ID   TOPZ1_SHEEP             Reviewed;        1599 AA.
AC   E5FYH0; W5P2E5;
DT   21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT   25-APR-2018, sequence version 2.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Protein TOPAZ1 {ECO:0000305};
DE   AltName: Full=Testis- and ovary-specific PAZ domain-containing protein 1 {ECO:0000303|PubMed:22069478};
GN   Name=TOPAZ1;
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Caprinae; Ovis.
OX   NCBI_TaxID=9940;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP   DEVELOPMENTAL STAGE.
RC   TISSUE=Embryonic ovary;
RX   PubMed=22069478; DOI=10.1371/journal.pone.0026950;
RA   Baillet A., Le Bouffant R., Volff J.N., Luangpraseuth A., Poumerol E.,
RA   Thepot D., Pailhoux E., Livera G., Cotinot C., Mandon-Pepin B.;
RT   "TOPAZ1, a novel germ cell-specific expressed gene conserved during
RT   evolution across vertebrates.";
RL   PLoS ONE 6:E26950-E26950(2011).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Texel;
RX   PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA   Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA   Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA   Wang W., Xun X.;
RT   "The sheep genome reference sequence: a work in progress.";
RL   Anim. Genet. 41:449-453(2010).
CC   -!- FUNCTION: Important for normal spermatogenesis and male fertility.
CC       Specifically required for progression to the post-meiotic stages of
CC       spermatocyte development. Seems to be necessary for normal expression
CC       levels of a number of testis-expressed gene transcripts, although its
CC       role in this process is unclear. {ECO:0000250|UniProtKB:E5FYH1}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:22069478}.
CC   -!- TISSUE SPECIFICITY: Expressed in both adult testis and fetal ovary,
CC       mostly in germ cells (at protein level). {ECO:0000269|PubMed:22069478}.
CC   -!- DEVELOPMENTAL STAGE: Expression increases in the ovary during the
CC       meiosis prophase I period at 55-65 dpc and decreases until the end of
CC       the fetal life and then remains very low in adult ovary. Low expression
CC       in testis throughout fetal development. {ECO:0000269|PubMed:22069478}.
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DR   EMBL; HM631979; ADK36690.1; -; mRNA.
DR   EMBL; AMGL01044446; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AMGL01044447; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AMGL01044448; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; E5FYH0; -.
DR   STRING; 9940.ENSOARP00000004594; -.
DR   PRIDE; E5FYH0; -.
DR   eggNOG; ENOG502QPIV; Eukaryota.
DR   HOGENOM; CLU_003190_0_0_1; -.
DR   Proteomes; UP000002356; Unplaced.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
DR   InterPro; IPR038952; TOPAZ1.
DR   InterPro; IPR029435; TOPAZ1_dom.
DR   InterPro; IPR000571; Znf_CCCH.
DR   PANTHER; PTHR35671; PTHR35671; 1.
DR   Pfam; PF14669; Asp_Glu_race_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Differentiation; Reference proteome; Spermatogenesis.
