TOST1_CAEEL
ID TOST1_CAEEL Reviewed; 142 AA.
AC Q18490;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Protein tost-1 {ECO:0000305};
DE AltName: Full=Twenty one u antagonist 1 {ECO:0000303|PubMed:31216475};
GN Name=tost-1 {ECO:0000303|PubMed:31147388, ECO:0000312|WormBase:C35D10.13};
GN ORFNames=C35D10.13 {ECO:0000312|WormBase:C35D10.13};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE PETISCO COMPLEX, INTERACTION WITH ERH-2;
RP PID-3 AND TOFU-6, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=31216475; DOI=10.1016/j.celrep.2019.05.076;
RA Zeng C., Weng C., Wang X., Yan Y.H., Li W.J., Xu D., Hong M., Liao S.,
RA Dong M.Q., Feng X., Xu C., Guang S.;
RT "Functional Proteomics Identifies a PICS Complex Required for piRNA
RT Maturation and Chromosome Segregation.";
RL Cell Rep. 27:3561-3572(2019).
RN [3] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE PETISCO COMPLEX, INTERACTION WITH ERH-2,
RP DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ARG-42.
RX PubMed=31147388; DOI=10.1101/gad.322446.118;
RA Cordeiro Rodrigues R.J., de Jesus Domingues A.M., Hellmann S., Dietz S.,
RA de Albuquerque B.F.M., Renz C., Ulrich H.D., Sarkies P., Butter F.,
RA Ketting R.F.;
RT "PETISCO is a novel protein complex required for 21U RNA biogenesis and
RT embryonic viability.";
RL Genes Dev. 33:857-870(2019).
CC -!- FUNCTION: Component of the tost-1 variant of the PETISCO complex which
CC plays an essential role in embryogenesis (PubMed:31147388,
CC PubMed:31216475). Within the complex acts as an adapter which binds to
CC the complex via erh-2 (PubMed:31147388). Does not seem to play a role
CC in the biogenesis of a class of 21 nucleotide PIWI-interacting RNAs
CC (piRNAs) that possess a uracil residue at the 5'-end (also called 21U-
CC RNAs) (PubMed:31147388). May inhibit 21U-RNA accumulation
CC (PubMed:31216475). Required for chromosome segregation and cell
CC division in early embryos (PubMed:31216475).
CC {ECO:0000269|PubMed:31147388, ECO:0000269|PubMed:31216475,
CC ECO:0000305|PubMed:31216475}.
CC -!- SUBUNIT: Component of the tost-1 variant of the PETISCO complex (also
CC called the pid-3, erh-2, tofu-6, and ife-3 small RNA complex)
CC containing at least tost-1, tofu-6, ife-3, pid-3, and erh-2, which
CC plays an essential role in embryogenesis (PubMed:31147388,
CC PubMed:31216475). Within the complex interacts with erh-2
CC (PubMed:31216475, PubMed:31147388). Within the complex interacts with
CC pid-3 and tofu-6 (PubMed:31216475). In contrast to the pid-1 variant of
CC the PETISCO complex, the tost-1 variant of the PETISCO complex plays a
CC minor role in the biogenesis of a class of 21 nucleotide PIWI-
CC interacting RNAs (piRNAs) that possess a uracil residue at the 5'-end
CC (also called 21U-RNAs) (PubMed:31147388). {ECO:0000269|PubMed:31147388,
CC ECO:0000269|PubMed:31216475, ECO:0000305|PubMed:31216475}.
CC -!- INTERACTION:
CC Q18490; Q20057: erh-2; NbExp=3; IntAct=EBI-21447173, EBI-21447087;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:31216475}. Nucleus
CC {ECO:0000269|PubMed:31216475}. Note=During early embryogenesis,
CC localizes to the nucleus at prophase of cell division, and remains in
CC the cytosol at interphase in 2- and 4-cell embryos (PubMed:31216475).
CC Mainly localizes to the germline cytoplasm (PubMed:31216475).
CC {ECO:0000269|PubMed:31216475}.
CC -!- TISSUE SPECIFICITY: Expressed in the germline.
CC {ECO:0000269|PubMed:31216475}.
CC -!- DEVELOPMENTAL STAGE: During early embryogenesis, expressed during
CC prophase and interphase in 2- and 4-cell embryos.
CC {ECO:0000269|PubMed:31216475}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryonic
CC lethality (PubMed:31147388). RNAi-mediated knockdown results in
CC chromosome segregation and cell division defects in early embryos
CC (PubMed:31216475). RNAi-mediated knockdown enhances the activity of the
CC PIWI-interacting RNA (piRNA) silencing pathway (PubMed:31147388). RNAi-
CC mediated knockdown disrupts the localization of tofu-6 to the
CC perinuclear region of the germline (PubMed:31216475). RNAi-mediated
CC knockdown results in larger tofu-6-, erh-2- and pid-3-expressing foci
CC (PubMed:31216475). {ECO:0000269|PubMed:31147388,
CC ECO:0000269|PubMed:31216475}.
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DR EMBL; BX284603; CCD66786.1; -; Genomic_DNA.
DR PIR; S72577; S72577.
DR RefSeq; NP_498019.1; NM_065618.1.
DR PDB; 7EJO; X-ray; 2.19 A; A=34-54.
DR PDBsum; 7EJO; -.
DR AlphaFoldDB; Q18490; -.
DR SMR; Q18490; -.
DR ComplexPortal; CPX-4307; PETISCO, tost-1 variant.
DR IntAct; Q18490; 4.
DR STRING; 6239.C35D10.13; -.
DR EPD; Q18490; -.
DR PaxDb; Q18490; -.
DR PeptideAtlas; Q18490; -.
DR EnsemblMetazoa; C35D10.13.1; C35D10.13.1; WBGene00016449.
DR GeneID; 183241; -.
DR KEGG; cel:CELE_C35D10.13; -.
DR UCSC; C35D10.13; c. elegans.
DR CTD; 183241; -.
DR WormBase; C35D10.13; CE01194; WBGene00016449; tost-1.
DR eggNOG; ENOG502TI4E; Eukaryota.
DR HOGENOM; CLU_1866923_0_0_1; -.
DR InParanoid; Q18490; -.
DR OMA; DKENAHV; -.
DR OrthoDB; 1591290at2759; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00016449; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
DR GO; GO:0034518; C:RNA cap binding complex; IPI:ComplexPortal.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IC:ComplexPortal.
DR GO; GO:0051781; P:positive regulation of cell division; IMP:UniProtKB.
DR GO; GO:0051984; P:positive regulation of chromosome segregation; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Chromosome partition; Cytoplasm;
KW Nucleus; Reference proteome.
FT CHAIN 1..142
FT /note="Protein tost-1"
FT /id="PRO_0000452469"
FT REGION 97..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 97..122
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 42
FT /note="May be required for interaction with erh-2"
FT /evidence="ECO:0000305|PubMed:31147388"
FT MUTAGEN 42
FT /note="R->C: May abolish the interaction with erh-2."
FT /evidence="ECO:0000269|PubMed:31147388"
FT HELIX 39..47
FT /evidence="ECO:0007829|PDB:7EJO"
SQ SEQUENCE 142 AA; 16221 MW; 3394085F84A5C294 CRC64;
MTTAIQYQVI PLADREHSNK VASILENKYK TATNKRITLN ERFGVLEKGY TIQATEPETV
KNDSDVFFVV HDRNLNSKKE FVENYLAKNP IVNSPEKVFE EEDKENAPTK KAVLKPSSTD
EKKLTSSDIY IQEITRSILQ SV
