TONS_ARATH
ID TONS_ARATH Reviewed; 1311 AA.
AC Q6Q4D0; Q6IE93; Q769D5; Q7XAG5; Q9LSA4;
DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Protein TONSOKU {ECO:0000303|PubMed:15082530};
DE AltName: Full=Protein BRUSHY 1 {ECO:0000303|PubMed:15964904};
DE AltName: Full=Protein MGOUN 3 {ECO:0000303|PubMed:14966212};
GN Name=TSK {ECO:0000303|PubMed:15082530};
GN Synonyms=BRU1 {ECO:0000303|PubMed:15964904},
GN MGOUN3 {ECO:0000303|PubMed:14966212}; OrderedLocusNames=At3g18730;
GN ORFNames=MVE11.9;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS
RP OF GLY-190.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=15082530; DOI=10.1101/gad.295404;
RA Takeda S., Tadele Z., Hofmann I., Probst A.V., Angelis K.J., Kaya H.,
RA Araki T., Mengiste T., Scheid O.M., Shibahara K., Scheel D., Paszkowski J.;
RT "BRU1, a novel link between responses to DNA damage and epigenetic gene
RT silencing in Arabidopsis.";
RL Genes Dev. 18:782-793(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=15125773; DOI=10.1111/j.1365-313x.2004.02074.x;
RA Suzuki T., Inagaki S., Nakajima S., Akashi T., Ohto M.-A., Kobayashi M.,
RA Seki M., Shinozaki K., Kato T., Tabata S., Nakamura K., Morikami A.;
RT "A novel Arabidopsis gene TONSOKU is required for proper cell arrangement
RT in root and shoot apical meristems.";
RL Plant J. 38:673-684(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-1303, IDENTIFICATION, FUNCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia, and cv. Wassilewskija;
RX PubMed=14966212; DOI=10.1093/jxb/erh069;
RA Guyomarc'h S., Vernoux T., Traas J., Zhou D.-X., Delarue M.;
RT "MGOUN3, an Arabidopsis gene with tetratricopeptide-repeat-related motifs,
RT regulates meristem cellular organization.";
RL J. Exp. Bot. 55:673-684(2004).
RN [6]
RP INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15746155; DOI=10.1093/pcp/pci082;
RA Suzuki T., Nakajima S., Inagaki S., Hirano-Nakakita M., Matsuoka K.,
RA Demura T., Fukuda H., Morikami A., Nakamura K.;
RT "TONSOKU is expressed in S phase of the cell cycle and its defect delays
RT cell cycle progression in Arabidopsis.";
RL Plant Cell Physiol. 46:736-742(2005).
RN [7]
RP FUNCTION, INTERACTION WITH TSA1, TISSUE SPECIFICITY, AND SUBCELLULAR
RP LOCATION.
RX PubMed=15964904; DOI=10.1093/pcp/pci155;
RA Suzuki T., Nakajima S., Morikami A., Nakamura K.;
RT "An Arabidopsis protein with a novel calcium-binding repeat sequence
RT interacts with TONSOKU/MGOUN3/BRUSHY1 involved in meristem maintenance.";
RL Plant Cell Physiol. 46:1452-1461(2005).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=16728410; DOI=10.1093/jxb/erj169;
RA Guyomarc'h S., Benhamed M., Lemonnier G., Renou J.P., Zhou D.X.,
RA Delarue M.;
RT "MGOUN3: evidence for chromatin-mediated regulation of FLC expression.";
RL J. Exp. Bot. 57:2111-2119(2006).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=21705754; DOI=10.1534/genetics.111.130062;
RA Ohno Y., Narangajavana J., Yamamoto A., Hattori T., Kagaya Y.,
RA Paszkowski J., Gruissem W., Hennig L., Takeda S.;
RT "Ectopic gene expression and organogenesis in Arabidopsis mutants missing
RT BRU1 required for genome maintenance.";
RL Genetics 189:83-95(2011).
RN [10]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=35298257; DOI=10.1126/science.abm5320;
RA Davarinejad H., Huang Y.C., Mermaz B., LeBlanc C., Poulet A., Thomson G.,
RA Joly V., Munoz M., Arvanitis-Vigneault A., Valsakumar D., Villarino G.,
RA Ross A., Rotstein B.H., Alarcon E.I., Brunzelle J.S., Voigt P., Dong J.,
RA Couture J.F., Jacob Y.;
RT "The histone H3.1 variant regulates TONSOKU-mediated DNA repair during
RT replication.";
RL Science 375:1281-1286(2022).
CC -!- FUNCTION: Histone reader involved in homologous recombination-mediated
CC repair of double-strand breaks (DSBs) at stalled or collapsed
CC replication forks (PubMed:35298257). Specifically recognizes and binds
CC histone H3.1 lacking methylation at 'Lys-27' (H3K27me1)
CC (PubMed:35298257). Involved in structural and functional stabilization
CC of chromatin; represents a link between response to DNA damage and
CC epigenetic gene silencing (PubMed:14966212, PubMed:15082530,
CC PubMed:16728410, PubMed:21705754). Required for cell arrangement in
CC root and shoot apical meristems (PubMed:14966212, PubMed:15082530,
CC PubMed:16728410, PubMed:21705754). May be involved, when interacting
CC with TSA1, in the organization of spindle microtubules
CC (PubMed:15964904). {ECO:0000269|PubMed:14966212,
CC ECO:0000269|PubMed:15082530, ECO:0000269|PubMed:15964904,
CC ECO:0000269|PubMed:16728410, ECO:0000269|PubMed:21705754,
CC ECO:0000269|PubMed:35298257}.
