TOX1_OXYTA
ID TOX1_OXYTA Reviewed; 123 AA.
AC P83288; W0LP48;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 2.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Omega-oxotoxin-Ot1a;
DE Short=Omega-OXTX-Ot1a;
DE AltName: Full=Oxytoxin-1 {ECO:0000303|PubMed:24462682};
DE Short=OxyTx1 {ECO:0000303|PubMed:24462682};
DE AltName: Full=Tx-1 {ECO:0000312|EMBL:AHG24516.1};
DE Flags: Precursor;
OS Oxyopes takobius (Lynx spider) (Oxyopes foliiformis).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Lycosoidea; Oxyopidae; Oxyopes.
OX NCBI_TaxID=666126;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Venom gland;
RX PubMed=24462682; DOI=10.1016/j.febslet.2014.01.018;
RA Sachkova M.Y., Slavokhotova A.A., Grishin E.V., Vassilevski A.A.;
RT "Genes and evolution of two-domain toxins from lynx spider venom.";
RL FEBS Lett. 588:740-745(2014).
RN [2]
RP PROTEIN SEQUENCE OF 55-123, FUNCTION, SUBCELLULAR LOCATION, TOXIC DOSE,
RP MASS SPECTROMETRY, AND CIRCULAR DICHROISM ANALYSIS.
RC TISSUE=Venom;
RX PubMed=11976325; DOI=10.1074/jbc.m200511200;
RA Corzo G., Villegas E., Gomez-Lagunas F., Possani L.D., Belokoneva O.S.,
RA Nakajima T.;
RT "Oxyopinins, large amphipathic peptides isolated from the venom of the wolf
RT spider Oxyopes kitabensis with cytolytic properties and positive
RT insecticidal cooperativity with spider neurotoxins.";
RL J. Biol. Chem. 277:23627-23637(2002).
CC -!- FUNCTION: Weak blocker of vertebrate P/Q-, N- and L-type voltage-gated
CC calcium channels (Cav1 and Cav2) (By similarity). Is both paralytic and
CC lethal when injected into lepidopteran larvae. Is not toxic to mice.
CC {ECO:0000250, ECO:0000269|PubMed:11976325}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11976325}.
CC -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC {ECO:0000305|PubMed:11976325}.
CC -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC structurally defines this protein as a knottin. {ECO:0000305}.
CC -!- PTM: Mass spectrometry data suggest a carboxylated free C-terminal
CC residue.
CC -!- MASS SPECTROMETRY: Mass=8059.25; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:11976325};
CC -!- TOXIC DOSE: LD(50) is 5.1 +- 0.5 nmol/g on lepidopteran larvae
CC (Spodoptera litura). {ECO:0000269|PubMed:11976325}.
CC -!- MISCELLANEOUS: The primary structure of the mature peptide is identical
CC to that of omega-oxotoxin-Ol1a from Oxyopes lineatus (AC P0C8M0).
CC -!- SIMILARITY: Belongs to the spiderine family. Spiderine subfamily.
CC {ECO:0000305}.
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DR EMBL; KF766562; AHG24516.1; -; mRNA.
DR AlphaFoldDB; P83288; -.
DR ArachnoServer; AS000779; omega-oxotoxin-Ot1a.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005246; F:calcium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR044061; OXYTX_ICK.
DR PROSITE; PS51861; OXYTX_ICK; 1.
PE 1: Evidence at protein level;
KW Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Knottin; Neurotoxin; Secreted; Signal; Toxin;
KW Voltage-gated calcium channel impairing toxin.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT PROPEP 17..54
FT /evidence="ECO:0000269|PubMed:11976325"
FT /id="PRO_0000444424"
FT CHAIN 55..123
FT /note="Omega-oxotoxin-Ot1a"
FT /evidence="ECO:0000269|PubMed:11976325"
FT /id="PRO_0000087676"
FT DOMAIN 55..122
FT /note="Oxytoxin-type inhibitor cystine knot (ICK)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01208"
FT DISULFID 58..72
FT /evidence="ECO:0000250|UniProtKB:P86716"
FT DISULFID 65..77
FT /evidence="ECO:0000250|UniProtKB:P86716"
FT DISULFID 69..118
FT /evidence="ECO:0000250|UniProtKB:P86716"
FT DISULFID 71..106
FT /evidence="ECO:0000250|UniProtKB:P86716"
FT DISULFID 79..104
FT /evidence="ECO:0000250|UniProtKB:P86716"
SQ SEQUENCE 123 AA; 14065 MW; E6154B3568B180B4 CRC64;
MKIVLVFVCT LYLAQATYLS EQDVNEVSEF LEALDQANEA ASEMVEAAET EEARDWECLP
LHSSCDNDCV CCKNHHCHCP YSNVSKLEKW LPEWAKIPDA LKRCSCQRND KDGKINTCDK
YKN