TOTA_DROME
ID TOTA_DROME Reviewed; 129 AA.
AC Q8IN44; Q95NS3;
DT 25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein Turandot A;
DE Flags: Precursor;
GN Name=TotA {ECO:0000312|EMBL:AAN13840.1, ECO:0000312|FlyBase:FBgn0028396};
GN ORFNames=CG31509;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAK64523.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RC STRAIN=Canton-S {ECO:0000312|EMBL:AAK64523.1};
RC TISSUE=Pupae {ECO:0000269|PubMed:11409894};
RX PubMed=11409894; DOI=10.1006/bbrc.2001.5067;
RA Ekengren S., Hultmark D.;
RT "A family of Turandot-related genes in the humoral stress response of
RT Drosophila.";
RL Biochem. Biophys. Res. Commun. 284:998-1003(2001).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAK40253.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
RC STRAIN=Canton-S {ECO:0000312|EMBL:AAK40253.1};
RX PubMed=11369236; DOI=10.1016/s0960-9822(01)00203-2;
RA Ekengren S., Tryselius Y., Dushay M.S., Liu G., Steiner H., Hultmark D.;
RT "A humoral stress response in Drosophila.";
RL Curr. Biol. 11:714-718(2001).
RN [3] {ECO:0000312|EMBL:AAN13840.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAN13840.1}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5] {ECO:0000312|EMBL:AAR31120.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAR31120.1}; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=12967563; DOI=10.1016/s1534-5807(03)00244-2;
RA Agaisse H., Petersen U.-M., Boutros M., Mathey-Prevot B., Perrimon N.;
RT "Signaling role of hemocytes in Drosophila JAK/STAT-dependent response to
RT septic injury.";
RL Dev. Cell 5:441-450(2003).
RN [7] {ECO:0000305}
RP FUNCTION, AND INDUCTION.
RX PubMed=16611243; DOI=10.1111/j.1365-2443.2006.00953.x;
RA Brun S., Vidal S., Spellman P., Takahashi K., Tricoire H., Lemaitre B.;
RT "The MAPKKK Mekk1 regulates the expression of Turandot stress genes in
RT response to septic injury in Drosophila.";
RL Genes Cells 11:397-407(2006).
CC -!- FUNCTION: A humoral factor that plays a role in stress tolerance; gives
CC increased resistance to the lethal effects of bacterial challenge and
CC stress. Regulated by the JAK/STAT pathway and NF-KB-like Relish pathway
CC in the fat body, upd3 in the hemocytes and Mekk1 in response to septic
CC injury and consequent immune response. {ECO:0000269|PubMed:11369236,
CC ECO:0000269|PubMed:11409894, ECO:0000269|PubMed:12967563,
CC ECO:0000269|PubMed:16611243}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11369236,
CC ECO:0000269|PubMed:11409894, ECO:0000269|PubMed:12967563}.
CC Note=Secreted rom the fat body into the hemolymph.
CC -!- TISSUE SPECIFICITY: Expressed in the fat body (at protein level).
CC {ECO:0000269|PubMed:11369236, ECO:0000269|PubMed:12967563}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the third larval instar and
CC maintained through pupal development. Levels gradually increase during
CC adulthood. {ECO:0000269|PubMed:11369236, ECO:0000269|PubMed:11409894}.
CC -!- INDUCTION: By stressful conditions such as bacterial infection, heat
CC shock, mechanical pressure, dehydration, feeding with oxidative agents
CC such as paraquat or exposure to ultraviolet light.
CC {ECO:0000269|PubMed:11369236, ECO:0000269|PubMed:11409894,
CC ECO:0000269|PubMed:12967563, ECO:0000269|PubMed:16611243}.
CC -!- MISCELLANEOUS: Flies overexpressing TotA show prolonged survival and
CC retain normal activity at otherwise lethal temperatures.
CC {ECO:0000269|PubMed:11409894}.
CC -!- SIMILARITY: Belongs to the Turandot family. {ECO:0000305}.
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DR EMBL; AY035990; AAK64523.1; -; mRNA.
DR EMBL; AY029600; AAK40253.1; -; mRNA.
DR EMBL; AY029601; AAK40254.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13840.1; -; Genomic_DNA.
DR EMBL; BT011049; AAR31120.1; -; mRNA.
DR RefSeq; NP_536778.2; NM_080517.3.
DR AlphaFoldDB; Q8IN44; -.
DR SMR; Q8IN44; -.
DR STRING; 7227.FBpp0083378; -.
DR GlyGen; Q8IN44; 1 site.
DR PaxDb; Q8IN44; -.
DR PRIDE; Q8IN44; -.
DR DNASU; 44121; -.
DR EnsemblMetazoa; FBtr0083971; FBpp0083378; FBgn0028396.
DR GeneID; 44121; -.
DR KEGG; dme:Dmel_CG31509; -.
DR UCSC; CG31509-RA; d. melanogaster.
DR CTD; 44121; -.
DR FlyBase; FBgn0028396; TotA.
DR VEuPathDB; VectorBase:FBgn0028396; -.
DR GeneTree; ENSGT00940000176310; -.
DR HOGENOM; CLU_152780_0_0_1; -.
DR InParanoid; Q8IN44; -.
DR OMA; CCAYSDA; -.
DR OrthoDB; 1607084at2759; -.
DR PhylomeDB; Q8IN44; -.
DR BioGRID-ORCS; 44121; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 44121; -.
DR PRO; PR:Q8IN44; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0028396; Expressed in head capsule and 19 other tissues.
DR ExpressionAtlas; Q8IN44; baseline and differential.
DR Genevisible; Q8IN44; DM.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0034605; P:cellular response to heat; IEP:FlyBase.
DR GO; GO:0071260; P:cellular response to mechanical stimulus; IEP:FlyBase.
DR GO; GO:0034599; P:cellular response to oxidative stress; IEP:FlyBase.
DR GO; GO:0034644; P:cellular response to UV; IEP:FlyBase.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0009617; P:response to bacterium; IDA:UniProtKB.
DR GO; GO:0009409; P:response to cold; IDA:UniProtKB.
DR GO; GO:0009408; P:response to heat; IDA:UniProtKB.
DR GO; GO:0009612; P:response to mechanical stimulus; IDA:UniProtKB.
DR GO; GO:0046689; P:response to mercury ion; IMP:FlyBase.
DR GO; GO:0051597; P:response to methylmercury; IMP:FlyBase.
DR GO; GO:0006979; P:response to oxidative stress; IDA:UniProtKB.
DR GO; GO:0009411; P:response to UV; IDA:UniProtKB.
DR GO; GO:0009414; P:response to water deprivation; IDA:UniProtKB.
DR InterPro; IPR010825; Turandot.
DR Pfam; PF07240; Turandot; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Glycoprotein; Immunity; Innate immunity;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..129
FT /note="Protein Turandot A"
FT /id="PRO_0000355137"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 31
FT /note="N -> S (in Ref. 1; AAK64523 and 2; AAK40253/
FT AAK40254)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 129 AA; 14220 MW; FB879758944E5754 CRC64;
MNSSTALMCF ALLLISPLCM GYSDEDREAD NLRIAEIIKN AQDDDSKINS TQELLDIYRR
LYPSLTPEER ESIDKFVNEH TDAIIIDGVP IQGGRKARIV GKIVSPGVKG LATGFFEELG
SKLAQLFTG