CC -!- SUBUNIT: Interacts (via TPR repeats) with TSA1 (via C-terminal domain).
CC {ECO:0000269|PubMed:15964904}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm
CC {ECO:0000269|PubMed:15082530, ECO:0000269|PubMed:15125773,
CC ECO:0000269|PubMed:15746155, ECO:0000269|PubMed:15964904,
CC ECO:0000269|PubMed:21705754}. Chromosome {ECO:0000269|PubMed:35298257}.
CC Note=During anaphase, relocalizes in spots close to the ends of spindle
CC microtubules ahead of separating chromatids.
CC -!- TISSUE SPECIFICITY: Preferentially expressed in the shoot apex than in
CC the leaves and stems. Expressed in the shoot apex and root tips in 5-
CC day-old seedlings. At the shoot apex it is expressed in young leaves
CC and in broad areas between two cotyledons. Weakly or not expressed in
CC vascular tissues except vascular tissue near the shoot apical meristem.
CC In the root, it extends from the root apical meristem to the elongation
CC zone. {ECO:0000269|PubMed:15125773, ECO:0000269|PubMed:15964904}.
CC -!- INDUCTION: Under cell cycle-dependent control with a peak of expression
CC at the S phase. {ECO:0000269|PubMed:15746155}.
CC -!- DISRUPTION PHENOTYPE: Abnormal enlargement of the shoot apical meristem
CC associated with a disrupted cellular and subcellular organization.
CC Release of transcriptional gene silencing of heterochromatic genes, and
CC ectopic gene expression and organogenesis. Under-expression of FLC and
CC early flowering in short days. {ECO:0000269|PubMed:14966212,
CC ECO:0000269|PubMed:16728410, ECO:0000269|PubMed:21705754}.
CC -!- SIMILARITY: Belongs to the Tonsoku family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB01799.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY560347; AAS67383.1; -; mRNA.
DR EMBL; AB112744; BAD04041.1; -; mRNA.
DR EMBL; AB026654; BAB01799.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE76137.1; -; Genomic_DNA.
DR EMBL; AJ579629; CAE22479.1; -; mRNA.
DR EMBL; BN000267; CAE30337.1; -; mRNA.
DR RefSeq; NP_188503.2; NM_112759.5.
DR AlphaFoldDB; Q6Q4D0; -.
DR SMR; Q6Q4D0; -.
DR BioGRID; 6737; 3.
DR STRING; 3702.AT3G18730.1; -.
DR iPTMnet; Q6Q4D0; -.
DR PaxDb; Q6Q4D0; -.
DR PRIDE; Q6Q4D0; -.
DR ProteomicsDB; 234337; -.
DR EnsemblPlants; AT3G18730.1; AT3G18730.1; AT3G18730.
DR GeneID; 821404; -.
DR Gramene; AT3G18730.1; AT3G18730.1; AT3G18730.
DR KEGG; ath:AT3G18730; -.
DR Araport; AT3G18730; -.
DR TAIR; locus:2094019; AT3G18730.
DR eggNOG; KOG0619; Eukaryota.
DR eggNOG; KOG1124; Eukaryota.
DR HOGENOM; CLU_006349_0_0_1; -.
DR InParanoid; Q6Q4D0; -.
DR OMA; VKPCCRK; -.
DR OrthoDB; 52803at2759; -.
DR PhylomeDB; Q6Q4D0; -.
DR PRO; PR:Q6Q4D0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q6Q4D0; baseline and differential.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0042393; F:histone binding; IDA:UniProtKB.
DR GO; GO:0051301; P:cell division; IMP:TAIR.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IDA:UniProtKB.
DR GO; GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:TAIR.
DR GO; GO:0009933; P:meristem structural organization; IMP:TAIR.
DR GO; GO:0040029; P:regulation of gene expression, epigenetic; IEA:InterPro.
DR GO; GO:0009934; P:regulation of meristem structural organization; IMP:TAIR.
DR GO; GO:0031297; P:replication fork processing; IDA:UniProtKB.
DR GO; GO:0072423; P:response to DNA damage checkpoint signaling; IEA:InterPro.
DR Gene3D; 1.25.40.10; -; 3.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR044227; TONSOKU.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR47684; PTHR47684; 1.
DR Pfam; PF13516; LRR_6; 1.
DR Pfam; PF13181; TPR_8; 1.
DR SMART; SM00028; TPR; 6.
DR SUPFAM; SSF48452; SSF48452; 3.
