ID   BTRL_NIACI              Reviewed;         604 AA.
AC   Q4H4F7;
DT   06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT   30-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Neamine phosphoribosyltransferase;
DE            EC=2.4.2.49;
DE   AltName: Full=Butirosin biosynthesis protein P;
GN   Name=btrL;
OS   Niallia circulans (Bacillus circulans).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Niallia.
OX   NCBI_TaxID=1397;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 21557 / NCIMB 12336 / BU-1709-YQW-B6;
RX   PubMed=16156513; DOI=10.1038/ja.2005.47;
RA   Kudo F., Numakura M., Tamegai H., Yamamoto H., Eguchi T., Kakinuma K.;
RT   "Extended sequence and functional analysis of the butirosin biosynthetic
RT   gene cluster in Bacillus circulans SANK 72073.";
RL   J. Antibiot. 58:373-379(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 21557 / NCIMB 12336 / BU-1709-YQW-B6;
RA   Aboshanab K.M., Schmidt-Beissner H., Wehmeier U.F., Welzel K., Vente A.,
RA   Piepersberg W.;
RT   "Analysis and comparison of the biosynthetic gene clusters for the 2-
RT   deoxystreptamine-containing aminoglycoside antibiotics ribostamycin,
RT   neomycin, lividomycin, paromomycin and butirosin.";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, AND
RP   PATHWAY.
RC   STRAIN=SANK 72073;
RX   PubMed=17482823; DOI=10.1016/j.bmc.2007.04.040;
RA   Kudo F., Fujii T., Kinoshita S., Eguchi T.;
RT   "Unique O-ribosylation in the biosynthesis of butirosin.";
RL   Bioorg. Med. Chem. 15:4360-4368(2007).
CC   -!- FUNCTION: Catalyzes phosphoribosylation of neamine using 5-
CC       phosphoribosyl-1-diphosphate (PRPP) as a phosphoribosyl donor to
CC       generate 5''-phosphoribostamycin in the biosynthetic pathway of
CC       butirosin. {ECO:0000269|PubMed:17482823}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-phospho-alpha-D-ribose 1-diphosphate + neamine = 5''-
CC         phosphoribostamycin + diphosphate; Xref=Rhea:RHEA:34043,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58017, ChEBI:CHEBI:65076,
CC         ChEBI:CHEBI:65082; EC=2.4.2.49;
CC         Evidence={ECO:0000269|PubMed:17482823};
CC   -!- COFACTOR:
CC       Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC         Evidence={ECO:0000269|PubMed:17482823};
CC       Note=Divalent metal ion. {ECO:0000269|PubMed:17482823};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.5. {ECO:0000269|PubMed:17482823};
CC       Temperature dependence:
CC         Optimum temperature is 27-32 degrees Celsius.
CC         {ECO:0000269|PubMed:17482823};
CC   -!- PATHWAY: Antibiotic biosynthesis; butirosin biosynthesis.
CC       {ECO:0000269|PubMed:17482823}.
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DR   EMBL; AB097196; BAE07064.1; -; Genomic_DNA.
DR   EMBL; AJ781030; CAG77418.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q4H4F7; -.
DR   KEGG; ag:BAE07064; -.
DR   BioCyc; MetaCyc:MON-17254; -.
DR   UniPathway; UPA00964; -.
DR   GO; GO:0016763; F:pentosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IDA:UniProtKB.
DR   InterPro; IPR029057; PRTase-like.
DR   SUPFAM; SSF53271; SSF53271; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..604
FT                   /note="Neamine phosphoribosyltransferase"
FT                   /id="PRO_0000421727"
SQ   SEQUENCE   604 AA;  68618 MW;  E848DDB3E02606EC CRC64;
     MKDNPISLFH ELESYVRGSK DTLHRYMYAC ILEQRLADLL LLSRCGVHPD DEDRLAVRLA
     SQRSLCVQAA SDLAEGTEEA RGSTPAWPDC KVTKSSAAQK RPELLSLVKV MQELRTPECS
     GYDLCDLEVC REDALHWLRD HADPSSPPVL IGVRTGGAFY APLWSSALQQ RWGNKALYHT
     VRALRMPSDP GASLYLPEEL SILPAAIEPD TDIVILEDQP HTGGTVLELA GRLSAKYRLN
     KPVWVSSPGR LFQIENGRLA KCSDRIPLNT GTRKRIWQML GNSEEVAEFL LPKLGLTDAH
     PADLEAVPYK SVPQWNDPMY RREVPFRINP KKTPFYIRRK STNEPVVFAK FIGKDLFGDF
     QFHQLKKFEK YFPDILAYQD GYVMTKYEPG LKEMREMFVN MPSAVTREIC QSVSGYWKTL
     LDSCQISGGH PVHPLADHWQ ARLGEMEDFI GRKLPYDLDW FERSLHTEWT SSAHVYTSLP
     YANQYGHWKA RLSAGQRLKT YRFHIDSTWG GTSSIEVELA SFLLENRVRP DDFRLLVNQV
     KKEAGSLTIE AVTDALPIAC VLQAANLLKQ AKDESRLSKE LILSEAMQSF EYLQAMKPKL
     SDVR