TOS6_YEAST
ID TOS6_YEAST Reviewed; 102 AA.
AC P48560; D6W0P4;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Protein TOS6;
DE Flags: Precursor;
GN Name=TOS6; OrderedLocusNames=YNL300W; ORFNames=N0430;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8553702; DOI=10.1002/yea.320111311;
RA Maurer K.C.T., Urbanus J.H.M., Planta R.J.;
RT "Sequence analysis of a 30 kb DNA segment from yeast chromosome XIV
RT carrying a ribosomal protein gene cluster, the genes encoding a plasma
RT membrane protein and a subunit of replication factor C, and a novel
RT putative serine/threonine protein kinase gene.";
RL Yeast 11:1303-1310(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=9613572; DOI=10.1007/s004380050706;
RA Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.;
RT "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall
RT proteins in Saccharomyces cerevisiae.";
RL Mol. Gen. Genet. 258:53-59(1998).
RN [6]
RP INDUCTION.
RX PubMed=10770760; DOI=10.1128/aac.44.5.1255-1265.2000;
RA Bammert G.F., Fostel J.M.;
RT "Genome-wide expression patterns in Saccharomyces cerevisiae: comparison of
RT drug treatments and genetic alterations affecting biosynthesis of
RT ergosterol.";
RL Antimicrob. Agents Chemother. 44:1255-1265(2000).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP INDUCTION.
RX PubMed=16254148; DOI=10.1126/science.1120499;
RA Tu B.P., Kudlicki A., Rowicka M., McKnight S.L.;
RT "Logic of the yeast metabolic cycle: temporal compartmentalization of
RT cellular processes.";
RL Science 310:1152-1158(2005).
RN [9]
RP DISRUPTION PHENOTYPE.
RX PubMed=16911514; DOI=10.1111/j.1567-1364.2006.00089.x;
RA Kawahata M., Masaki K., Fujii T., Iefuji H.;
RT "Yeast genes involved in response to lactic acid and acetic acid: acidic
RT conditions caused by the organic acids in Saccharomyces cerevisiae cultures
RT induce expression of intracellular metal metabolism genes regulated by
RT Aft1p.";
RL FEMS Yeast Res. 6:924-936(2006).
RN [10]
RP SUBCELLULAR LOCATION.
RX PubMed=19054390; DOI=10.1111/j.1600-0854.2008.00857.x;
RA Castillon G.A., Watanabe R., Taylor M., Schwabe T.M., Riezman H.;
RT "Concentration of GPI-anchored proteins upon ER exit in yeast.";
RL Traffic 10:186-200(2009).
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305|PubMed:19054390,
CC ECO:0000305|PubMed:9613572}. Membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
CC -!- INDUCTION: Expression decreases in response to ergosterol perturbation
CC or upon entry into stationary phase. {ECO:0000269|PubMed:10770760,
CC ECO:0000269|PubMed:16254148}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer (By
CC similarity). {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Increases resistance to lactic acid.
CC {ECO:0000269|PubMed:16911514}.
CC -!- MISCELLANEOUS: Present with 1480 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the TOS6 family. {ECO:0000305}.
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DR EMBL; U23084; AAC49098.1; -; Genomic_DNA.
DR EMBL; Z71576; CAA96218.1; -; Genomic_DNA.
DR EMBL; AY692649; AAT92668.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10260.1; -; Genomic_DNA.
DR PIR; S60400; S60400.
DR RefSeq; NP_014099.1; NM_001183138.1.
DR AlphaFoldDB; P48560; -.
DR BioGRID; 35538; 73.
DR DIP; DIP-4464N; -.
DR STRING; 4932.YNL300W; -.
DR PaxDb; P48560; -.
DR EnsemblFungi; YNL300W_mRNA; YNL300W; YNL300W.
DR GeneID; 855416; -.
DR KEGG; sce:YNL300W; -.
DR SGD; S000005244; TOS6.
DR VEuPathDB; FungiDB:YNL300W; -.
DR eggNOG; ENOG502SCUF; Eukaryota.
DR HOGENOM; CLU_2279081_0_0_1; -.
DR InParanoid; P48560; -.
DR OMA; CSGGCTP; -.
DR BioCyc; YEAST:G3O-33288-MON; -.
DR PRO; PR:P48560; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P48560; protein.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:SGD.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..80
FT /note="Protein TOS6"
FT /id="PRO_0000203371"
FT PROPEP 81..102
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000372450"
FT LIPID 80
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 69
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 102 AA; 10170 MW; 2F8D02993704E1F5 CRC64;
MKFSTLSTVA AIAAFASADS TSDGVTYVDV TTTPQSTTSM VSTVKTTSTP YTTSTIATLS
TKSISSQANT TTHEISTYVG AAVKGSVAGM GAIMGAAAFA LL
