TOP6B_NATPD
ID TOP6B_NATPD Reviewed; 798 AA.
AC Q3IPW6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Type 2 DNA topoisomerase 6 subunit B {ECO:0000255|HAMAP-Rule:MF_00322};
DE EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_00322};
DE AltName: Full=Type II DNA topoisomerase VI subunit B {ECO:0000255|HAMAP-Rule:MF_00322};
DE Short=TopoVI-B {ECO:0000255|HAMAP-Rule:MF_00322};
GN Name=top6B {ECO:0000255|HAMAP-Rule:MF_00322}; OrderedLocusNames=NP_3482A;
OS Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM
OS 8858 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Haloarculaceae; Natronomonas.
OX NCBI_TaxID=348780;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB
RC 2260 / Gabara;
RX PubMed=16169924; DOI=10.1101/gr.3952905;
RA Falb M., Pfeiffer F., Palm P., Rodewald K., Hickmann V., Tittor J.,
RA Oesterhelt D.;
RT "Living with two extremes: conclusions from the genome sequence of
RT Natronomonas pharaonis.";
RL Genome Res. 15:1336-1343(2005).
CC -!- FUNCTION: Relaxes both positive and negative superturns and exhibits a
CC strong decatenase activity. {ECO:0000255|HAMAP-Rule:MF_00322}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00322};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two Top6A and two Top6B chains.
CC {ECO:0000255|HAMAP-Rule:MF_00322}.
CC -!- SIMILARITY: Belongs to the TOP6B family. {ECO:0000255|HAMAP-
CC Rule:MF_00322}.
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DR EMBL; CR936257; CAI49832.1; -; Genomic_DNA.
DR RefSeq; WP_011323452.1; NC_007426.1.
DR AlphaFoldDB; Q3IPW6; -.
DR SMR; Q3IPW6; -.
DR STRING; 348780.NP_3482A; -.
DR EnsemblBacteria; CAI49832; CAI49832; NP_3482A.
DR GeneID; 3703276; -.
DR KEGG; nph:NP_3482A; -.
DR eggNOG; arCOG01165; Archaea.
DR HOGENOM; CLU_006403_0_0_2; -.
DR OMA; TRIELEM; -.
DR OrthoDB; 13565at2157; -.
DR Proteomes; UP000002698; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd00823; TopoIIB_Trans; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR HAMAP; MF_00322; Top6B; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR040494; Top6b_C.
DR InterPro; IPR005734; TopoVI_B.
DR InterPro; IPR015320; TopoVI_B_transducer.
DR PANTHER; PTHR10871:SF4; PTHR10871:SF4; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF18000; Top6b_C; 1.
DR Pfam; PF09239; Topo-VIb_trans; 1.
DR SMART; SM00387; HATPase_c; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR TIGRFAMs; TIGR01052; top6b; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA-binding; Isomerase; Nucleotide-binding;
KW Reference proteome; Topoisomerase.
FT CHAIN 1..798
FT /note="Type 2 DNA topoisomerase 6 subunit B"
FT /id="PRO_1000005872"
FT REGION 221..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 60
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00322"
FT BINDING 91
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00322"
FT BINDING 112..113
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00322"
FT BINDING 122..129
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00322"
FT BINDING 629
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00322"
SQ SEQUENCE 798 AA; 87363 MW; 82DF9847312DB62A CRC64;
MTSYQSQLGD GGGGEPIAEE LAESQQSISI AEFFEKNKQM LGFDSDAKAL VTAVKEAVDN
ALDAAEEAGI LPDIYVEIED RGDYYRLTVE DNGPGITKEQ IPKVFGKLLY GSRFHAREQT
RGQQGIGISA AVLYSQLTSG KPAKITSKTD SHSSAQYFEL TIDTDTNEPE IDHEREATWE
RPHGTRIELE MEGNMRARKQ LHDYIKYTAV VNPHARVEFH EPEDSFKSER ATEELPPETE
EIRPHPHGVE LGTLLKMLDA TDSYSLSGFL QAEFTRVGNK TADGVIDSFR DRHFGREMAW
SPPQPHEDVD IETAVSDAVA NKSAEATAAF ARAVADEVTD MAALSHAELS AVVASVADDI
EAEHDETFGE TVRENAVEAA WKSVTDDVSA DCYALVDGAT TTRKDDAAVE GLSRRLAEKF
TDQEDARNRF RRSTLREFVD RAADATEEYD DATFGETARE NVVEAVWERA VTVPDEPPTV
TEVADNRDAA ARLLEAMRST DILSPPTDCL APISEDLVKA GLKKEYDAEF YTSVTRDASV
HGGDPFVVEV GIAHGGDIAV DGSVETLRFA NRVPLVYQRG ACATVDVLKG VNWRNYGLDQ
PGGSGMPSGP AVIMVHVAST SVPFTSESKD AVANIPEIED ELRLALQQAG RDLQSHLKKQ
RSLEKRRRKQ DVIADILPDM AAKLSEVTDR EPLDVEDSLA RIMNNVLVER TVEESTVQLS
VHNYGDTNVG PEVTDIVSQE PDGAESATVV EMDGEWFLKW SPTVGGGETA TLEYEVDGDA
EFDISVEGIE AEKLTVDA