FT   CHAIN           1..1599
FT                   /note="Protein TOPAZ1"
FT                   /id="PRO_0000416095"
FT   REGION          1..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          159..185
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          802..836
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          867..889
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        10..26
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        807..830
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        867..884
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        324
FT                   /note="Y -> C (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        333
FT                   /note="V -> L (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        797
FT                   /note="L -> M (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        971
FT                   /note="L -> V (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1289
FT                   /note="G -> R (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1299..1302
FT                   /note="KIHC -> LKIHF (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1349
FT                   /note="N -> I (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1463..1464
FT                   /note="HY -> PH (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1476
FT                   /note="L -> S (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1539
FT                   /note="Y -> H (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1590..1591
FT                   /note="GV -> AG (in Ref. 1; ADK36690)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1599 AA;  180026 MW;  9B08E9EC095E7CE0 CRC64;
     MVAQASGKEV ESDKSAKEKR KVTEASSDDP QPGIDLVRKE SLTSSESFPT VECSEFQSMA
     FLQSLGKERL VEGIKRRIRI KKFKSLESPA LKMTENKATQ NSKVEFQDEL YKNTLKYSCN
     SLSPGVENNS TLKLHDCSCL SHSKDCNDEN NLAYKPDGGC MHVPENSSKS KKENPRSLID
     KTDPSNIPQL LQTEENLMRV SQLLLEENDS YLSKNNGMFS CLQSEKNKHS IEESSIGRKS
     RKRMKVSEKG NGMVIEMKFS NMCNKSELML QGNQTGAEGK ETETLEAKKS SLKVLRKVNN
     NTLSPMDPLL SLPETGKKTS PEHYANAVFQ KAVEQLSKEE TKNVSQPLGC TSMDPPEDYF
     KSMKNSLVKS LSDCFPIEKR SSREGLKNEA EESKYSCHRT IPMTGKRTWP CYSCARISAQ
     CWKKASLPQS SRQDDFLRHQ MNQTHLSDSK LMLQSSVTET NSASSSIEKL DSNLNCLPSV
     STVEPTSVVI KEPIVNDDEK MKSEELSRSA SEVVSNITED TPLTNVTHNS TGSKKKDRGN
     LTKLNLMVAS QDGQEASNST GKTVHRKACI TKQALVVPDL VKILNTGRLT NFKIPLLKNK
     TEKRKEINAK SSEREVYSPL ELLDSLSGAE VRQSRTKENT VTVTSGPQSL SIQHSVIPVQ
     ASSDSFCSKN SCIIAPSFLK QGNNNKPSSH ISASGHIISN KAAGSLTVEN NTFSCDPGCI
     EKNPTFYSNE QEPFKAVSSE VSGRKMSKNF SEIKVGFPDI LKAYEDDVLL IDVIQDDPDL
     FGVSSEGELS FPSEVPLISQ EPNVAEEHQS ADSKHMELPE KKEPSDHLRE LPVPDPGSVK
     SEICASLSAA SEIKHDSKDA NISLGEVTHE TSSNEKPGGL SEQTKSSDLD EKCRFSDKVA
     IREEKETISE VFRSDSKNTE IMVGECHLAA LVSKPLCLPV PLPPLNLSTH QEDTLLNPWM
     NDFRLPGKHS LLKLQNPEIC EIFKREKNLG VFQKPLGLII PHRYCKFHFN TIRGCERSQC
     KFAHVPEQGD EKVCMDVFKK YISISELCLL QRAANMFMEY YRKFLPGIHF DLQVLNDLLN
     SLLKHCLLKE VFQIMNLCIM IKMLPALKIL LKIFEYVATM KLRNAVPALI DMFCKLVEAG
     MVLDLEHFNY IVKLLYQVQA SKQEITAVLE MKSRLRMRQF KKNWKCDLEA ALNEIEHCKE
     KGDWTKLGNV YLNIKMSCEK FADFQRFCAY IAEILTKDCK EERPGVPFCE FAETVSKDLQ
     NSEVDKTLLG RIGISAMYFY HKLLQWSKGR KVLDKLYEKI HCTSLKGLIG PEKLAPRCQI
     VNIAAEIFLK SGSLDGAIWV LRESEWIINT PVWPCDRLDV LNRHNLLCTI AHEILAKSLY
     RQTFEVLQNL PGFQNSQETV EVSQYSLLFN KLLDACIESN SLGMSSSVAE FMISKSIPID
     FSFLRRLITS LGRSCLWLKA RAHYKSALSL GCYPPLEGNL YRKLLLIPSY LSEIEMLLAI
     EIFLVSNASS IQSPGTSTQM LQIVLKRSEE NKSRSKDDYQ AAVERLIMAA RISDPKLFIK
     HMTVNVNKEQ VYSLEHCSAL KWLKENMKWG VKVWLFNNR