PE 1: Evidence at protein level;
KW Chromatin regulator; Chromosome; Coiled coil; DNA damage; DNA repair;
KW Leucine-rich repeat; Nucleus; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..1311
FT /note="Protein TONSOKU"
FT /id="PRO_0000106266"
FT REPEAT 26..59
FT /note="TPR 1"
FT REPEAT 66..99
FT /note="TPR 2"
FT REPEAT 106..147
FT /note="TPR 3"
FT REPEAT 164..197
FT /note="TPR 4"
FT REPEAT 207..240
FT /note="TPR 5"
FT REPEAT 247..280
FT /note="TPR 6"
FT REPEAT 378..411
FT /note="TPR 7"
FT REPEAT 418..451
FT /note="TPR 8"
FT REPEAT 810..832
FT /note="LRR 1"
FT REPEAT 839..860
FT /note="LRR 2"
FT REPEAT 866..886
FT /note="LRR 3"
FT REPEAT 898..918
FT /note="LRR 4"
FT REPEAT 925..946
FT /note="LRR 5"
FT REPEAT 953..976
FT /note="LRR 6"
FT REPEAT 981..1002
FT /note="LRR 7"
FT REGION 492..533
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 297..333
FT /evidence="ECO:0000255"
FT COILED 475..504
FT /evidence="ECO:0000255"
FT COMPBIAS 492..514
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..533
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 190
FT /note="G->R: In bru1-2; induces growth retardation of
FT primary roots, distorded phyllotaxy and fasciation."
FT /evidence="ECO:0000269|PubMed:15082530"
FT CONFLICT 641
FT /note="N -> S (in Ref. 5; CAE22479/CAE30337)"
FT /evidence="ECO:0000305"
FT CONFLICT 780
FT /note="V -> G (in Ref. 1; AAS67383)"
FT /evidence="ECO:0000305"
FT CONFLICT 920
FT /note="P -> R (in Ref. 1; AAS67383)"
FT /evidence="ECO:0000305"
FT CONFLICT 1130
FT /note="S -> N (in Ref. 1; AAS67383)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1311 AA; 146482 MW; D1F7C3D63CC9187C CRC64;
MGRLDVAAAK RAYRKAEEVG DRREQARWAN NVGDILKNHG EYVDALKWFR IDYDISVKYL
PGKDLLPTCQ SLGEIYLRLE NFEEALIYQK KHLQLAEEAN DTVEKQRACT QLGRTYHEMF
LKSEDDCEAI QSAKKYFKKA MELAQILKEK PPPGESSGFL EEYINAHNNI GMLDLDLDNP
EAARTILKKG LQICDEEEVR EYDAARSRLH HNLGNVFMAL RSWDEAKKHI EMDINICHKI
NHVQGEAKGY INLAELHNKT QKYIDALLCY GKASSLAKSM QDESALVEQI EHNTKIVKKS
MKVMEELREE ELMLKKLSAE MTDAKGTSEE RKSMLQVNAC LGSLIDKSSM VFAWLKHLQY
SKRKKKISDE LCDKEKLSDA FMIVGESYQN LRNFRKSLKW FIRSYEGHEA IGNLEGQALA
KINIGNGLDC IGEWTGALQA YEEGYRIALK ANLPSIQLSA LEDIHYIHMM RFGNAQKASE
LKETIQNLKE SEHAEKAECS TQDECSETDS EGHANVSNDR PNACSSPQTP NSLRSERLAD
LDEANDDVPL ISFLQPGKRL FKRKQVSGKQ DADTDQTKKD FSVVADSQQT VAGRKRIRVI
LSDDESETEY ELGCPKDSSH KVLRQNEEVS EESMYFDGAI NYTDNRAIQD NVEEGSCSYT
PLHPIKVAPN VSNCRSLSNN IAVETTGRRK KGSQCDVGDS NGTSCKTGAA LVNFHAYSKT
EDRKIKIEIE NEHIALDSCS HDDESVKVEL TCLYYLQLPD DEKSKGLLPI IHHLEYGGRV
LKPLELYAIL RDSSENVVIE ASVDGWVHKR LMKLYMDCCQ SLSEKPSMKL LKKLYISEVE
DDINVSECEL QDISAAPLLC ALHVHNIAML DLSHNMLGNG TMEKLKQLFA SSSQMYGALT
LDLHCNRFGP TALFQICECP VLFTRLEVLN VSRNRLTDAC GSYLSTIVKN CRALYSLNVE
HCSLTSRTIQ KVANALDSKS GLSQLCIGYN NPVSGSSIQN LLAKLATLSS FAELSMNGIK
LSSQVVDSLY ALVKTPSLSK LLVGSSGIGT DGAIKVTESL CYQKEETVKL DLSCCGLASS
FFIKLNQDVT LTSSILEFNV GGNPITEEGI SALGELLRNP CSNIKVLILS KCHLKLAGLL
CIIQALSDNK NLEELNLSDN AKIEDETVFG QPVKERSVMV EQEHGTCKSV TSMDKEQELC
ETNMECDDLE VADSEDEQIE EGTATSSSLS LPRKNHIVKE LSTALSMANQ LKILDLSNNG
FSVEALETLY MSWSSSSSRT GIAQRHVKEE TVHFYVEGKM CCGVKSCCRK D
