORCS; 38865; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38865; -.
DR PRO; PR:Q9VSA4; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035812; Expressed in oocyte and 37 other tissues.
DR ExpressionAtlas; Q9VSA4; baseline and differential.
DR Genevisible; Q9VSA4; DM.
DR GO; GO:0043596; C:nuclear replication fork; ISS:FlyBase.
DR GO; GO:0042393; F:histone binding; ISS:FlyBase.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; ISS:FlyBase.
DR GO; GO:0031297; P:replication fork processing; ISS:FlyBase.
DR Gene3D; 1.25.40.10; -; 3.
DR Gene3D; 1.25.40.20; -; 1.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF13516; LRR_6; 2.
DR Pfam; PF13181; TPR_8; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 3.
DR SMART; SM00028; TPR; 6.
DR SUPFAM; SSF48403; SSF48403; 1.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 3.
DR PROSITE; PS50005; TPR; 6.
DR PROSITE; PS50293; TPR_REGION; 3.
PE 1: Evidence at protein level;
KW ANK repeat; Chromatin regulator; Chromosome; DNA damage; DNA repair;
KW Leucine-rich repeat; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW TPR repeat.
FT CHAIN 1..1405
FT /note="Tonsoku-like protein"
FT /id="PRO_0000403776"
FT REPEAT 23..56
FT /note="TPR 1"
FT REPEAT 63..96
FT /note="TPR 2"
FT REPEAT 153..181
FT /note="LRR 1"
FT REPEAT 163..196
FT /note="TPR 3"
FT REPEAT 203..237
FT /note="TPR 4"
FT REPEAT 245..278
FT /note="TPR 5"
FT REPEAT 314..347
FT /note="TPR 6"
FT REPEAT 355..388
FT /note="TPR 7"
FT REPEAT 439..465
FT /note="LRR 2"
FT REPEAT 538..567
FT /note="ANK 1"
FT REPEAT 571..600
FT /note="ANK 2"
FT REPEAT 609..638
FT /note="ANK 3"
FT REPEAT 1085..1108
FT /note="LRR 3"
FT REPEAT 1113..1137
FT /note="LRR 4"
FT REPEAT 1143..1166
FT /note="LRR 5"
FT REPEAT 1186..1211
FT /note="LRR 6"
FT REPEAT 1287..1311
FT /note="LRR 7"
FT REPEAT 1333..1357
FT /note="LRR 8"
FT REGION 465..535
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 695..753
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 806..827
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 841..880
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 728..751
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 707
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 709
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 893
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 895
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 899
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 902
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 535
FT /note="K -> R (in Ref. 3; AAM48411)"
FT /evidence="ECO:0000305"
FT CONFLICT 983
FT /note="R -> H (in Ref. 3; AAM48411)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1405 AA; 158302 MW; AD1062A025A82999 CRC64;
MEEKRYLKRK EKARSDGNRD QVAVSCNQLG DFYNQQGKYT DAVREYVQEA QIYASMGKEL
ETAKAKRMVG EMYTLLCDYD AAKDHINDYL KIAKRLKNQV EEQRAYATLG RVHLLHGQSL
ADSSASGSME QLKLAEKNFL RSLLLIKDLS GQISKLEQLD MQARCYLNIG VVKEHMEAFQ
ESIEYIDKAI KISKTHELWD LTHLCYISMS LLYICKKNDA TAALRFCNMA LEVAKRFPNK
VKKICETLIT KAEILIKAGD FASAKQILTK AYKKNTPDEN DRVNIEKQLR IVVKVCQTLD
ELVLTSSVDY AKLKGLYEKL GDGCCHLMNY EKALTYYQKM LENAELNQES GKSLVPIYVS
LYQTYRDNGQ FDKALEYLWK EFELNQDAPS EAFTTLCTIA EICEQQSHPF WTVHDVYQKA
LRQADKAGSC SDKLVKIAMV RLRRLMLKHN MQVLVENLEA DATAKGIDLD QEESVGDDEE
ESDGGGTAVQ QNTPDWDDDF DLATLTDSDA SDLDETEKPR PQRTTRGNRT LVIKKNNKGE
TQLHQACISG NLELVRRLID QGHTVNVRDH AGWLPLHEAC NHGYREIVEL LLDKGAASAI
NDKGGTSCDG ITPLFDACSN GFLDVAELLL DRGADATVRT DYNETCIAGL DKWRQGAQLV
DGEQAQYAQL RERLLRTLSK VGICSDKNAR PLTNFNAKRI SREERGSMSE EEDEEEALHE
SNRRSLSHNR SGSEYGAKKS KSSTQPSASK EYRSVMAHLK RPNRLNDDPP STSTLNKHKR
NAFLSEDEVD ADNWLIDDVG PERKRKRINS GDLSRRTSKE NFQDTALSLP ANWEDDLLQA
TPENEYSQRQ KQMRKLTLSR SSSMSSNHSS SATSSRKKHQ ATLLDSGFSR FRSESPLGSE
SSQDGTTSLI SVRTIEPDST TSTIQVLISP AKSSPIKVQA TPVLATTVSF KVKIQDELLL
VPIERKKLQD INIRWLAEEA GRRYNKLTGL TPLLRLKTAD GFAYEETDPV SVALEQNMLM
ASILDWKISP LSQRYEEMCL QMQKTVDNKV KLLLERSQNN NMLELSGLWM RAEKTEPIFK
ALLHQARLTV LDLSCNFIGN EGCQQLAKSL PTLLQLKALR LQCNAIGSHG LEALLCGQGM
DKLELLEELN LNQNPLGNAS VRILSKYCAS PAGQALTCLQ LAQCELTELQ DFDLGFNKLT
RFDISFNQLT QQSVRRLTDQ LNSCRLEQLN LSYVRWPLDD ASGFALSERL VTLFEGGTCE
RFVGVQLAGC GLNDAHMYNI SQHLAKAKQL QMLDISDNSN LSGTTLGYIL DELPQLRDLL
AVNCTNLLDD IRLQKLEQLK QLPRRLELTV DEQVFSMPGA LETLQSIWQL QFGDKAKMLT
TSNSRKIGRR YKGLLKLLAD GSDAE
