TOPI_DROME
ID TOPI_DROME Reviewed; 814 AA.
AC Q9VH70; Q5BHW6; Q8MZ91;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Testis-specific zinc finger protein topi;
DE AltName: Full=Protein matotopetli;
GN Name=topi; ORFNames=CG8484;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAM29304.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Testis {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH COMT, AND TISSUE
RP SPECIFICITY.
RX PubMed=15084455; DOI=10.1242/dev.01032;
RA Perezgasga L., Jiang J., Bolival B. Jr., Hiller M., Benson E., Fuller M.T.,
RA White-Cooper H.;
RT "Regulation of transcription of meiotic cell cycle and terminal
RT differentiation genes by the testis-specific Zn-finger protein
RT matotopetli.";
RL Development 131:1691-1702(2004).
CC -!- FUNCTION: Required for male meiotic division and spermatid
CC differentiation. Required for accumulation of aly and comr on
CC chromatin. May function as a transcription factor.
CC {ECO:0000269|PubMed:15084455}.
CC -!- SUBUNIT: Interacts with comr. {ECO:0000269|PubMed:15084455}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15084455}. Note=Binds
CC to chromatin.
CC -!- TISSUE SPECIFICITY: Expressed in testis; primary spermatocytes.
CC {ECO:0000269|PubMed:15084455}.
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DR EMBL; AE014297; AAF54449.1; -; Genomic_DNA.
DR EMBL; AY113299; AAM29304.1; -; mRNA.
DR EMBL; BT021458; AAX33606.1; -; mRNA.
DR RefSeq; NP_649945.1; NM_141688.1.
DR AlphaFoldDB; Q9VH70; -.
DR SMR; Q9VH70; -.
DR BioGRID; 66356; 10.
DR IntAct; Q9VH70; 13.
DR STRING; 7227.FBpp0081613; -.
DR PaxDb; Q9VH70; -.
DR EnsemblMetazoa; FBtr0082135; FBpp0081613; FBgn0037751.
DR GeneID; 41199; -.
DR KEGG; dme:Dmel_CG8484; -.
DR UCSC; CG8484-RA; d. melanogaster.
DR CTD; 41199; -.
DR FlyBase; FBgn0037751; topi.
DR VEuPathDB; VectorBase:FBgn0037751; -.
DR eggNOG; KOG1721; Eukaryota.
DR HOGENOM; CLU_341096_0_0_1; -.
DR InParanoid; Q9VH70; -.
DR OMA; VNFEFAH; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9VH70; -.
DR SignaLink; Q9VH70; -.
DR BioGRID-ORCS; 41199; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41199; -.
DR PRO; PR:Q9VH70; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037751; Expressed in testis and 10 other tissues.
DR ExpressionAtlas; Q9VH70; baseline and differential.
DR Genevisible; Q9VH70; DM.
DR GO; GO:0000785; C:chromatin; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:FlyBase.
DR GO; GO:0051039; P:positive regulation of transcription involved in meiotic cell cycle; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 10.
DR SUPFAM; SSF57667; SSF57667; 4.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 10.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 8.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Meiosis;
KW Metal-binding; Nucleus; Reference proteome; Repeat; Spermatogenesis;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..814
FT /note="Testis-specific zinc finger protein topi"
FT /id="PRO_0000046929"
FT ZN_FING 228..250
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 275..297
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 360..382
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 429..453
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 467..490
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 511..533
FT /note="C2H2-type 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 539..564
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 570..592
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 598..620
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 626..649
FT /note="C2H2-type 10"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 669..705
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 161
FT /note="I -> V (in Ref. 3 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 347
FT /note="K -> E (in Ref. 3 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 383
FT /note="V -> A (in Ref. 3; AAM29304)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 814 AA; 92206 MW; 39C63AF193ED7806 CRC64;
MKVKVSGEYT LAEIEVELDQ QLTPDDLQPG ATVLATNEST GGLERIVSHE ELSRFFAVGP
AGALPMPTDV VVERTLADPA FKQILQEADG KKGFDPQAEQ MKIRDFLAGV TSSKMTTEQS
VFHGSRSNSS ASTVNRIKCP TCLVQFDAVA FQNHSCEAKP IEVAVPQQEK PHLVPTVSAP
PAPLSKPASE RVIRENQVRL RRYIKDEMKY DLATGIESSR KNAAKGPNEC TMCDRKFVHA
SGLVRHMEKH ALDLIPSQTS EQPHTIPAAG LHVVVKCNSC GRIFYDPQVA FRHGLIHDSE
HSTMRQSPMT QVPSNRADFN ELLLDGEMLI DNDPAFATSN QNTNPPKKEM FSSLILGSVL
QCEFCEYIFA DIAELLVHSA SHVAERRFEC TACDIQMNTA KEASIHFQTD CIFMREAIRS
LNVTLSRYFV CNVCELKFAN TDLLQEHRCT SFHYFPRLNE NGKKLLLPCD FCDVNFEFAH
DFLAHSEEKH LNKKKREKET RNTGAGRIRQ YLCDICGKSY TQSSHLWQHL RFHQGVKPFV
CQEENCDRKF TIRPDLNDHI RKCHTGERPY LCLVCGKRFL TGSVFYQHRL IHRGERRYEC
EECGKRFYRA DALKNHQRIH TGEKPYSCLF CTKTFRQRGD RDKHIRARHS HLDANSRLMM
QMQKFQLETA AAQKAQSHNP EQQDNDVAGG ASTSDVPSGS GFMSTEPSVA EMQYSITPEQ
QEEMVCVPID EVNNSFFMSH YMQAVPMEED GSGQHIIVFE QPGQNMDMMS IYDQQQVGEP
MHESGVPKRP AEENARVVVV KNNPTKPIFS DTYL
