TOS2_YEAST
ID TOS2_YEAST Reviewed; 622 AA.
AC P50078; D6VV03;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Protein TOS2;
DE AltName: Full=Target of SBF 2;
GN Name=TOS2; OrderedLocusNames=YGR221C; ORFNames=G8523;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8701610;
RX DOI=10.1002/(sici)1097-0061(19960330)12:4<385::aid-yea910>3.0.co;2-g;
RA van der Aart Q.J.M., Kleine K., Steensma H.Y.;
RT "Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1
RT region from the right arm of Saccharomyces cerevisiae chromosome VII.";
RL Yeast 12:385-390(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 69 AND 91.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION BY CDC28.
RX PubMed=14574415; DOI=10.1038/nature02062;
RA Ubersax J.A., Woodbury E.L., Quang P.N., Paraz M., Blethrow J.D., Shah K.,
RA Shokat K.M., Morgan D.O.;
RT "Targets of the cyclin-dependent kinase Cdk1.";
RL Nature 425:859-864(2003).
RN [6]
RP FUNCTION IN ANCHORING OF CDC24.
RX PubMed=14872283; DOI=10.1007/s00294-004-0485-9;
RA Toenjes K.A., Simpson D., Johnson D.I.;
RT "Separate membrane targeting and anchoring domains function in the
RT localization of the S. cerevisiae Cdc24p guanine nucleotide exchange
RT factor.";
RL Curr. Genet. 45:257-264(2004).
CC -!- FUNCTION: Seems to be involved in the anchoring of CDC24 to the
CC membrane of polarized growth sites. {ECO:0000269|PubMed:14872283}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}. Bud membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- PTM: Phosphorylated by CDC28. {ECO:0000269|PubMed:14574415}.
CC -!- MISCELLANEOUS: Present with 56 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the SKG6/TOS2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA61169.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=CAA97249.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X87941; CAA61169.1; ALT_FRAME; Genomic_DNA.
DR EMBL; Z73006; CAA97249.1; ALT_FRAME; Genomic_DNA.
DR EMBL; BK006941; DAA08314.2; -; Genomic_DNA.
DR PIR; S57684; S57684.
DR RefSeq; NP_011737.2; NM_001181350.2.
DR AlphaFoldDB; P50078; -.
DR BioGRID; 33474; 59.
DR DIP; DIP-2699N; -.
DR IntAct; P50078; 2.
DR MINT; P50078; -.
DR STRING; 4932.YGR221C; -.
DR iPTMnet; P50078; -.
DR PaxDb; P50078; -.
DR PRIDE; P50078; -.
DR EnsemblFungi; YGR221C_mRNA; YGR221C; YGR221C.
DR GeneID; 853136; -.
DR KEGG; sce:YGR221C; -.
DR SGD; S000003453; TOS2.
DR VEuPathDB; FungiDB:YGR221C; -.
DR GeneTree; ENSGT00940000176525; -.
DR HOGENOM; CLU_026020_0_0_1; -.
DR InParanoid; P50078; -.
DR OMA; EYEDATD; -.
DR BioCyc; YEAST:G3O-30902-MON; -.
DR PRO; PR:P50078; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P50078; protein.
DR GO; GO:0005933; C:cellular bud; HDA:SGD.
DR GO; GO:0033101; C:cellular bud membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005935; C:cellular bud neck; IDA:SGD.
DR GO; GO:0005934; C:cellular bud tip; IDA:SGD.
DR GO; GO:0000131; C:incipient cellular bud site; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0032507; P:maintenance of protein location in cell; IGI:SGD.
DR GO; GO:1902413; P:negative regulation of mitotic cytokinesis; IMP:SGD.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..622
FT /note="Protein TOS2"
FT /id="PRO_0000072633"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 181..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..205
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 622 AA; 69471 MW; 63F4D79A4B09A6DC CRC64;
MFSHYRYKEN SCQKREAIPD KSRVSLTFLQ KRTDSSNVTV AVAVAVPIGA IIIVLSVVLI
VVYRRCKKEP SMQDFDPNFE GDLYYLPKMD SSMNSANSDS NATEKRFIYG GYDDFLQPSI
ENSQSFKDYV RRINEHAPSA YNIASLASQN NSKLSVPSKH IDLSNKISFE SLENSELIVS
PQHSNTGQDC DQRCDSTSNP DVNEKSSHNN DNRLKSNYTS RSGLEPQCSR EEEENIDRIR
SIYNIYFEKS NSTIRSSVTS SIRRDSKLNI ATRKSVNMSS QDNPNDTTLI EQSHFGSTTV
QEIDSSSSAN EEYEDATDYL QVPAPQENKN IASSVYSEVA TREKVIPESS LSLTFPPPNG
LSTRITSSIY SDTVAKDHIH SAKAPVRALS EGSGQSNLTS AQQYSTYFID HCNQSNDDNY
YYNYPLPLEH PQNYENIGDL PTPTQFIYST SSHSLTSFKG RPKPPKTLKH VPTARLNGTA
LNPMDHPEMF YSSPTKIPST SLTKQFCTPL PYQLRQSVVM TNPSELSMKP RYKPAGSLRN
LIKAQYLPGN SSTTTSSSLS QPPSTLSNAI NFRVSGLLDD TDILQPPSVG EILPFKASTE
DLRKQLGTSH NYEITPYENV HV
