TOP6B_ORYSI
ID TOP6B_ORYSI Reviewed; 696 AA.
AC B8BDQ4; Q5ZPR4;
DT 03-APR-2013, integrated into UniProtKB/Swiss-Prot.
DT 03-MAR-2009, sequence version 1.
DT 03-AUG-2022, entry version 73.
DE RecName: Full=DNA topoisomerase 6 subunit B {ECO:0000255|HAMAP-Rule:MF_03165};
DE Short=OsTOP6B;
DE EC=5.6.2.2 {ECO:0000255|HAMAP-Rule:MF_03165};
GN Name=TOP6B {ECO:0000255|HAMAP-Rule:MF_03165}; ORFNames=OsI_30680;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP INDUCTION, AND INTERACTION WITH SPO11-2 AND TOP6A3.
RC STRAIN=cv. Pusa Basmati;
RX PubMed=17116242; DOI=10.1111/j.1742-4658.2006.05518.x;
RA Jain M., Tyagi A.K., Khurana J.P.;
RT "Overexpression of putative topoisomerase 6 genes from rice confers stress
RT tolerance in transgenic Arabidopsis plants.";
RL FEBS J. 273:5245-5260(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
CC -!- FUNCTION: Component of the DNA topoisomerase VI involved in chromatin
CC organization and progression of endoreduplication cycles. Relaxes both
CC positive and negative superturns and exhibits a strong decatenase
CC activity. The B subunit binds ATP (By similarity). May be involved in
CC cell proliferation and stress tolerance. {ECO:0000255|HAMAP-
CC Rule:MF_03165}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_03165};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two TOP6A and two TOP6B subunits
CC (By similarity). Interacts with SPO11-2 and TOP6A3. {ECO:0000255|HAMAP-
CC Rule:MF_03165, ECO:0000269|PubMed:17116242}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03165,
CC ECO:0000269|PubMed:17116242}.
CC -!- TISSUE SPECIFICITY: Highly expressed in flowers before pollination.
CC Expressed in roots and shoots. {ECO:0000269|PubMed:17116242}.
CC -!- INDUCTION: By auxin, abscisic acid (ABA) and benzylaminopurine.
CC {ECO:0000269|PubMed:17116242}.
CC -!- SIMILARITY: Belongs to the TOP6B family. {ECO:0000255|HAMAP-
CC Rule:MF_03165}.
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DR EMBL; AJ582989; CAE47077.1; -; mRNA.
DR EMBL; CM000134; EEC84242.1; -; Genomic_DNA.
DR AlphaFoldDB; B8BDQ4; -.
DR SMR; B8BDQ4; -.
DR STRING; 39946.B8BDQ4; -.
DR EnsemblPlants; BGIOSGA030424-TA; BGIOSGA030424-PA; BGIOSGA030424.
DR Gramene; BGIOSGA030424-TA; BGIOSGA030424-PA; BGIOSGA030424.
DR HOGENOM; CLU_006403_1_0_1; -.
DR OMA; TRIELEM; -.
DR Proteomes; UP000007015; Chromosome 9.
DR GO; GO:0009330; C:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:EnsemblPlants.
DR GO; GO:0000902; P:cell morphogenesis; IEA:EnsemblPlants.
DR GO; GO:0042023; P:DNA endoreduplication; IEA:EnsemblPlants.
DR GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
DR GO; GO:0007389; P:pattern specification process; IEA:EnsemblPlants.
DR GO; GO:0009741; P:response to brassinosteroid; IEA:EnsemblPlants.
DR GO; GO:0010026; P:trichome differentiation; IEA:EnsemblPlants.
DR CDD; cd00823; TopoIIB_Trans; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR HAMAP; MF_00322; Top6B; 1.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_S5_D2-typ_fold.
DR InterPro; IPR014721; Ribosomal_S5_D2-typ_fold_subgr.
DR InterPro; IPR005734; TopoVI_B.
DR InterPro; IPR015320; TopoVI_B_transducer.
DR PANTHER; PTHR10871:SF4; PTHR10871:SF4; 1.
DR Pfam; PF09239; Topo-VIb_trans; 1.
DR SUPFAM; SSF54211; SSF54211; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA-binding; Isomerase; Nucleotide-binding; Nucleus;
KW Reference proteome; Topoisomerase.
FT CHAIN 1..696
FT /note="DNA topoisomerase 6 subunit B"
FT /id="PRO_0000421935"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 88
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03165"
FT BINDING 187
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03165"
FT BINDING 208..209
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03165"
FT BINDING 217..224
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03165"
FT BINDING 543
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03165"
FT CONFLICT 182
FT /note="R -> G (in Ref. 1; CAE47077)"
FT /evidence="ECO:0000305"
FT CONFLICT 616
FT /note="R -> H (in Ref. 1; CAE47077)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 696 AA; 78058 MW; DDA8E69D979198EF CRC64;
MDDDAGDGAA SGGTKRKVTA ASSSAAAKGK AAGKGKAASK ASALATAKES SLLKQKSPAE
FFAENKNIAG FDNPGKSLYT TMRELVENAL DSAESISELP DIEIIIEEIT KSKFNTMIGL
VDRQRIDEEL YDDFESAKAR EKRLAKEARF QETQAKNAAL GKKVKEAPAA RGKGRGEAAF
FRVTCKDNGR GMPHDDIPNM LGRVLSGTKY GLRQTRGKFG LGAKMALIWS KMSTGLPIEI
KSSMKGQNFI SFCRLDIDIH KNVPHVHLHE KRENKDRWHG AELQVIIEGN WTTHRSKILH
YMRQMAVITP YAQFLFRFLS DSPDKNLTIQ FARRTDVMPP IPLQTKHHPS AVDLLLIKRL
ISETTKQNLL QFLQHEFVNI SKSHAERLIG EMGPDFSAKT TVKSLTSQQL VRIHQLFRQA
KFDDPSGNCL SPAGEYNLRL GIIKELHPDL VATHASSPQV FEGHPFIVEA GISIGGKDVK
HGLNIFRYAN RIPLLFEQGA DVITRTALKR INWSSYKINQ QQDKIGVFVS IVSTKIPFKG
TGKEYIGDDI TEIASAVQSA LKQCCLQLKS KIVKKLQARE RQDRKRNLNR YIPDVARAIM
ETLGEIADES PPKRPRYDKE DEELLEKVNS EEVTEMTFRD CLTQHVEQVD YEMALEYAMQ
SGVSEEPREA LYLNSLEGSY KFIDFQSPVF VFRFIP
