ID   TOS6_YEAS1              Reviewed;         102 AA.
AC   B3LPD6;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   02-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Protein TOS6;
DE   Flags: Precursor;
GN   Name=TOS6; ORFNames=SCRG_03425;
OS   Saccharomyces cerevisiae (strain RM11-1a) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=285006;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RM11-1a;
RG   The Broad Institute Genome Sequencing Platform;
RA   Birren B.W., Lander E.S., Galagan J.E., Nusbaum C., Devon K., Cuomo C.,
RA   Jaffe D.B., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M.,
RA   Mauceli E.W., Brockman W., MacCallum I.A., Rounsley S., Young S.K.,
RA   LaButti K., Pushparaj V., DeCaprio D., Crawford M., Koehrsen M., Engels R.,
RA   Montgomery P., Pearson M., Howarth C., Larson L., Luoma S., White J.,
RA   O'Leary S., Kodira C.D., Zeng Q., Yandava C., Alvarado L., Pratt S.,
RA   Kruglyak L.;
RT   "Annotation of the Saccharomyces cerevisiae RM11-1a genome.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. Membrane
CC       {ECO:0000305}; Lipid-anchor, GPI-anchor {ECO:0000305}.
CC   -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC       reticulum and serves to target the protein to the cell surface. There,
CC       the glucosamine-inositol phospholipid moiety is cleaved off and the
CC       GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC       glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TOS6 family. {ECO:0000305}.
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DR   EMBL; CH408049; EDV12531.1; -; Genomic_DNA.
DR   AlphaFoldDB; B3LPD6; -.
DR   EnsemblFungi; EDV12531; EDV12531; SCRG_03425.
DR   HOGENOM; CLU_2279081_0_0_1; -.
DR   Proteomes; UP000008335; Unassembled WGS sequence.
DR   GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Secreted;
KW   Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..80
FT                   /note="Protein TOS6"
FT                   /id="PRO_0000402236"
FT   PROPEP          81..102
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000402237"
FT   LIPID           80
FT                   /note="GPI-anchor amidated glycine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        69
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   102 AA;  10170 MW;  2F8D02993704E1F5 CRC64;
     MKFSTLSTVA AIAAFASADS TSDGVTYVDV TTTPQSTTSM VSTVKTTSTP YTTSTIATLS
     TKSISSQANT TTHEISTYVG AAVKGSVAGM GAIMGAAAFA LL