TOX3_MOUSE
ID TOX3_MOUSE Reviewed; 575 AA.
AC Q80W03; Q8BIV8;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=TOX high mobility group box family member 3;
DE AltName: Full=Trinucleotide repeat-containing gene 9 protein;
GN Name=Tox3; Synonyms=Tnrc9;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcriptional coactivator of the p300/CBP-mediated
CC transcription complex. Activates transactivation through cAMP response
CC element (CRE) sites. Protects against cell death by inducing
CC antiapoptotic and repressing pro-apoptotic transcripts. Stimulates
CC transcription from the estrogen-responsive or BCL-2 promoters. Required
CC for depolarization-induced transcription activation of the C-FOS
CC promoter in neurons. Associates with chromatin to the estrogen-
CC responsive C3 promoter region (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Interacts (via HGM box) with CITED1 (via C-
CC terminus); the interaction increases estrogen-response element (ERE)-
CC dependent transcription and protection against cell death. Interacts
CC with CREB1 (phosphorylated form). Interacts with CREB1; the interaction
CC is not depolarization dependent. Interacts with CREBBP (via C-terminus)
CC (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q80W03; Q8VHS6: Asb15; NbExp=2; IntAct=EBI-26675915, EBI-26675998;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DOMAIN: the C-terminus is required for calcium responsiveness but not
CC for transactivation activity. {ECO:0000250}.
CC -!- DOMAIN: The N-terminus is absolutely necessary for transactivation
CC activity. {ECO:0000250}.
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DR EMBL; AK082602; BAC38545.1; -; mRNA.
DR EMBL; BC052044; AAH52044.1; -; mRNA.
DR CCDS; CCDS22516.1; -.
DR RefSeq; NP_766501.2; NM_172913.3.
DR AlphaFoldDB; Q80W03; -.
DR SMR; Q80W03; -.
DR BioGRID; 232663; 9.
DR IntAct; Q80W03; 8.
DR STRING; 10090.ENSMUSP00000105250; -.
DR iPTMnet; Q80W03; -.
DR PhosphoSitePlus; Q80W03; -.
DR MaxQB; Q80W03; -.
DR PaxDb; Q80W03; -.
DR PRIDE; Q80W03; -.
DR ProteomicsDB; 258954; -.
DR Antibodypedia; 28333; 345 antibodies from 24 providers.
DR DNASU; 244579; -.
DR Ensembl; ENSMUST00000109621; ENSMUSP00000105250; ENSMUSG00000043668.
DR GeneID; 244579; -.
DR KEGG; mmu:244579; -.
DR UCSC; uc009msb.2; mouse.
DR CTD; 27324; -.
DR MGI; MGI:3039593; Tox3.
DR VEuPathDB; HostDB:ENSMUSG00000043668; -.
DR eggNOG; KOG0381; Eukaryota.
DR GeneTree; ENSGT00940000158043; -.
DR HOGENOM; CLU_030650_1_0_1; -.
DR InParanoid; Q80W03; -.
DR OMA; QQHHMQL; -.
DR OrthoDB; 1465513at2759; -.
DR PhylomeDB; Q80W03; -.
DR TreeFam; TF106481; -.
DR BioGRID-ORCS; 244579; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Tox3; mouse.
DR PRO; PR:Q80W03; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q80W03; protein.
DR Bgee; ENSMUSG00000043668; Expressed in rostral migratory stream and 200 other tissues.
DR ExpressionAtlas; Q80W03; baseline and differential.
DR Genevisible; Q80W03; MM.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0031490; F:chromatin DNA binding; IBA:GO_Central.
DR GO; GO:0051219; F:phosphoprotein binding; ISO:MGI.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0003713; F:transcription coactivator activity; ISO:MGI.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0019722; P:calcium-mediated signaling; ISO:MGI.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISS:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0042981; P:regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.30.10; -; 1.
DR InterPro; IPR009071; HMG_box_dom.
DR InterPro; IPR036910; HMG_box_dom_sf.
DR Pfam; PF00505; HMG_box; 1.
DR SMART; SM00398; HMG; 1.
DR SUPFAM; SSF47095; SSF47095; 1.
DR PROSITE; PS50118; HMG_BOX_2; 1.
PE 1: Evidence at protein level;
KW Activator; Apoptosis; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..575
FT /note="TOX high mobility group box family member 3"
FT /id="PRO_0000286354"
FT DNA_BIND 254..322
FT /note="HMG box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00267"
FT REGION 189..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 516..575
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..217
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..239
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 10
FT /note="A -> G (in Ref. 1; BAC38545)"
FT /evidence="ECO:0000305"
FT CONFLICT 14
FT /note="A -> S (in Ref. 1; BAC38545)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 575 AA; 63174 MW; E1B95AD9F3BCFC91 CRC64;
MDVRFYPAAA GDPAGLDFAQ CLGYYGYSKL GNNNYMNMAE ANNAFFAASE QTFHTPSLGD
EEFEIPPITP PPESDPTLGM PDALLPFQTL SDPLPSQGTE FTPQFPPQSL DLPSITISRN
LVEQDGVLHS NGLHMDQSHT QVSQYRQDPS LVMRSIVHMT DGARSGIMPP AQLTTINQSQ
LSAQLGLNLG GANVSHTSPS PPASKSATPS PSSSINEEDA DDANRAIGEK RTAPDSGKKP
KTPKKKKKKD PNEPQKPVSA YALFFRDTQA AIKGQNPNAT FGEVSKIVAS MWDSLGEEQK
QVYKRKTEAA KKEYLKALAA YRASLVSKAA AESAEAQTIR SVQQTLASTN LTSSLLLNTS
LSQHGTVPAS PQTLPQSLPR SIAPKPLTMR LPMSQIVTSV TIAANMPSNI GAPLISSMGT
TMVGSATSTQ VSPSVQTQQH QMQLQQQQQQ QQQMQQMQQQ QLQQHQMHQQ IQQQMQQQHF
QHHMQQHLQQ QQQQHLQQQL SQQQLQQQLQ QHLQLQQLQH MQHQSQPSPR QHSPVTSQIT
SPIPAIGSPQ PASQQHQPQI QSQTQTQVLP QVSIF
