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TOP1M_RAT
ID   TOP1M_RAT               Reviewed;         593 AA.
AC   Q6IM78;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 121.
DE   RecName: Full=DNA topoisomerase I, mitochondrial;
DE            Short=TOP1mt;
DE            EC=5.6.2.1;
DE   Flags: Precursor;
GN   Name=Top1mt;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=15096574; DOI=10.1093/nar/gkh525;
RA   Zhang H., Meng L.-H., Zimonjic D.B., Popescu N.C., Pommier Y.;
RT   "Thirteen-exon-motif signature for vertebrate nuclear and mitochondrial
RT   type IB topoisomerases.";
RL   Nucleic Acids Res. 32:2087-2092(2004).
CC   -!- FUNCTION: Releases the supercoiling and torsional tension of DNA
CC       introduced during duplication of mitochondrial DNA by transiently
CC       cleaving and rejoining one strand of the DNA duplex. Introduces a
CC       single-strand break via transesterification at a target site in duplex
CC       DNA. The scissile phosphodiester is attacked by the catalytic tyrosine
CC       of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-
CC       enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free
CC       DNA strand then rotates around the intact phosphodiester bond on the
CC       opposing strand, thus removing DNA supercoils. Finally, in the
CC       religation step, the DNA 5'-OH attacks the covalent intermediate to
CC       expel the active-site tyrosine and restore the DNA phosphodiester
CC       backbone (By similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC         passage and rejoining.; EC=5.6.2.1;
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Evidence={ECO:0000250};
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC       Note=Divalent metal ions (calcium or magnesium). {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the type IB topoisomerase family. {ECO:0000305}.
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DR   EMBL; BK001786; DAA02296.1; -; mRNA.
DR   RefSeq; NP_001002798.1; NM_001002798.1.
DR   AlphaFoldDB; Q6IM78; -.
DR   SMR; Q6IM78; -.
DR   STRING; 10116.ENSRNOP00000009965; -.
DR   PaxDb; Q6IM78; -.
DR   Ensembl; ENSRNOT00000009965; ENSRNOP00000009965; ENSRNOG00000007500.
DR   GeneID; 300029; -.
DR   KEGG; rno:300029; -.
DR   UCSC; RGD:1303177; rat.
DR   CTD; 116447; -.
DR   RGD; 1303177; Top1mt.
DR   eggNOG; KOG0981; Eukaryota.
DR   GeneTree; ENSGT00940000162943; -.
DR   InParanoid; Q6IM78; -.
DR   OMA; RNYIDPR; -.
DR   OrthoDB; 303947at2759; -.
DR   PhylomeDB; Q6IM78; -.
DR   TreeFam; TF105281; -.
DR   PRO; PR:Q6IM78; -.
DR   Proteomes; UP000002494; Chromosome 7.
DR   Bgee; ENSRNOG00000007500; Expressed in testis and 19 other tissues.
DR   ExpressionAtlas; Q6IM78; baseline and differential.
DR   Genevisible; Q6IM78; RN.
DR   GO; GO:0005694; C:chromosome; IEA:InterPro.
DR   GO; GO:0042645; C:mitochondrial nucleoid; ISO:RGD.
DR   GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IBA:GO_Central.
DR   GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR   GO; GO:0006265; P:DNA topological change; IBA:GO_Central.
DR   CDD; cd00659; Topo_IB_C; 1.
DR   Gene3D; 1.10.10.41; -; 1.
DR   Gene3D; 1.10.132.10; -; 1.
DR   Gene3D; 2.170.11.10; -; 1.
DR   Gene3D; 3.90.15.10; -; 1.
DR   InterPro; IPR011010; DNA_brk_join_enz.
DR   InterPro; IPR013034; DNA_topo_DNA_db_N_dom1.
DR   InterPro; IPR013030; DNA_topo_DNA_db_N_dom2.
DR   InterPro; IPR001631; TopoI.
DR   InterPro; IPR025834; TopoI_C_dom.
DR   InterPro; IPR014711; TopoI_cat_a-hlx-sub_euk.
DR   InterPro; IPR014727; TopoI_cat_a/b-sub_euk.
DR   InterPro; IPR013500; TopoI_cat_euk.
DR   InterPro; IPR008336; TopoI_DNA-bd_euk.
DR   InterPro; IPR036202; TopoI_DNA-bd_euk_N_sf.
DR   InterPro; IPR013499; TopoI_euk.
DR   Pfam; PF14370; Topo_C_assoc; 1.
DR   Pfam; PF01028; Topoisom_I; 1.
DR   Pfam; PF02919; Topoisom_I_N; 1.
DR   PRINTS; PR00416; EUTPISMRASEI.
DR   SMART; SM00435; TOPEUc; 1.
DR   SUPFAM; SSF56349; SSF56349; 1.
DR   SUPFAM; SSF56741; SSF56741; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Isomerase; Mitochondrion; Reference proteome; Topoisomerase;
KW   Transit peptide.
FT   TRANSIT         1..43
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           44..593
FT                   /note="DNA topoisomerase I, mitochondrial"
FT                   /id="PRO_0000384395"
FT   REGION          254..255
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   REGION          317..322
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   REGION          414..416
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        551
FT                   /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   SITE            145
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            193
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            241
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            272
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            330
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            361
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            403
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            461
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
FT   SITE            479
FT                   /note="Interaction with DNA"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   593 AA;  69011 MW;  6B691D668970BD9E CRC64;
     MLLLWLRALC RRFQHVPRRV PSRQVSRGSK ASRAGWGETS KSSVKWKQLE HKGPCFAPAY
     EPLPDGVRFF YDGKPVRLSL AAEEVATFYG KMLHLECTTK EVFRRNFFSD WQKEMTAEER
     KLITHLDKCD FSEIHRHFME RAEARRTLPR EQKQKLKEEA EKLQQEFGYC ILDGHREKIG
     NFKTEPPGLF RGRGDHPKMG MLKRRVMPED VVINCSRDSK IPEPPAGHQW KEVRSDNTVM
     WLAAWVENIQ NSFKYIILNP SSKPKGEMDW QKYEVARRLK GVVDKIRAQY QADWKSPEMK
     KRQLAVALYF IDKLALRTGN EKEEGETADT VGCCSLRVEH VRLHTPADGQ EHVVELDFLG
     KDSIRYKNHV TVEKLVFQNL QHFMEDKDPR DDLFDALTTS SLNKHLQDLM EGLTAKVFRT
     YNASITLQEQ LRVLTRAEDS LTCKVLAYNR ANRAVAVLCN HQRAIPKTFE ESMQTLQKKI
     ETKKAQVAEA QVELQKAETD LRMRGDSKSK SFLQKQQRLL KLEEQLARLC TKATDKEENK
     QVALGTAKLN YLDPRISIAW CKRFGVPVEK IYNKTQRERF AWAFNQAGED FEF
 
 
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