ID   BTRH_NIACI              Reviewed;         302 AA.
AC   Q4H4E9;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Ribostamycin:4-(gamma-L-glutamylamino)-(S)-2-hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(gamma-L-glutamylamino)-(S)-2-hydroxybutanoate transferase;
DE            EC=2.3.2.19;
DE   AltName: Full=Acyl carrier protein:aminoglycoside acyltransferase;
DE   AltName: Full=Butirosin biosynthesis protein H;
GN   Name=btrH;
OS   Niallia circulans (Bacillus circulans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Niallia.
OX   NCBI_TaxID=1397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 21557 / NCIMB 12336 / BU-1709-YQW-B6;
RX   PubMed=16156513; DOI=10.1038/ja.2005.47;
RA   Kudo F., Numakura M., Tamegai H., Yamamoto H., Eguchi T., Kakinuma K.;
RT   "Extended sequence and functional analysis of the butirosin biosynthetic
RT   gene cluster in Bacillus circulans SANK 72073.";
RL   J. Antibiot. 58:373-379(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 21557 / NCIMB 12336 / BU-1709-YQW-B6;
RA   Aboshanab K.M., Schmidt-Beissner H., Wehmeier U.F., Welzel K., Vente A.,
RA   Piepersberg W.;
RT   "Analysis and comparison of the biosynthetic gene clusters for the 2-
RT   deoxystreptamine-containing aminoglycoside antibiotics ribostamycin,
RT   neomycin, lividomycin, paromomycin and butirosin.";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC   STRAIN=ATCC 21557 / NCIMB 12336 / BU-1709-YQW-B6;
RX   PubMed=17462573; DOI=10.1016/j.chembiol.2007.02.005;
RA   Llewellyn N.M., Li Y., Spencer J.B.;
RT   "Biosynthesis of butirosin: transfer and deprotection of the unique amino
RT   acid side chain.";
RL   Chem. Biol. 14:379-386(2007).
CC   -!- FUNCTION: Aminoglycoside acyltransferase that attaches the (S)-4-amino-
CC       2-hydroxybutyrate (AHBA) side chain from the acyl carrier protein BtrI
CC       to the aminoglycoside ribostamycin in the biosynthetic pathway of
CC       butirosin. The AHBA side chain protects the antibiotic from several
CC       common resistance mechanisms. {ECO:0000269|PubMed:17462573}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-(gamma-L-glutamylamino)-(2S)-2-hydroxybutanoyl-[BtrI ACP] +
CC         ribostamycin = gamma-L-glutamyl-butirosin B + H(+) + holo-[BtrI ACP];
CC         Xref=Rhea:RHEA:53964, Rhea:RHEA-COMP:13741, Rhea:RHEA-COMP:13743,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:64479, ChEBI:CHEBI:65028,
CC         ChEBI:CHEBI:65086, ChEBI:CHEBI:137999; EC=2.3.2.19;
CC         Evidence={ECO:0000269|PubMed:17462573};
CC   -!- PATHWAY: Antibiotic biosynthesis; butirosin biosynthesis.
CC       {ECO:0000269|PubMed:17462573}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB097196; BAE07072.1; -; Genomic_DNA.
DR   EMBL; AJ781030; CAG77426.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q4H4E9; -.
DR   KEGG; ag:BAE07072; -.
DR   BioCyc; MetaCyc:MON-17275; -.
DR   UniPathway; UPA00964; -.
DR   GO; GO:0016755; F:aminoacyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IDA:UniProtKB.
DR   InterPro; IPR026935; BtrH_N.
DR   Pfam; PF14399; BtrH_N; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; Antibiotic biosynthesis; Transferase.
FT   CHAIN           1..302
FT                   /note="Ribostamycin:4-(gamma-L-glutamylamino)-(S)-2-
FT                   hydroxybutanoyl-[BtrI acyl-carrier protein] 4-(gamma-L-
FT                   glutamylamino)-(S)-2-hydroxybutanoate transferase"
FT                   /id="PRO_0000421730"
SQ   SEQUENCE   302 AA;  35885 MW;  795D0260A190B425 CRC64;
     MCLTRYDEKF FDCRKSQIIA YLDSQQVPVI PLFYNSYQST AEIYRQIFIE NKSKWKYSEP
     SFSDDDLLRK GIRPVRASFP DFSQASDCLK DLLARHKLVF VWGDEYCLPY RKEAFQAIHS
     THSLVVTGYD GENKAYYVED WDGLYGYLPA VHLEAAFDSL SRQMRTLLVL ELNDEEMREN
     KQEDTDLFRK WLQAFEDDYI FYDRVLLDMR DYEENRLISM DHGLRLIAAS RHVFSKFLHY
     IDDAPEEVGL LIRNHQLANH IAAIVRRYII AKQIDWDGAA CKIRQLREQE DDFMRKLKSR
     YG