TOPC5_PHONI
ID TOPC5_PHONI Reviewed; 80 AA.
AC P84093;
DT 16-AUG-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 44.
DE RecName: Full=U20-ctenitoxin-Pn1a;
DE Short=U20-CNTX-Pn1a;
DE AltName: Full=Omega-phonetoxin PNTx22C5;
DE Flags: Fragment;
OS Phoneutria nigriventer (Brazilian armed spider) (Ctenus nigriventer).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Lycosoidea; Ctenidae; Phoneutria.
OX NCBI_TaxID=6918;
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS
RP SPECTROMETRY.
RC TISSUE=Venom {ECO:0000269|PubMed:16278100};
RX PubMed=16278100; DOI=10.1016/j.cbpc.2005.09.010;
RA Richardson M., Pimenta A.M., Bemquerer M.P., Santoro M.M., Beirao P.S.,
RA Lima M.E., Figueiredo S.G., Bloch C. Jr., Vasconcelos E.A., Campos F.A.,
RA Gomes P.C., Cordeiro M.N.;
RT "Comparison of the partial proteomes of the venoms of Brazilian spiders of
RT the genus Phoneutria.";
RL Comp. Biochem. Physiol. 142:173-187(2006).
RN [2] {ECO:0000305}
RP FUNCTION.
RA Richardson M., Pimenta A.M.C., Bemquerer M.P., Santoro M.M.,
RA Figueiredo S.G., Cordeiro M.N.;
RT "New lethal neurotoxin PNTx22C5 from venom of the Phoneutria nigriventer
RT has sequence similarities with toxins PNTx1 and PNTx3-4 from the same
RT spider and the omega agatoxins from Agelenopsis aperta.";
RL Submitted (JUL-2004) to UniProtKB.
CC -!- FUNCTION: Omega-agatoxin are antagonists of voltage-gated calcium
CC channels (Cav). Induces rapid general flaccid paralysis followed by
CC death when injected into the cerebral ventricle of mice at dose levels
CC of 3 ug per mouse. {ECO:0000269|Ref.2}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16278100}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000269|PubMed:16278100}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- MASS SPECTROMETRY: Mass=9215.3; Mass_error=0.06; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:16278100};
CC -!- SIMILARITY: Belongs to the neurotoxin 04 (omega-agtx) family. 03 (type
CC II/III omega-agtx) subfamily. {ECO:0000305}.
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DR AlphaFoldDB; P84093; -.
DR ArachnoServer; AS000190; U20-ctenitoxin-Pn1a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR005853; Omega-agatoxin_II/III_CS.
DR InterPro; IPR013605; Toxin_34.
DR Pfam; PF08396; Toxin_34; 1.
DR PROSITE; PS60023; OMEGA_AGA_II_III; 1.
PE 1: Evidence at protein level;
KW Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Toxin;
KW Voltage-gated calcium channel impairing toxin.
FT CHAIN 1..>80
FT /note="U20-ctenitoxin-Pn1a"
FT /id="PRO_0000087612"
FT DISULFID 3..20
FT /evidence="ECO:0000305"
FT DISULFID 10..26
FT /evidence="ECO:0000305"
FT DISULFID 17..52
FT /evidence="ECO:0000305"
FT DISULFID 19..40
FT /evidence="ECO:0000305"
FT DISULFID 28..38
FT /evidence="ECO:0000305"
FT DISULFID 58..71
FT /evidence="ECO:0000255"
FT DISULFID 75..80
FT /evidence="ECO:0000255"
FT NON_TER 80
FT /evidence="ECO:0000303|Ref.2"
SQ SEQUENCE 80 AA; 8869 MW; 2FFB79AA26501C5C CRC64;
GNCIELNNDC DGSKDDCQCC RDNAYCSCYN FFGIKSGCKC SVGNSGTGYS VCLKKLECPN
RRAWTSWKKE CTKPCIGKRC
