TOP6A_ORYSI
ID TOP6A_ORYSI Reviewed; 442 AA.
AC A2XFC1; Q5ZPV7;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 03-APR-2013, sequence version 2.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=DNA topoisomerase 6 subunit A3 {ECO:0000255|HAMAP-Rule:MF_03164};
DE Short=OsTOP6A3;
DE EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_03164};
DE AltName: Full=DNA topoisomerase 6 subunit A {ECO:0000255|HAMAP-Rule:MF_03164};
DE Short=OsTOP6A;
DE AltName: Full=Meiotic recombination protein SPO11-3;
GN Name=TOP6A3 {ECO:0000255|HAMAP-Rule:MF_03164}; Synonyms=SPO11-3, TOP6A;
GN ORFNames=OsI_11065;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TOP6B, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Pusa Basmati;
RX PubMed=17116242; DOI=10.1111/j.1742-4658.2006.05518.x;
RA Jain M., Tyagi A.K., Khurana J.P.;
RT "Overexpression of putative topoisomerase 6 genes from rice confers stress
RT tolerance in transgenic Arabidopsis plants.";
RL FEBS J. 273:5245-5260(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Component of the DNA topoisomerase VI involved in chromatin
CC organization and progression of endoreduplication cycles. Relaxes both
CC positive and negative superturns and exhibits a strong decatenase
CC activity (By similarity). May be involved in cell proliferation and
CC stress tolerance. {ECO:0000255|HAMAP-Rule:MF_03164}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_03164};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_03164};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two TOP6A and two TOP6B subunits
CC (By similarity). Interacts with TOP6B. {ECO:0000255|HAMAP-
CC Rule:MF_03164, ECO:0000269|PubMed:17116242}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03164,
CC ECO:0000269|PubMed:17116242}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers before pollination.
CC Expressed in roots and shoots. {ECO:0000269|PubMed:17116242}.
CC -!- INDUCTION: By auxin, abscisic acid (ABA) and benzylaminopurine.
CC {ECO:0000269|PubMed:17116242}.
CC -!- SIMILARITY: Belongs to the TOP6A family. {ECO:0000255|HAMAP-
CC Rule:MF_03164}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAY89531.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ550618; CAD79468.1; -; mRNA.
DR EMBL; CM000128; EAY89531.1; ALT_SEQ; Genomic_DNA.
DR AlphaFoldDB; A2XFC1; -.
DR SMR; A2XFC1; -.
DR STRING; 39946.A2XFC1; -.
DR HOGENOM; CLU_354665_0_0_1; -.
DR Proteomes; UP000007015; Chromosome 3.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0009330; C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
DR CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR HAMAP; MF_00132; Top6A; 1.
DR InterPro; IPR002815; Spo11/TopoVI_A.
DR InterPro; IPR013049; Spo11/TopoVI_A_N.
DR InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR InterPro; IPR004085; TopoVI_A.
DR InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR10848; PTHR10848; 1.
DR Pfam; PF04406; TP6A_N; 1.
DR PRINTS; PR01550; TOP6AFAMILY.
DR PRINTS; PR01552; TPISMRASE6A.
DR SUPFAM; SSF56726; SSF56726; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Isomerase; Magnesium; Metal-binding; Nucleotide-binding;
KW Nucleus; Reference proteome; Topoisomerase.
FT CHAIN 1..442
FT /note="DNA topoisomerase 6 subunit A3"
FT /id="PRO_0000421934"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 185
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03164"
FT BINDING 271
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03164"
FT BINDING 323
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03164"
FT CONFLICT 99
FT /note="R -> S (in Ref. 1; CAD79468)"
FT /evidence="ECO:0000305"
FT CONFLICT 136
FT /note="D -> N (in Ref. 1; CAD79468)"
FT /evidence="ECO:0000305"
FT CONFLICT 166
FT /note="S -> F (in Ref. 1; CAD79468)"
FT /evidence="ECO:0000305"
FT CONFLICT 357
FT /note="L -> P (in Ref. 1; CAD79468)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 442 AA; 48242 MW; 1397867773A726BB CRC64;
MSEKKRRGGA GAGAASGSAS KKPRVSTAAS YAESLRSKLR PDASILATLR SLASACSKSK
PAGSSSSSSS ASKALAAEDD PAASYIVVAD QDSASVTSRI NRLVLAAARS ILSGRGFSFA
VPSRAASNQV YLPDLDRIVL VRRESARPFA NVATARKATI TARVLSLVHA VLRRGIHVTK
RDLFYTDVKL FGDQAQSDAV LDDVSCMLGC TRSSLHVVAS EKGVVVGRLT FADDGDRIDC
TRMGVGGKAI PPNIDRVSGI ESDALFILLV EKDAAFMRLA EDRFYNRFPC IILTAKGQPD
VATRLFLRRL KVELKLPVLA LVDSDPYGLK ILSVYMCGSK NMSYDSANLT TPDIKWLGVR
PSDLDKYRVP EQCRLPMTDH DIKVGKELLE EDFVKQNEGW VKELETMLRT RQKAEIQALS
SFGFQYLTEV YLPLKLQQQD WI
